Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Ge, Bo; Guo, Chao; Liang, Yuanying; Liu, Meizhen; Wu, Ka

doi:10.1002/biof.1558

Cited by 21 publications

(13 citation statements)

References 26 publications

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“…The R packages of ‘ClusterProfiler’, ‘ReactomePA’ and ‘AnnotationHub’ were used for enrichment analysis of Gene Ontology (GO) in biological function (BP) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway using the previously identified best targets. This data were used to generate a corresponding bubble chart and histogram [ 26 ]. GO and KEGG analyses were performed using clusterProfiler (version 3.14.3) [ 27 ] and ReactomePA (version 1.30.0) [ 28 ] from the R packages.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic targets and signaling mechanisms of vitamin C activity against sepsis: a bioinformatics study

Li¹,

Guo²,

Yu³

et al. 2020

Briefings in Bioinformatics

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Sepsis is a life-threatening complication of pneumonia, including coronavirus disease-2019 (COVID-19)-induced pneumonia. Evidence of the benefits of vitamin C (VC) for the treatment of sepsis is accumulating. However, data revealing the targets and molecular mechanisms of VC action against sepsis are limited. In this report, a bioinformatics analysis of network pharmacology was conducted to demonstrate screening targets, biological functions, and the signaling pathways of VC action against sepsis. As shown in network assays, 63 primary causal targets for the VC action against sepsis were identified from the data, and four optimal core targets for the VC action against sepsis were identified. These core targets were epidermal growth factor receptor (EGFR), mitogen-activated protein kinase-1 (MAPK1), proto-oncogene c (JUN), and signal transducer and activator of transcription-3 (STAT3). In addition, all biological processes (including a top 20) and signaling pathways (including a top 20) potentially involved in the VC action against sepsis were identified. The hub genes potentially involved in the VC action against sepsis and interlaced networks from the Kyoto Encyclopedia of Genes and Genomes Mapper assays were highlighted. Considering all the bioinformatic findings, we conclude that VC antisepsis effects are mechanistically and pharmacologically implicated with suppression of immune dysfunction-related and inflammation-associated functional processes and other signaling pathways. These primary predictive biotargets may potentially be used to treat sepsis in future clinical practice.

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Section: Methodsmentioning

confidence: 99%

Therapeutic targets and signaling mechanisms of vitamin C activity against sepsis: a bioinformatics study

Li¹,

Guo²,

Yu³

et al. 2020

Briefings in Bioinformatics

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“…The Animal Guidelines for the Care and Use of Animals at Guilin Medical University were approved. After acclimatization for approximate 7‐day, we prepared the CG mice chemically using our previous method 13,14 . And then CG mice were treated intragastrically with 20, 40 mg/kg PA each day for lasting 14‐day.…”

Section: Methodsmentioning

confidence: 99%

“…Encouragingly, our previous reports show that some potential anti‐CG agents, including glycyrrhetinic acid, vitamin C, are found with potential pharmacological actions against CG. The underlying mechanisms of glycyrrhetinic acid against CG are associated with inhibition of cell death and inflammatory stress, while the anti‐CG mechanism of vitamin C is related to reduction of intracellular tumor necrosis factor (TNF)‐α, IL6, and c‐Jun expressions 13,14 . Currently, the association between PA and CG is uninvestigated.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics and experimental findings reveal the therapeutic actions and targets of pachymic acid against cystitis glandularis

Shi

Liang

et al. 2021

BioFactors

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Pachymic acid (PA), a bioactive ingredient isolated from Poria cocos Wolf, is reported with potential benefits of anti‐inflammatory, anti‐oxidative actions. It is reasoned that PA may play the potential benefits against cystitis glandularis (CG), an inflammation of the bladder tissue. In this study, we aimed to apply the network pharmacology and molecular docking analyses to reveal concrete anti‐CG targets and mechanisms of PA, and then the bioinformatic findings were verified by using clinical and animal samples. The methodological data from network pharmacology approach showed that 303 and 243 reporting targets of CG and PA, and other 31 shared targets of CG and PA were identified. Subsequently, all top targets of PA against CG were screened out, including cyclooxygenase‐2, epidermal growth factor receptor, tumor antigen p53 (TP53), tumor necrosis factor‐alpha (TNF), interleukin‐1 (IL‐1) beta, proto‐oncogene c‐jun. Molecular docking data demonstrated that PA exerted potent bonding capacities with TNF, TP53 proteins in CG. In human study, the findings suggested that overactivated TNF‐α expression and suppressed TP53 activation were detected in CG samples. In animal study, PA‐treated mice showed reduced intravesical IL‐1, IL‐6 levels, and lactate dehydrogenase content, downregulated TNF‐α and upregulated TP53 proteins in bladder samples. Taken together, our bioinformatics and experimental findings identify the key anti‐CG biotargets and mechanisms of PA. More markedly, these pivotal pharmacological targets of PA against CG have been screened out and verified by using computational and experimental analyses.

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“…PPI network diagram was drawn by Cytoscape_v3.6.0. The core targets were selected according to the node degree value ( 27 , 28 ).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle

Cheng

Yang

et al. 2021

Front. Vet. Sci.

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The fruits of Ailanthus altissima Swingle (AS) possess a variety of pharmacological activities. Its antioxidant activity and the potential mode of action have not yet been investigated. In in vitro studies, AS revealed the strong reducing power and DPPH scavenging effect, but hydroxyl radical scavenging activity and ferrous ions-chelating ability were not strong. Meanwhile, the oxidative stress RAW264.7 cell injury model was established, the low and medium-doses of AS showed significant protective effects on the viability of H2O2-treated cells by CCK-8 method. Besides, three doses of AS all increased the activities of SOD, CAT, and GSH-Px and decreased the MDA level compared with the H2O2 group, suggesting it significantly relieved oxidative stress of cells. The active ingredients and related targets of AS were collected by HERB and Swiss Target Prediction database, the common targets of drugs and diseases database were conducted by GeneCards database platform and the Venny platform. We screened the core targets of AS like threonine kinase1 (AKT1), mitogen-activated protein kinase 1 (MAPK1), sirtuin-1 (SIRT1), mechanistic target of rapamycin kinase (MTOR) by STRING database, and the key pathways involved PI3K-AKT and FoxO signaling pathway by KEGG pathway enrichment analysis. Besides, qRT-PCR revealed AS preconditioning significantly up-regulated the expression level of AKT1, SIRT1, MAPK1, and MTOR in model cells, and the effect was related to the regulation of FoxO and PI3K/AKT signaling pathway. In summary, AS showed significant antioxidant activity and its potential mechanism was regulating FoxO and PI3K/AKT signaling pathway.

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Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Cited by 21 publications

References 26 publications

Therapeutic targets and signaling mechanisms of vitamin C activity against sepsis: a bioinformatics study

Therapeutic targets and signaling mechanisms of vitamin C activity against sepsis: a bioinformatics study

Bioinformatics and experimental findings reveal the therapeutic actions and targets of pachymic acid against cystitis glandularis

Antioxidant Activity and the Potential Mechanism of the Fruit From Ailanthus altissima Swingle

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