“…It has been demonstrated in Vitro that these compounds inhibit many DNA-dependent enzymatic functions associated with DNA replication or RNA transcription. Enzymes affected include DNA and RNA polymerases and topoisomerases (Puschendorf & Grunicke, 1969;Zimmer et al, 1971b;Wahnert et al, 1975;Woynarowski et al, 1989;Mortensen et al, 1990;McHugh et al, 1990;Ueno et al, 1992), as well as DNA gyrase and helicase (Bachur et al, 1993;Storl et al, 1993). Recent studies, moreover, have illustrated the ability of these groove-binding drugs to prevent the binding of transcription factors (Gambari et al, 1991;Dorn et al, 1992;Chiang et al, 1994;Welch et al, 1994).…”