Nestin-Lineage Cells Contribute to the Microvasculature but Not Endocrine Cells of the Islet

Treutelaar, Mary K.; Skidmore, Jennifer; Dias-Leme, Claudia L.; Hara, Manami; Zhang, Lizhi; Simeone, Diane M.; Martin, Donna M.; Burant, Charles F.

doi:10.2337/diabetes.52.10.2503

Cited by 132 publications

(101 citation statements)

References 45 publications

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“…4F,G) and of PC2 Ϫ/Ϫ , PC2 ϩ/ϩ , and Gcgr Ϫ/Ϫ mice (not shown) contained nestin ϩ cells that did not express hormones. These cells are likely to be the mesenchymal nestin ϩ cells described by others (Delacour et al, 2004;Esni et al, 2004;Humphrey et al, 2003;Klein et al, 2003;Lardon et al, 2002;Treutelaar et al, 2003).…”

Section: Reappearance Of Nestin Expression In Pancreatic Cells Of Adumentioning

confidence: 69%

“…This possibility was supported by the discovery of nestin ϩ cells in epithelial cells of the pancreatic bud of mouse embryos from e-10 to e-15 (Esni et al, 2004), raising the prospect that these cells were endocrine precursor cells. This possibility is controversial since several reports indicated that nestin expression was restricted to cells derived from the mesenchyme (Humphrey et al, 2003;Klein et al, 2003;Lardon et al, 2002;Selander and Edlund, 2002;Treutelaar et al, 2003). The derivation of pancreatic endocrine and exocrine cells from nestin precursors is further complicated by conflicting results obtained from cell lineage analysis of pancreatic cells using molecular marking techniques.…”

Section: Introductionmentioning

confidence: 99%

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Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Kedees¹,

Guz²,

Vuguin³

et al. 2007

Developmental Dynamics

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Nestin, a marker of neural stem cells, is also expressed by cells located in the epithelium of the pancreatic primordium and by a subpopulation of exocrine cells but not by endocrine cells. These findings raised the possibility that the pancreatic epithelium is heterogeneous and comprised of subpopulations of exocrine/ nestin-positive and endocrine/nestin-negative precursor cells. We examined this issue in two mutant mouse models characterized by protracted expression of several embryonal properties in islet cells. One mutant line comprises mice lacking mature glucagon due to abrogation of proprotein convertase-2 (PC2 ؊/؊ ), responsible for the conversion of proglucagon into glucagon, while the second line consists of mice with a global deletion of the glucagon receptor (Gcgr ؊/؊ ). We demonstrate that nestin is transiently expressed by acinar cells and by insulin and glucagon cells of islets of both lines of mice. In addition, the lack of glucagon signaling increased nestin mRNA levels in pancreas of mutant embryos and adult mice. We conclude that nestin؉ cells located in the pancreatic primordium generate the cells of the endocrine and exocrine lineages. Furthermore, our results suggest that nestin expression is regulated by glucagon signaling.

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Section: Reappearance Of Nestin Expression In Pancreatic Cells Of Adumentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Kedees¹,

Guz²,

Vuguin³

et al. 2007

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…These studies require further confirmation, as other investigators found that nestin was a marker for non-endocrine cells. 47,48 Seaberg et al 49 reported clonal identification of multipotent pancreatic precursors from adult mouse pancreas that differentiate in vitro into both islet endocrine and neural lineages. Interestingly, these islet lineage clones display glucoseresponsive insulin secretion.…”

Section: Ex Vivo Conversion Of Cells Into Functional B-cellsmentioning

confidence: 99%

Gene Therapy Progress and Prospects: Gene therapy for diabetes mellitus

Yechoor

Chan

2004

Gene Ther

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Diabetes mellitus has long been targeted, as yet unsuccessfully, as being curable with gene therapy. The main hurdles have not only been vector-related toxicity but also the lack of physiological regulation of the expressed insulin. Recent advances in understanding the developmental biology of b-cells and the transcriptional cascade that drives it have enabled both in vivo and ex vivo gene therapy combined with cell therapy to be used in animal models of diabetes with success. The associated developments in the stem cell biology and immunology have opened up further opportunities for gene therapy to be applied to target autoimmune diabetes.

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“…While nestin protein is robustly expressed in the developing CNSd in which it appears during the time of CNS stem and progenitor cell proliferation and neuronal migrationdit subsequently decreases as the brain develops (Lendahl et al, 1990;Tohyama et al, 1992;Dahlstrand et al, 1995;Kojima et al, 2004). Postnatal expression of nestin is limited to the brain subventricular (SVZ) zone and endothelial cells (Treutelaar et al, 2003;Mokry et al, 2004;Romanko et al, 2004;Ernst and Christie, 2005;Tonchev et al, 2005). Re-expression of nestin has recently been observed in activated astrocytes following various pathological conditions both in vitro and in vivo (Schmidt-Kastner and Humpel, 2002;Wei et al, 2002;Lang et al, 2004;Namba et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…However, even in normal development, there is emerging evidence that nestin is not exclusivley restricted to neural progenitor/stem cells, as its presence has been reported in multiple lineage-committed cell types, including cardiac muscle, lens-epithelial cells, multipotent pancreatic stem cells, Schwann cells, microglia cells and oligodendrocytes (Zimmerman et al, 1994;Yang et al, 2000;Vanderwinden et al, 2002;Treutelaar et al, 2003;Yokoyama et al, 2004;Amoh et al, 2005). In addition, nestin co-expression with early or late stage neuronal phenotypic markers, such as beta-tubulin-III and MAP-2, has also been reported at a certain stage of neuronal development (Rosser et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Revisiting nestin expression in retinal progenitor cells in vitro and after transplantation in vivo

Qiu

Seiler

Thomas

et al. 2007

Experimental Eye Research

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The purpose of this study is to characterize the co-expression of nestinda neuroectodermal stem cell and a reactive glial markerdwith various mature retinal cell markers in retinal progenitor cells (RPCs) expanded in vitro, followed either by in vitro induction or subretinal transplantation. Rat RPCs derived from embryonic day (E) 17 rat retina were expanded in serum free defined culture, and induced to differentiate by alltrans retinoic acid (RA). Following induction, cells were stained for nestin in combination with retinal neuronal and glial markers. Cultured cells were collected for quantitative RT-PCR gene expression analysis prior to and after induction. In a second series, passage 2 RPCs were transplanted into the subretinal space of S334ter-3 retinal degeneration rats at postnatal day 28. After 1e4 weeks, sections through the transplant were double immunostained for nestin and various retinal specific neuronal markers. The cultured RPCs treated with RA exhibited nestin co-expression with various retinal specific markers, including protein kinase C a (PKC), neurofilament 200 (NF200), cellular retinaldehyde binding protein (CRALBP), and rhodopsin. Following RA induction, quantitative RT-PCR analysis demonstrated downregulation of nestin, PAX-6, thy1.1, and PKCa, and upregulation of rhodopsin, glial fibrillary acidic protein (GFAP), and CrX. No nestin coexpression was observed with any of the retinal specific neuronal markers in RPC transplants in vivo except for some nestin-immunoreactivity overlapping with GFAP positive cells in the host retina. The role of nestin as a unique neural stem/progenitor cell marker should be reconsidered. Nestin expression during RPC maturation appears to be different in vitro versus in vivo.

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Nestin-Lineage Cells Contribute to the Microvasculature but Not Endocrine Cells of the Islet

Cited by 132 publications

References 45 publications

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Gene Therapy Progress and Prospects: Gene therapy for diabetes mellitus

Revisiting nestin expression in retinal progenitor cells in vitro and after transplantation in vivo

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