Nested Polymerase Chain Reaction Validated for Sensitive Detection of Bovine Leukemia Virus in Blood Samples From Brazilian Cattle Herds: Comparison With Conventional Elisa and Agar Gel Immunodiffusion Methods
L. Gregory,
P.S. Carneiro,
E.H. Birgel Junior
et al.
Abstract:A sensitive and specific nested polymerase chain reaction (nested PCR) for detection of bovine leukemia virus (BLV) in blood samples was evaluated as a reliable assay for accurate diagnosis of naturally infected animals under field conditions. The standardized nested PCR relies on the identification of BLV DNA fragments of 340 or 444 bp produced by applying 2 different pairs of primers sets to the proviral env gene. This protocol was validated by testing 40 bovine blood samples from a BLV-positive dairy herd, … Show more
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