Nested interactions in the combined toxicity of uranium and cadmium to the nematode Caenorhabditis elegans

Margerit, A.; Lecomte-Pradines, Catherine; Svendsen, Claus; Frelon, Sandrine; Góméz, Elena; Gilbin, Rodolphe

doi:10.1016/j.ecoenv.2015.04.023

Cited by 18 publications

(5 citation statements)

References 42 publications

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“…This result is different from a simple synergistic/antagonistic effect, where there is a dose level-dependent deviation (DL) because the two reference models deviate differently at low versus high dose levels [ 64 ]. This conclusion is similar to that of Margerit et al [ 56 ], whose study on the combined toxicity of U and Cd for the nematode Caenorhabditis elegans found that low dose levels were synergistic and high dose levels were antagonistic. When Cd was mixed with Cu, the predicted results were overall similar to those for the U-Cd mixture, namely, synergistic effects and better IA model predictions, with both metals producing toxicity in a mutually independent form.…”

Section: Discussionsupporting

confidence: 91%

“…One of the main bioavailable forms of U is the uranyl cation UO 2 2+ [ 55 ]. Competition between UO 2 2+ and Cd 2+ for one transporter depends on their concentration, affinity for the transporter and internalization kinetics, and differences in the concentration levels of U and Cd can lead to a reduction in Cd competition [ 56 ]. In this study, the concentration of U was at least 18 times higher than Cd, with an average design concentration of approximately 190 times, and this high concentration difference led to a reduction in the toxicity produced by Cd in the presence of U.…”

Section: Discussionmentioning

confidence: 99%

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Sensitivity of Ostracods to U, Cd and Cu: The Case of Cypridopsis vidua

Chen

Huo

et al. 2022

Toxics

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The development of uranium mines has been necessary to obtain abundant and scarce uranium resources, but they also bring inevitable radioactive contamination to the surrounding soil, rivers and lakes. This paper explores the sensitivity of Cypridopsis vidua to the radioactive element uranium and the heavy elements cadmium and copper with single and combined acute toxicity experiments and combined toxicity model predictions. The results from the single toxicity experiments showed that the degree of toxic effects was cadmium > copper > uranium. The combined toxicity experiments showed that the compound toxicity of U-Cd and U-Cu was higher than the weakest component and lower than the strongest component, whereas the compound toxicity of Cd-Cu was higher than either of its components. When the overall proportion of a more toxic metal was increased, its mixed toxicity also increased, and vice versa. Combined toxicity predictions showed that the U-Cd combination was best described by the concentration additive (CA) model, the independent action (IA) model was more applicable to the Cd-Cu combination, and the most applicable model for the U-Cu combination changed depending on the concentration gradient. The acute toxicity data from this study provide a reference for the development of wastewater discharge standards for uranium mines, enriches the data related to the toxicity of uranium for ostracods and deepens the understanding of the threat of uranium pollution to aquatic ecosystems.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Sensitivity of Ostracods to U, Cd and Cu: The Case of Cypridopsis vidua

Chen

Huo

et al. 2022

Toxics

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“…The nematodes were cultured at 15 °C, 70 % relative humidity in darkness, on NGM agar seeded with Escherichia coli OP50 strain, according to the standard method [55]. Bacteria were prepared following the standard protocol [56]. Seeded plates were exposed to UVC (20 min, Bio-LinkCrosslinker; 254 nm, μWm2) to suppress bacterial activity [57].…”

Section: Strain and Maintenancementioning

confidence: 99%

Interplay between ionizing radiation effects and aging in C. elegans

Kuzmić

Galas

Lecomte-Pradines

et al. 2019

Free Radical Biology and Medicine

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Living species are chronically exposed to environmental ionizing radiations from sources that can be overexpressed by nuclear accidents. In invertebrates, reproduction is the most radiosensitive studied endpoint, likely to be connected with aging. Surprisingly, aging is a sparsely investigated endpoint after chronic ionizing radiation, whereas understanding it is of fundamental interest in biology and medicine. Indeed, aging and aging-related diseases (e.g., cancer and degenerative diseases) cause about 90 % of deaths in developed countries. Therefore, glp-1 sterile Caenorhabditis elegans nematode was used to assess the impact of chronic gamma irradiation on the lifespan. Analyses were performed, at the individual level, on aging and, in order to delve deeper into the mechanisms, at the molecular level, on oxidative damage (carbonylation), biomolecules (lipids, proteins and nucleic acids) and their colocalization. We observed that ionizing radiation accelerates aging (whatever the duration (3 to 19 days)/dose (0.5 to 24 Gy)/dose rate (7 and 52 mGy.h-1) tested) leading to a longevity value equivalent to that of wt nematode (~25-30 days). Moreover, the level of protein oxidative damage (carbonylation) turned out to be good cellular biomarker of aging, since it increases with age. Conversely, chronic radiation treatments reduced carbonylation levels and induced neutral lipid catabolism whatever the dose rate and the final delivered dose. Finally, under some conditions a lipidprotein colocalization without any carbonyl was observed; this could be linked to yolk accumulation in glp-1 nematodes. To conclude, we noticed through this study a link between chronic gamma exposure, lifespan shortening and lipid level decrease associated with a decrease in the overall carbonylation.

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“…Organisms and populations can respond to stress using a wide range of mechanisms from a behavioural response like a change of resting site by ectotherms according to the temperature stress, an acclimation response involving changes in enzymatic activities allowing a complete or partial recovery (re-establishment of the homeostasis), or even a genetic adaptation by natural selection of genetic variants presenting a higher metabolic efficiency9. Over the past 15 years, several studies have attempted to assess the effects of mixtures of chemicals1011121314, mixtures of ecological factors1516 or, mixtures of chemicals and ecological factors171819 on organisms and populations. These studies indicate that the prediction of the environmental effects of multiple stressors is a challenge that is beginning to be addressed by the scientific community.…”

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confidence: 99%

Integrated presentation of ecological risk from multiple stressors

Goussen

Price

Rendal

et al. 2016

Sci Rep

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Current environmental risk assessments (ERA) do not account explicitly for ecological factors (e.g. species composition, temperature or food availability) and multiple stressors. Assessing mixtures of chemical and ecological stressors is needed as well as accounting for variability in environmental conditions and uncertainty of data and models. Here we propose a novel probabilistic ERA framework to overcome these limitations, which focusses on visualising assessment outcomes by construct-ing and interpreting prevalence plots as a quantitative prediction of risk. Key components include environmental scenarios that integrate exposure and ecology, and ecological modelling of relevant endpoints to assess the effect of a combination of stressors. Our illustrative results demonstrate the importance of regional differences in environmental conditions and the confounding interactions of stressors. Using this framework and prevalence plots provides a risk-based approach that combines risk assessment and risk management in a meaningful way and presents a truly mechanistic alternative to the threshold approach. Even whilst research continues to improve the underlying models and data, regulators and decision makers can already use the framework and prevalence plots. The integration of multiple stressors, environmental conditions and variability makes ERA more relevant and realistic.

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Nested interactions in the combined toxicity of uranium and cadmium to the nematode Caenorhabditis elegans

Cited by 18 publications

References 42 publications

Sensitivity of Ostracods to U, Cd and Cu: The Case of Cypridopsis vidua

Sensitivity of Ostracods to U, Cd and Cu: The Case of Cypridopsis vidua

Interplay between ionizing radiation effects and aging in C. elegans

Integrated presentation of ecological risk from multiple stressors

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