Nested collagen matrices: A new model to study migration of human fibroblast populations in three dimensions

Grinnell, Frederick; Rocha, Lenaldo Branco; Iucu, Cristina; Rhee, Sangmyung; Jiang, Hongmei

doi:10.1016/j.yexcr.2005.10.001

Cited by 75 publications

(117 citation statements)

References 66 publications

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“…In contrast, 3-D matrices provided spatial advantages for cell-cell and cell-matrix adhesion as well as support for cell traction 38 . This is a behavior similar than fibroblast in 2D and 3D collagen substrates, classically reported 39,40 .…”

Section: Cell Morphology By Light Microscopysupporting

confidence: 67%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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Chitosan is soluble in diluted acid solutions and can easily form films by casting. However, residual acid neutralization should be performed for biomedical applications what may compromise physical and mechanical properties of the films. Thus, plasticizers can be added to improve these properties. The aim of this study was to characterize morphological, barrier and mechanical properties, besides evaluate the in vitro cytotoxicity of sorbitol-plasticized and NaOH-Na 2 CO 3 neutralized chitosan membranes for skin substitute application. Scanning electron microscopy, X-ray diffraction, water vapor permeability and mechanical tests were carried out to characterize the obtained membranes. Moreover, Vero cells were used for in vitro cytotoxicity evaluation. In this paper, we report a non-cytotoxic sorbitol-plasticized chitosan membrane with desirable properties for skin substitution, such as flexibility, water vapor permeability and high percentage of elongation.

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Section: Cell Morphology By Light Microscopysupporting

confidence: 67%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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“…Recent advances in multiple particle-tracking rheology (23,24) as well as the use of nested collagen matrices (25,26) to probe tension in the proximity of cells have shown great promise in improving our understanding of cell-matrix interactions at these length scales.…”

Section: Resultsmentioning

confidence: 99%

Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis

Zaman

Trapani

Sieminski

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,053

1,005

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Cell migration on 2D surfaces is governed by a balance between counteracting tractile and adhesion forces. Although biochemical factors such as adhesion receptor and ligand concentration and binding, signaling through cell adhesion complexes, and cytoskeletal structure assembly/disassembly have been studied in detail in a 2D context, the critical biochemical and biophysical parameters that affect cell migration in 3D matrices have not been quantitatively investigated. We demonstrate that, in addition to adhesion and tractile forces, matrix stiffness is a key factor that influences cell movement in 3D. Cell migration assays in which Matrigel density, fibronectin concentration, and β1 integrin binding are systematically varied show that at a specific Matrigel density the migration speed of DU-145 human prostate carcinoma cells is a balance between tractile and adhesion forces. However, when biochemical parameters such as matrix ligand and cell integrin receptor levels are held constant, maximal cell movement shifts to matrices exhibiting lesser stiffness. This behavior contradicts current 2D models but is predicted by a recent force-based computational model of cell movement in a 3D matrix. As expected, this 3D motility through an extracellular environment of pore size much smaller than cellular dimensions does depend on proteolytic activity as broad-spectrum matrix metalloproteinase (MMP) inhibitors limit the migration of DU-145 cells and also HT-1080 fibrosarcoma cells. Our experimental findings here represent, to our knowledge, discovery of a previously undescribed set of balances of cell and matrix properties that govern the ability of tumor cells to migration in 3D environments.

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“…After a lag phase of several hr, fibroblasts with leading dendritic extensions (inset, H&E stained section), can be observed. See Figures 1-3 in [72] for additional details. Model showing convergence of PDGF and LPA signaling on PAK1 and cofilin1, cell ruffling, and collagen matrix contraction.…”

Section: Discussionmentioning

confidence: 99%

“…Fibroblasts were shown to migrate out of dermal equivalents into fibrin matrices [71], so we developed a similar model to study growth factor specificity of fibroblast migration in collagen matrices [72]. Figure 5 illustrates the nested collagen matrix model and shows the border between the inner dermal equivalent and surrounding outer matrix.…”

Section: Multiple Mechanisms Of Collagen Matrix Remodelingmentioning

confidence: 99%

Fibroblast mechanics in 3D collagen matrices☆

Rhee

Grinnell²

2007

Advanced Drug Delivery Reviews

Self Cite

166

115

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Connective tissues provide mechanical support and frameworks for the other tissues of the body. Type 1 collagen is the major protein component of ordinary connective tissue, and fibroblasts are the cell type primarily responsible for its biosynthesis and remodeling. Research on fibroblasts interacting with collagen matrices explores all four quadrants of cell mechanics: pro-migratory vs. pro-contractile growth factor environments on one axis; high tension vs. low tension cell-matrix interactions on the other. The dendritic fibroblast -probably equivalent to the resting tissue fibroblast -can be observed only in the low tension quadrant and generally has not been appreciated from research on cells incubated with planar culture surfaces. Fibroblasts in the low tension quadrant require microtubules for formation of dendritic extensions, whereas fibroblasts in the high tension quadrant require microtubules for polarization but not for spreading. Ruffling of dendritic extensions rather than their overall protrusion or retraction provides the mechanism for remodeling of floating collagen matrices, and floating matrix remodeling likely reflects a model of tissue mechanical homeostasis.

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Nested collagen matrices: A new model to study migration of human fibroblast populations in three dimensions

Cited by 75 publications

References 66 publications

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis

Fibroblast mechanics in 3D collagen matrices☆

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