Nested clade analysis and the genetic evidence for population expansion in the phylogeography of the golden-striped salamander, Chioglossa lusitanica (Amphibia: Urodela)

Abstract

The golden-striped salamander, Chioglossa lusitanica, is a streamside species distributed in the northwest of the Iberian Peninsula. We gathered cytochrome b mitochondrial DNA sequence data from samples across the species range and used nested clade distance analysis to assess evolutionary processes explaining the geographical distribution of mtDNA diversity in Chioglossa lusitanica. A significant association was observed between genetic structure and geography. The geographical patterns were explained by past fragmentation and restricted gene flow. Inferences were concordant

Phylogeographic evidence for the existence of an ancient biogeographic barrier: the Isthmus of Kra Seaway

“…Templeton's (2004) latest inference key was used to infer processes involved in any statistically significant associations observed. It has recently been suggested that some NCA inferences may be flawed, and should therefore be supported by the use of alternative analytical techniques (eg Alexandrino et al, 2002;Masta et al, 2003;Templeton, 2004). We therefore applied the coalescent/maximum-likelihood approach implemented in FLUCTUATE ver.…”

A molecular assessment of phylogenetic relationships and lineage accumulation rates within the family Salamandridae (Amphibia, Caudata)

“…This included a data set of 12S-tRNA Val -16S sequence for 32 ingroup taxa and 5 outgroups (Caccone et al, 1994;Steinfartz et al, 2002;Titus and Larson, 1995;Zajc and Arntzen, 1999) and a data set of Cytochrome b sequences for 32 ingroup taxa and 2 outgroups (Alexandrino et al, 2002;Caccone et al, 1994;Chan et al, 2001;Chippindale et al, 2001;García-París et al, 2003;Hedges et al, 1992;Tan and Wake, 1995). Sequences in the 12S-tRNA Val -16S region range from approximately 300-1000 bp in length.…”

High mitochondrial diversity within and among populations of Malagasy poison frogs

“…Analysing a fragment of only 329 bp in 17 populations of the goldenstriped salamander Chioglossa lusitanica, Alexandrino et al (2000) found 1-4 haplotypes per population (mean 1.76), with a sequence divergence between haplotypes of up to 3.2% and an overall nucleotide diversity of 1.4%. A subsequent study in which 700 bp was sequenced in 124 individuals from 17 localities identified 30 haplotypes with sequence divergence ranging from 0.14 to 2.14% and an overall nucleotide diversity of 0.99% (Alexandrino et al, 2002). They found that 10 out of 12 localities with samples of n > 5 were polymorphic with 2-5 haplotypes differing by 1-5 mutations.…”