Nest Survival in Painted Stork (<i>Mycteria leucocephala</i>) Colonies of North India: the Significance of Nest Age, Annual Rainfall and Winter Temperature

Tiwary, Nawin Kumar; Urfi, Abdul Jamil

doi:10.1675/063.039.0205

Cited by 4 publications

(1 citation statement)

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“…Although the assignment test and differentiation results of our study indicate a lack of genetic structuring, due to low number of loci used, the presence of similar genotypes between the nesting seasons is not a strong reason to conclude that it is indeed the same individuals which are visiting the zoo year after year. Field observations indicate that in any given season PS can fluctuate between the short distance separated colonies [ 28 ]. So to be more certain if PS display nesting site fidelity, there is a need to test the genetic connectivity of multiple, spatially and temporally distributed PS colonies.…”

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confidence: 99%

A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source

2017

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ObjectiveIn continuation of an earlier study in which we reported the cross-amplification of Wood stork microsatellites on the DNA obtained from molted feathers of Painted stork (Mycteria leucocephala), here we investigated the nature of cross-amplified microsatellites and the effect of non-invasive samples on cross-amplification success. In a limited manner, we also addressed the genetic diversity and differentiation in a north Indian population of the Painted Stork examined over three nesting seasons.ResultsAmong the nine cross-amplified loci, only 5 were polymorphic. Three and 6 loci exhibited low (< 50%) and high amplification success rates (> 80), respectively. For 36 of 145 samples most of the loci failed to amplify. For genetic diversity, only 3 loci could be used since others exhibited low amplification and linkage disequilibrium. Probability of identity (0.034) was not low enough to develop a confidence that the similar genotypes originate from the same individual. Forty-two unique genotypes were identified. In 3 loci, a low to moderate level of genetic diversity (mean He = 0.435) was reported. Non-significant Fst (0.003, P = 0.230), G’stH (0.005, P = 0.247) and Dest (0.003, P = 0.250) values indicate a lack of structuring in temporally distributed populations of Delhi Zoo. The limitations and uniqueness of this study are discussed.

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confidence: 99%