Nesfatin-1 and caspase-cleaved cytokeratin-18: Promising biomarkers for Alzheimer’s disease?

Alpua, Murat; Kısa, Üçler

doi:10.4149/bll_2019_046

Cited by 4 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen et al, 2019; Ge et al, 2015). Higher plasma level of nesfatin-1 was also reported in AD or diabetes mellitus patients and animal models (Alpua & Kisa, 2019) (Dong et al, 2013), with significant correlation to the cognitive dysfunction. In the present study, our results showed that the serum nesfatin-1 concentration was increased in the bilaterally hippocampal STZ-injected mice.Although the mechanism remains unclear, this results provide new evidence that nesfatin-1 might be involved with the insulin metabolism disorder-associated cognitive impairment in AD.…”

Section: Discussionmentioning

confidence: 81%

Impaired learning and memory ability induced by a bilaterally hippocampal injection of streptozotocin in mice: involved with the adaptive changes of synaptic plasticity

Chen

Gao

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundAlzheimer’s disease (AD) is a neurodegenerative disease characterized by progressive cognitive decline, psychiatric symptoms and behavioral disorders, resulting in disability and loss of self-sufficiency.ObjectiveTo establish an AD mice model, investigate the behavioral performance, and explore the potential mechanism.MethodsStreptozotocin (STZ, 3 mg/kg) was microinjected bilaterally into the dorsal hippocampus of C57BL /6 mice to establish the AD model. Behavioral changes (anhedonia and despair, balance and motor coordination, locomotion, and learning and memory) were examined and the serum concentrations of insulin and nesfatin-1 were measured by ELISA. The activation of hippocampal microglia was assessed by immunohistochemistry and the protein expression of several molecular associated with the regulation of synaptic plasticity in the hippocampus and the prefrontal cortex (PFC) was detected via western blotting.ResultsThe STZ model mice showed a slower bodyweight gain and higher serum concentration of insulin and nesfatin-1. Although there was no significant difference between groups with regard to the ability of balance and motor coordination, the model mice presented a decline of spontaneous movement and exploratory behavior, together with an impairment of learning and memory ability. Increased activated microglia was aggregated in the hippocampal dentate gyrus of model mice. Moreover, the protein expression of NMDAR2A, NMDAR2B, SynGAP, PSD95, BDNF, and p-β-catenin/β-catenin were remarkably decreased in the hippocampus and the PFC of model mice, and the expression of p-GSK-3β (ser9)/GSK-3β were reduced in the hippocampus.ConclusionA bilateral hippocampal microinjection of STZ could successfully duplicate an AD mice model, as indicated by the impaired learning and memory and the alternated synaptic plasticity, together with the hyperactive inflammatory response in the hippocampus and the imbalanced abundance of serum insulin and nesfatin-1. Apart from these, the mechanism might be associated with the imbalanced expression of the key proteins of Wnt signaling pathway in the hippocampus and the PFC.Graphical abstract

show abstract

Section: Discussionmentioning

confidence: 81%

Impaired learning and memory ability induced by a bilaterally hippocampal injection of streptozotocin in mice: involved with the adaptive changes of synaptic plasticity

Chen

Gao

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Results of our previous study demonstrated that peripheral abundance of nesfatin-1 could be changed by high-fat diet and chronic stress, and ip injection of nesfatin-1 could induce anxiety and depressionlike behavioral disorder in rats, together with the change of hippocampal synaptic plasticity (Ge et al, 2015;Chen et al, 2019). Higher plasma level of nesfatin-1 was also reported in AD or diabetes mellitus patients and animal models (Dong et al, 2013;Alpua and Kisa, 2019), with significant correlation to the cognitive dysfunction. In the present study, our results showed that the serum nesfatin-1 concentration was increased in the bilaterally hippocampal STZ-injected mice.…”

Section: Discussionmentioning

confidence: 95%

Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity

Chen

Gao

et al. 2021

Front. Aging Neurosci.

View full text Add to dashboard Cite

Background: Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive cognitive decline, psychiatric symptoms and behavioral disorders, resulting in disability, and loss of self-sufficiency.Objective: To establish an AD-like mice model, investigate the behavioral performance, and explore the potential mechanism.Methods: Streptozotocin (STZ, 3 mg/kg) was microinjected bilaterally into the dorsal hippocampus of C57BL/6 mice, and the behavioral performance was observed. The serum concentrations of insulin and nesfatin-1 were measured by ELISA, and the activation of hippocampal microglia and astrocytes was assessed by immunohistochemistry. The protein expression of several molecular associated with the regulation of synaptic plasticity in the hippocampus and the pre-frontal cortex (PFC) was detected via western blotting.Results: The STZ-microinjected model mice showed a slower bodyweight gain and higher serum concentration of insulin and nesfatin-1. Although there was no significant difference between groups with regard to the ability of balance and motor coordination, the model mice presented a decline of spontaneous movement and exploratory behavior, together with an impairment of learning and memory ability. Increased activated microglia was aggregated in the hippocampal dentate gyrus of model mice, together with an increase abundance of Aβ1−42 and Tau in the hippocampus and PFC. Moreover, the protein expression of NMDAR2A, NMDAR2B, SynGAP, PSD95, BDNF, and p-β-catenin/β-catenin were remarkably decreased in the hippocampus and the PFC of model mice, and the expression of p-GSK-3β (ser9)/GSK-3β were reduced in the hippocampus.Conclusion: A bilateral hippocampal microinjection of STZ could induce not only AD-like behavioral performance in mice, but also adaptive changes of synaptic plasticity against neuroinflammatory and endocrinal injuries. The underlying mechanisms might be associated with the imbalanced expression of the key proteins of Wnt signaling pathway in the hippocampus and the PFC.

show abstract

“…Currently, little is known about the role of KRT18 in VD. However, according to a recent study (Alpua and Kisa 2019), caspase-cleaved keratin 18 (CCCK-18) can be used as a prognostic biomarker for Alzheimer's disease. Further comprehensive studies should be performed to clarify the use of CCCK-18 levels as a biomarker for other neuronal diseases.…”

Section: Discussionmentioning

confidence: 99%

Marinoid J, a phenylglycoside from Avicennia marina fruit, ameliorates cognitive impairment in rat vascular dementia: a quantitative iTRAQ proteomic study

Tang

et al. 2020

Pharmaceutical Biology

View full text Add to dashboard Cite

Nesfatin-1 and caspase-cleaved cytokeratin-18: Promising biomarkers for Alzheimer’s disease?

Cited by 4 publications

References 22 publications

Impaired learning and memory ability induced by a bilaterally hippocampal injection of streptozotocin in mice: involved with the adaptive changes of synaptic plasticity

Impaired learning and memory ability induced by a bilaterally hippocampal injection of streptozotocin in mice: involved with the adaptive changes of synaptic plasticity

Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity

Marinoid J, a phenylglycoside from Avicennia marina fruit, ameliorates cognitive impairment in rat vascular dementia: a quantitative iTRAQ proteomic study

Contact Info

Product

Resources

About