Nervous system disruption and concomitant behavioral abnormality in early hatched pufferfish larvae exposed to heavy oil

Kawaguchi, Masahumi; Sugahara, Yuki; Watanabe, Tomoe; Irie, Kouta; Ishida, Masanori; Kurokawa, Daisuke; Kitamura, Shin-Ichi; Takata, Hiromi; Handoh, Itsuki C.; Nakayama, Kei; Murakami, Yasunori

doi:10.1007/s11356-012-0833-0

Cited by 23 publications

(15 citation statements)

References 34 publications

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“…; Kawaguchi et al . ). After the color development, specimens were dissected and isolated limbs were observed under a stereomicroscope (Carl Zeiss).…”

Section: Methodsmentioning

confidence: 97%

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Expression patterns of Sema3A in developing amniote limbs: With reference to the diversification of peripheral nerve innervation

et al. 2017

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Paired limbs were acquired in the ancestor of tetrapods and their morphology has been highly diversified in amniotes in relation to the adaptive radiation to the terrestrial environment. These morphological changes may have been induced by modification of the developmental program of the skeletal or muscular system. To complete limb modification, it is also important to change the neuronal framework, because the functions of the limbs rely on neural circuits that involve coordinated movement. Previous studies have shown that class 3 semaphorins (Sema3 semaphorins), which act as repulsive axonal guidance cues, play a crucial role in the formation of the peripheral nerves in mice. Here, we studied the expression pattern of Sema3A orthologues in embryos of developing amniotes, including mouse, chick, soft-shelled turtle, and ocelot gecko. Sema3A transcripts were expressed in restricted mesenchymal parts of the developing limb primordium in all animals studied, and developing spinal nerves appeared to extend through Sema3A-negative regions. These results suggest that a Sema3A-dependent guidance system plays a key role in neuronal circuit formation in amniote limbs. We also found that Sema3A partially overlapped with the distribution of cartilage precursor cells. Based on these results, we propose a model in which axon guidance and skeletogenesis are linked by Sema3A; such mechanisms may underlie functional neuron rearrangement during limb diversification.

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“…; Kawaguchi et al . ). After the color development, specimens were dissected and isolated limbs were observed under a stereomicroscope (Carl Zeiss).…”

Section: Methodsmentioning

confidence: 97%

“…In situ hybridization was performed as described previously (Takio et al 2007;Kawaguchi et al 2011). After the color development, specimens were dissected and isolated limbs were observed under a stereomicroscope (Carl Zeiss).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Expression patterns of Sema3A in developing amniote limbs: With reference to the diversification of peripheral nerve innervation

et al. 2017

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“…Behavioral analyses at the early larval stage (stage 46–47) were performed as described previously [ 38 ], with minor modifications (Fig. 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The swimming trajectory was visualized on a personal computer, and the swimming speed and distance were measured using the Move-tr/2D7.0 software (Library Co. Ltd). To calculate the swimming pattern, the maximum and the minimum values of the swimming trajectory in both the x- and the y-axis were obtained as the swimming area (see also [ 38 ]). The whole experiment was performed twice.…”

Section: Methodsmentioning

confidence: 99%

Involvement of Slit–Robo signaling in the development of the posterior commissure and concomitant swimming behavior in Xenopus laevis

et al. 2015

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IntroductionDuring vertebrate development, the central nervous system (CNS) has stereotyped neuronal tracts (scaffolds) that include longitudinal and commissural axonal bundles, such as the medial longitudinal fascicle or the posterior commissure (PC). As these early tracts appear to guide later-developing neurons, they are thought to provide the basic framework of vertebrate neuronal circuitry. The proper construction of these neuronal circuits is thought to be a crucial step for eliciting coordinated behaviors, as these circuits transmit sensory information to the integrative center, which produces motor commands for the effective apparatus. However, the developmental plan underlying some commissures and the evolutionary transitions they have undergone remain to be elucidated. Little is known about the role of axon guidance molecules in the elicitation of early-hatched larval behavior as well.ResultsHere, we report the developmentally regulated expression pattern of axon-guidance molecules Slit2 ligand and Robo2 receptor in Xenopus laevis and show that treatment of X. laevis larvae with a slit2- or robo2-morpholino resulted in abnormal swimming behavior. We also observed an abnormal morphology of the PC, which is part of the early axonal scaffold.ConclusionOur present findings suggest that expression patterns of Slit2 and Robo2 are conserved in tetrapods, and that their signaling contributes to the construction of the PC in Xenopus. Given that the PC also includes several types of neurons stemming from various parts of the CNS, it may represent a candidate prerequisite neuronal tract in the construction of subsequent complex neuronal circuits that trigger coordinated behavior.

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“…To examine toxicological effects of heavy oil (HO, oil with high viscosity and density) Kawaguchi et al [51] performed embryonic exposure experiments and morphological and behavioral analyses in pufferfish (Takifugu rubripes) larvae. Fertilized eggs were exposed to 50 mg• L −1 of HO for 8 days then transferred to clean seawater before hatching.…”

Section: Oil and Hydrocarbonsmentioning

confidence: 99%

Delayed Behavioral Effects of Early Life Toxicant Exposures in Aquatic Biota

Weis

2014

Toxics

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Behavioral development occurs together with the development of the nervous system. Studies on mammals indicate that exposures to some chemicals during embryonic development at concentrations that do not produce anatomical malformations may nevertheless produce behavioral deficits later in life, an example of delayed effects. There have been reports of delayed effects in aquatic organisms. Delayed behavioral effects of mercury, chlorinated and other pesticides, polycyclic aromatic hydrocarbons (PAHs), and some synthetic hormones in the environment have been reported in fishes and invertebrates; in some cases behavioral effects are manifested years after the exposure. Another type of delayed behavioral effect results from exposure of mature females before fertilization (maternal exposure). Even when embryos and larvae are reared in clean water, offspring may manifest abnormal behaviors following maternal exposure. The reported behavioral changes are generally deleterious and compromise the fitness of the animal in its natural environment. Delayed effects and their impacts on fitness are not considered in standard short-term embryo bioassays, which will therefore underestimate neurotoxicity. The literature in the field is scattered and has not been reviewed. The objective of this paper is to review and synthesize what is known about delayed behavioral effects in aquatic biota.

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Nervous system disruption and concomitant behavioral abnormality in early hatched pufferfish larvae exposed to heavy oil

Cited by 23 publications

References 34 publications

Expression patterns of Sema3A in developing amniote limbs: With reference to the diversification of peripheral nerve innervation

Expression patterns of Sema3A in developing amniote limbs: With reference to the diversification of peripheral nerve innervation

Involvement of Slit–Robo signaling in the development of the posterior commissure and concomitant swimming behavior in Xenopus laevis

Delayed Behavioral Effects of Early Life Toxicant Exposures in Aquatic Biota

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