Nerve–stem cell crosstalk in skin regeneration and diseases

Peng, Jingyu; Chen, Han; Zhang, Bing

doi:10.1016/j.molmed.2022.04.005

Cited by 10 publications

(4 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Burn injuries are devastating, affecting approximately 11 million people each year. , Besides the urgent need to quickly close wounds and help patients survive extensive burns, the reconstruction of skin appendages, such as sweat glands, is also important for the functional recovery of burn patients. − Recent studies have emphasized the significance of cutaneous nerves in maintaining skin physiology and supporting appendages. , Without proper innervation, wound healing can be compromised, leading to poor outcomes . Current burn treatments, such as autografts, allografts, artificial skin grafts, and cell-based replacement therapies, mainly focus on introducing external substances to expedite wound coverage but are insufficient to regenerate a physiological-like niche infiltrated by nerves and vascular network .…”

Section: Introductionmentioning

confidence: 99%

“…3−5 Recent studies have emphasized the significance of cutaneous nerves in maintaining skin physiology and supporting appendages. 6,7 Without proper innervation, wound healing can be compromised, leading to poor outcomes. 8 Current burn treatments, such as autografts, allografts, artificial skin grafts, and cell-based replacement therapies, mainly focus on introducing external substances to expedite wound coverage but are insufficient to regenerate a physiological-like niche infiltrated by nerves and vascular network.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Flexible Sono–Piezo Patch for Functional Sweat Gland Repair through Endogenous Microenvironmental Remodeling

Pi,

Chen,

Liu

et al. 2024

ACS Nano

View full text Add to dashboard Cite

Remodeling the endogenous regenerative microenvironment in wounds is crucial for achieving scarless, functional tissue regeneration, especially the functional recovery of skin appendages such as sweat glands in burn patients. However, current approaches mostly rely on the use of exogenous materials or chemicals to stimulate cell proliferation and migration, while the remodeling of a pro-regenerative microenvironment remains challenging. Herein, we developed a flexible sono−piezo patch (fSPP) that aims to create an endogenous regenerative microenvironment to promote the repair of sweat glands in burn wounds. This patch, composed of multifunctional fibers with embedded piezoelectric nanoparticles, utilized low-intensity pulsed ultrasound (LIPUS) to activate electrical stimulation of the target tissue, resulting in enhanced pro-regenerative behaviors of niche tissues and cells, including peripheral nerves, fibroblasts, and vasculatures. We further demonstrated the effective wound healing and regeneration of functional sweat glands in burn injuries solely through such physical stimulation. This noninvasive and drug-free therapeutic approach holds significant potential for the clinical treatment of burn injuries.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flexible Sono–Piezo Patch for Functional Sweat Gland Repair through Endogenous Microenvironmental Remodeling

Pi,

Chen,

Liu

et al. 2024

ACS Nano

View full text Add to dashboard Cite

show abstract

“…1a). Other skin structures, such as nerves and sub-cutaneous muscles, may also be remodeled during the hair cycle 16 , but this is not well understood. Characterization of the cellular heterogeneity of skin VE-cadherin + lineage organization and the changes in molecular makeup as this lineage remodels during adult skin homeostasis is currently lacking.…”

Section: Introductionmentioning

confidence: 99%

Single-cell transcriptomics of adult skin VE-cadherin expressing lineages during hair cycle

Chovatiya

Ghuwalewala

et al. 2023

Preprint

View full text Add to dashboard Cite

Adult skin homeostasis involves global reorganization of dermal lineages at different stages of the mouse hair growth cycle. Vascular endothelial cadherin (VE-cadherin encoded by Cdh5) expressing cells from blood and lymphatic vasculature structures are known to remodel during the adult hair cycle. Here we employ single-cell RNA-sequencing (scRNA-seq) 10x-genomics analysis of FACS-sorted VE-cadherin expressing cells marked via Cdh5-CreER genetic labeling at resting (telogen) and growth (anagen) stage of hair cycle. Our comparative analysis between the two stages uncovers a persistent Ki67+ proliferative EC population and documents changes in EC population distribution and gene expression. Global gene expression changes in all the analyzed populations revealed bioenergetic metabolic changes that may drive vascular remodeling during HF growth phase, alongside a few highly restricted cluster-specific gene expression differences. This study uncovers active cellular and molecular dynamics of adult skin endothelial lineages during hair cycle that may have broad implications in adult tissue regeneration and for understanding vascular disease.

show abstract

“…Alk1, the known VE-cadherin interacting partner, acts in all skin ECs to regulate their migration and the vasculature remodeling during hair cycle 16 . Other skin structures, such as nerves and sub-cutaneous muscles, may also be remodeled during the hair cycle 17 . A thorough characterization of the cellular and functional heterogeneity of skin VE-cadherin + lineage organization during adult skin homeostasis is currently lacking.…”

Section: Introductionmentioning

confidence: 99%

Alk1 acts in non-endothelial VE-cadherin+ perineurial cells to maintain nerve branching during hair homeostasis

Chovatiya,

Li,

et al. 2023

Nat Commun

View full text Add to dashboard Cite

Vascular endothelial (VE)-cadherin is a well-recognized endothelial cell marker. One of its interacting partners, the TGF-β receptor Alk1, is essential in endothelial cells for adult skin vasculature remodeling during hair homeostasis. Using single-cell transcriptomics, lineage tracing and gene targeting in mice, we characterize the cellular and molecular dynamics of skin VE-cadherin+ cells during hair homeostasis. We describe dynamic changes of VE-cadherin+ endothelial cells specific to blood and lymphatic vessels and uncover an atypical VE-cadherin+ cell population. The latter is not a predicted adult endovascular progenitor, but rather a non-endothelial mesenchymal perineurial cell type, which forms nerve encapsulating tubular structures that undergo remodeling during hair homeostasis. Alk1 acts in the VE-cadherin+ perineurial cells to maintain proper homeostatic nerve branching by enforcing basement membrane and extracellular matrix molecular signatures. Our work implicates the VE-cadherin/Alk1 duo, classically known as endothelial-vascular specific, in perineurial-nerve homeostasis. This has broad implications in vascular and nerve disease.

show abstract

Nerve–stem cell crosstalk in skin regeneration and diseases

Cited by 10 publications

References 96 publications

Flexible Sono–Piezo Patch for Functional Sweat Gland Repair through Endogenous Microenvironmental Remodeling

Flexible Sono–Piezo Patch for Functional Sweat Gland Repair through Endogenous Microenvironmental Remodeling

Single-cell transcriptomics of adult skin VE-cadherin expressing lineages during hair cycle

Alk1 acts in non-endothelial VE-cadherin+ perineurial cells to maintain nerve branching during hair homeostasis

Contact Info

Product

Resources

About