Nerve regeneration with aid of nanotechnology and cellular engineering

Sedaghati, Tina; Yang, Shi Yu; Mosahebi, Afshin; Alavijeh, Mohammad S.; Seifalian, Alexander M.

doi:10.1002/bab.51

Cited by 30 publications

(18 citation statements)

References 144 publications

(129 reference statements)

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“…The field of bioengineering nerve conduits for regenerative medicine is advancing rapidly [102,103]. Despite the tremendous amount of research conducted in the search for appropriate materials for nerve conduits, the FDA currently approves only a few materials for that purpose, namely PGA, Type-I collagen, PLCL, and PVA.…”

Section: Discussionmentioning

confidence: 99%

Biochemical engineering nerve conduits using peptide amphiphiles

Tan

Rajadas

Seifalian

2012

Journal of Controlled Release

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Peripheral nerve injury is a debilitating condition. The gold standard for treatment is surgery, requiring an autologous nerve graft. Grafts are harvested from another part of the body (a secondary site) to treat the affected primary area. However, autologous nerve graft harvesting is not without risks, with associated problems including injury to the secondary site. Research into biomaterials has engendered the use of bioartificial nerve conduits as an alternative to autologous nerve grafts. These include synthetic and artificial materials, which can be manufactured into nerve conduits using techniques inspired by nanotechnology. Recent evidence indicates that peptide amphiphiles (PAs) are promising candidates for use as materials for bioengineering nerve conduits. PAs are biocompatible and biodegradable protein-based nanomaterials, capable of self-assembly in aqueous solutions. Their self-assembly system, coupled with their intrinsic capacity for carrying bioactive epitopes for tissue regeneration, form particularly novel attributes for biochemically-engineered materials. Furthermore, PAs can function as biomimetic materials and advanced drug delivery platforms for sustained and controlled release of a plethora of therapeutic agents. Here we review the realm of nerve conduit tissue engineering and the potential for PAs as viable materials in this exciting and rapidly advancing field.

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Section: Discussionmentioning

confidence: 99%

Biochemical engineering nerve conduits using peptide amphiphiles

Tan

Rajadas

Seifalian

2012

Journal of Controlled Release

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“…[19] Currently, researchers are designing neural interfaces between the peripheral and central nervous system, and limb prostheses for patients with traumatic amputations and spinal cord injuries. [24,25] Figure 1: Histological staining of healed skin in each experimental group. Masson trichome staining shows the distribution and density of collagen protein in healed skin in various groups.…”

Section: Nerve Tubulizationmentioning

confidence: 99%

Current and future applications of nanotechnology in plastic and reconstructive surgery

Petersen¹,

Naylor²,

Halen³

2014

Plast Aesthet Res

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“…Thus, the number, arrangement and density of axons are very important factors to evaluate nerve regeneration. 23,24 At 4 weeks after surgery the nerve specimens were crosssectioned, stained with toluidine blue and observed in a light microscope ( Figure 9). The nerve specimens were also made into ultrathin sections for transmission electron microscopy (TEM) observation (Figure 10).…”

Section: Histological Observationsmentioning

confidence: 99%

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Wei¹,

Yao²,

Wang³

et al. 2013

IJN

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Background: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk's biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury. Methods: The SF16 peptide's structure was characterized using spectrophotometry and atomic force microscopy, and the SF16 hydrogel was analyzed using scanning electron microscopy. The effects of the SF16 hydrogel on the viability and growth of live cells was first assessed in vitro, on PC12 cells. The in vivo test model involved the repair of a nerve gap with tubular nerve guides, through which it was possible to identify if the SF16 hydrogel would have the potential to enhance nerve regeneration. In this model physiological saline was set as the negative control, and collagen as the positive control. Walking track analysis and electrophysiological methods were used to evaluate the functional recovery of the nerve at 4 and 8 weeks after surgery. Results: Analysis of the SF16 peptide's characteristics indicated that it consisted of a welldefined secondary structure and exhibited self-assembly. Results of scanning electron microscopy showed that the peptide based hydrogel may represent a porous scaffold that is viable for repair of peripheral nerve injury. Analysis of cell culture also supported that the hydrogel was an effective matrix to maintain the viability, morphology and proliferation of PC12 cells. Electrophysiology demonstrated that the use of the hydrogel scaffold (SF16 or collagen) resulted in a significant improvement in amplitude recovery in the in vivo model compared to physiological saline. Moreover, nerve cells in the SF16 hydrogel group displayed greater axon density, larger average axon diameter and thicker myelin compared to those of the group that received physiological saline. Conclusion: The SF16 hydrogel scaffold may promote excellent axonal regeneration and functional recovery after peripheral nerve injury, and the SF16 peptide may be a candidate for nerve tissue engineering applications.

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Nerve regeneration with aid of nanotechnology and cellular engineering

Cited by 30 publications

References 144 publications

Biochemical engineering nerve conduits using peptide amphiphiles

Biochemical engineering nerve conduits using peptide amphiphiles

Current and future applications of nanotechnology in plastic and reconstructive surgery

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

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