Nerve regeneration over a 25 mm gap in rat sciatic nerves using tubes containing blood vessels: the possibility of clinical application

Kakinoki, Ryosuke; Nishijima, Norihiro; Ueba, Yasuyuki; Oka, M.; Yamamuro, Takao; Nakamura, Tatsuo

doi:10.1007/s002640050179

Cited by 26 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Autologous nerve grafting remains the gold standard for reconstruction of long and/or complex peripheral nerve injuries, but the use of autologous nerve grafts is potentially complicated by the limited amount of available nerve graft material and the potential for donor site morbidity. Recently, several surgical alternatives, including the use of acellular nerve allograft, 18 decellularized artery allograft, 19 vein graft, 20 tubes containing blood vessels, 21 and synthetic or biologic nerve conduits, 19,22 have been reported with various success. However, these methods of nerve reconstruction are less effective when the proximal stump of an injured nerve is unavailable or the nerve gap is too long to be effectively bridged by a nerve graft.…”

Section: Discussionmentioning

confidence: 99%

Changes of the donor nerve in end‐to‐side neurorrhaphies with epineurial window and partial neurectomy: A long‐term evaluation in the rat model

et al. 2013

View full text Add to dashboard Cite

End-to-side (ETS) neurorrhaphy has been applied in the repair of peripheral nerve injuries and in babysitter procedures. However, the long-term changes of donor nerve and muscle after ETS remain unknown. This study was designed to investigate long-term changes in donor nerve and muscle in a rat model. Sixty Lewis rats were equally allocated into three groups of 20 rats. The peroneal nerve was divided. In Group A, end-to-end (ETE) neurorrhaphy was performed. In Group B, ETS was performed to an epineurial window on the tibial nerve. In Group C, ETS was performed to the tibial nerve with 40% partial neurectomy. The following data were obtained at 6, 12, 18, and 24 weeks postoperatively: latency delaying rate (LDR), amplitude recovery rate (ARR), myelinated fiber counts, muscle force and weight, and cross-sectional area of gastrocnemius muscle fibers. The results showed no significant changes of the donor nerve and muscle in Group B. Nerve regeneration was found in the peroneal nerve, and myelinated fiber number was significantly decreased when compared to the nerve with ETE. In Group C, the myelinated axon number in the peroneal nerve was equivalent to the level in ETE repair. However, function and structure of the donor nerve and muscle were significantly impaired in the early postoperative period. Nonetheless, full recovery was observed 24 weeks after surgery. Both ETS with epineurial window and 40% donor nerve neurectomy showed reinnervation of the recipient nerve without structural and functional changes of the donor system in a long-term follow-up. Partial neurectomy may promote recipient nerve regeneration, but at the cost of donor neuromuscular compromises in the early postoperative period. This study provides long-term evidence for further investigation of ETS in peripheral nerve repair and in babysitter procedures.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes of the donor nerve in end‐to‐side neurorrhaphies with epineurial window and partial neurectomy: A long‐term evaluation in the rat model

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Numerous investigators have studied regeneration across extended gap lesions (longer than 1.4 cm) in the rat model (Williams et al, 1987; Yannas et al, 1987; Madison et al, 1988; Kakinoki et al, 1997, 1998; Hadlock et al, 1998; Arai et al, 2000). To our knowledge, none had reported regeneration across these gap distances with empty tube controls.…”

Section: Discussionmentioning

confidence: 99%

Poly(L‐Lactide) microfilaments enhance peripheral nerve regeneration across extended nerve lesions

Ngo

Waggoner

Romero

et al. 2003

J of Neuroscience Research

136

View full text Add to dashboard Cite

After injury, axonal regeneration occurs across short gaps in the peripheral nervous system, but regeneration across larger gaps remains a challenge. To improve regeneration across extended nerve defects, we have fabricated novel microfilaments with the capability for drug release to support cellular migration and guide axonal growth across a lesion. In this study, we examine the nerve repair parameters of non-loaded filaments. To examine the influence of packing density on nerve repair, wet-spun poly(L-Lactide) (PLLA) microfilaments were bundled at densities of 3.75, 7.5, 15, and 30% to bridge a 1.0-cm gap lesion in the rat sciatic nerve. After 10 weeks, nerve cable formation increased significantly in the filament bundled groups when compared to empty-tube controls. At lower packing densities, the number of myelinated axons was more than twice that of controls or the highest packing density. In a consecutive experiment, PLLA bundles with lower filament-packing density were examined for nerve repair across 1.4- and 1.8-cm gaps. After 10 weeks, the number of successful regenerated nerves receiving filaments was more than twice that of controls. In addition, nerve cable areas for control groups were significantly less than those observed for filament groups. Axonal growth across 1.4- and 1.8-cm gaps was more consistent for the filament groups than for controls. These initial results demonstrate that PLLA microfilaments enhance nerve repair and regeneration across large nerve defects, even in the absence of drug release. Ongoing studies are examining nerve regeneration using microfilaments designed to release neurotrophins or cyclic AMP.

show abstract

“…Thus, materials such as collagen, arteries, veins, cell adhesion molecules, Polyethylene tubes were proposed (Kakinoki et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Ppe Tube Filled With Autologous Fat in the Repair of Peripheral Nerve: A New Proposal

et al. 2016

View full text Add to dashboard Cite

SUMMARY:This study proposes the use of a porous polyethylene (PPE) tube as the conductive element in the regeneration in the sciatic nerve sectioning and evaluates the use of fill with autologous fat. The subject was divided randomly into five groups, 3 control and 2 experimental (PPE tube graft with/ without autologous fat). Each group was selected for functional, histological and morphometric evaluation of the sciatic nerve. Functional analysis of the sciatic nerve occurred through the "footprint" values near -100 refer sectioned sciatic nerve, near 0 (zero) refer to control group. On histological analysis of the experimental groups lots of dense connective tissue replacing nerve tissue was observed. In morphometric analysis the group EGPGf got higher performance in all of variables. The use of PPE has shown promise in nerve regeneration with favorable results when associate with fat as a trophic factor in the regeneration.

show abstract

Nerve regeneration over a 25 mm gap in rat sciatic nerves using tubes containing blood vessels: the possibility of clinical application

Cited by 26 publications

References 31 publications

Changes of the donor nerve in end‐to‐side neurorrhaphies with epineurial window and partial neurectomy: A long‐term evaluation in the rat model

Changes of the donor nerve in end‐to‐side neurorrhaphies with epineurial window and partial neurectomy: A long‐term evaluation in the rat model

Poly(L‐Lactide) microfilaments enhance peripheral nerve regeneration across extended nerve lesions

Ppe Tube Filled With Autologous Fat in the Repair of Peripheral Nerve: A New Proposal

Contact Info

Product

Resources

About