2003

DOI: 10.1016/s0142-9612(02)00572-0

|View full text |Cite

|

Sign up to set email alerts

|

Nerve regeneration in a collagen–chitosan tissue-engineered skin transplanted on nude mice

Marie Claude Gingras

¹

,

Isabelle Paradis

²

,

François Berthod

³

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

151

Discussion1

Fig 2: Sa-β-gal Positive Nih3t3 Mouse Fibro Blasts Cells (C1

Kitozanın Yumuşak Doku üZerine Iyileştirici Etkisi1

Citation Types

Supporting

2

Mentioning

79

Contrasting

0

Unclassified

2

Year Published

2011

2011

2021

2021

Publication Types

Select...

Article7

Other2

Book1

Relationship

Self Cite0

Independent10

Authors

Journals

Cited by 142 publications

(83 citation statements)

References 47 publications

Supporting

2

Mentioning

79

Contrasting

0

Unclassified

2

Order By: Relevance

“…Although Coolen et al (29) showed that keratinocytes can be cultured without a fibroblast feeder layer and fetal calf serum, the present results demonstrated that in the modified medium and onto a type I collagen scaffold (as a feeder layer), keratinocyte cell growth was greater than the condition without a feeder layer, and this is consistent with certain previous studies by Gingras et al (31) and Arpornmaeklong et al (32) in 2007. However, a long-term follow-up study is required to more precisely evaluate its fate following proper grafting.…”

Section: Discussionsupporting

confidence: 92%

Comparison of the enzymatic and explant methods for the culture of keratinocytes isolated from human foreskin

¹

,

²

,

³

et al. 2015

Biomedical Reports

View full text Add to dashboard Cite

Abstract. Currently, culture and growth keratinocytes are important stages in achieving a reliable and reproducible skin tissue. In the present study, two different methods, enzymatic and explant methods, for keratinocytes isolation from human foreskin were compared. Foreskins were cut into 2-3 mm pieces and placed in trypsin at 4˚C overnight for separation of the epidermis from the dermis. Subsequently, these samples were divided into two groups: i) Keratinocytes separated from the epidermis by trypsin and ii) by the explant method. These keratinocytes were divided into two groups: i) With no feeder layer and ii) onto a type I collagen scaffold. The cells were evaluated using immunocytochemistry and 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI) staining. In the enzymatic treatment, after 7-10 days no attached cells were found in the cell culture dishes. In the explant method, keratinocytes were separated after ~24 h, attached rapidly and formed big colonies into a collagen scaffold. In the absence of a feeder layer, small colonies were developed with rapid loss of proliferation within 2-3 days. Keratinocytes showed positive immunoreactivity for the pan-cytokeratin marker and keratinocytes' nuclei were clearly observed. This method could be applied and developed as a component of skin substitutes to treat burns and wounds and also in laboratory testing. IntroductionThe skin is the largest organ in the body that is divided into two anatomically distinct regions, the dermis and epidermis. The normal structure and function of this organ is dependent on the intact epidermis anchored to its vascular, elastic dermis (1,2). Fibroblasts are the most prevalent cell types in the dermis, which produce different growth factors that induce proliferation of keratinocytes in vivo and in vitro (3). The principal cell type of the epidermis is the keratinocyte (1,4), which is a small epithelial cell, located at the top of the epidermal basal membrane and characterized by a low division rate (5,6).Thus far, various enzymatic methods for dermal-epidermal separation have been applied (6-8). For instance, trypsin separates suprabasal hemidesmosomes that causes the basal layer cells to attach to the dermal layer (6). Thermolysin is another enzyme that selectively separates desmosomes (5,9) and is able to separate the epidermis at the basal membrane zone level (5,10).Although the dissociation method of keratinocytes in primary culture is well-established, attempts to acquire purified adult stem-cell like⁄progenitor keratinocytes from whole human skin are still ongoing. In particular, different techniques are currently being applied to achieve high purity or homogeneous primary cultures enriched in keratinocyte progenitor⁄precursor cells. These include filtration, density gradient centrifugation and fluorescence-activated cell sorting using cell surface antibodies, as well as differential adhesion to enrich the cells that rapidly attach to particular substrates (11).A previous study showed that when keratinocytes/precursor cells...

“…Although Coolen et al (29) showed that keratinocytes can be cultured without a fibroblast feeder layer and fetal calf serum, the present results demonstrated that in the modified medium and onto a type I collagen scaffold (as a feeder layer), keratinocyte cell growth was greater than the condition without a feeder layer, and this is consistent with certain previous studies by Gingras et al (31) and Arpornmaeklong et al (32) in 2007. However, a long-term follow-up study is required to more precisely evaluate its fate following proper grafting.…”

Section: Discussionsupporting

confidence: 92%

Comparison of the enzymatic and explant methods for the culture of keratinocytes isolated from human foreskin

¹

,

²

,

³

et al. 2015

Biomedical Reports

View full text Add to dashboard Cite

Abstract. Currently, culture and growth keratinocytes are important stages in achieving a reliable and reproducible skin tissue. In the present study, two different methods, enzymatic and explant methods, for keratinocytes isolation from human foreskin were compared. Foreskins were cut into 2-3 mm pieces and placed in trypsin at 4˚C overnight for separation of the epidermis from the dermis. Subsequently, these samples were divided into two groups: i) Keratinocytes separated from the epidermis by trypsin and ii) by the explant method. These keratinocytes were divided into two groups: i) With no feeder layer and ii) onto a type I collagen scaffold. The cells were evaluated using immunocytochemistry and 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI) staining. In the enzymatic treatment, after 7-10 days no attached cells were found in the cell culture dishes. In the explant method, keratinocytes were separated after ~24 h, attached rapidly and formed big colonies into a collagen scaffold. In the absence of a feeder layer, small colonies were developed with rapid loss of proliferation within 2-3 days. Keratinocytes showed positive immunoreactivity for the pan-cytokeratin marker and keratinocytes' nuclei were clearly observed. This method could be applied and developed as a component of skin substitutes to treat burns and wounds and also in laboratory testing. IntroductionThe skin is the largest organ in the body that is divided into two anatomically distinct regions, the dermis and epidermis. The normal structure and function of this organ is dependent on the intact epidermis anchored to its vascular, elastic dermis (1,2). Fibroblasts are the most prevalent cell types in the dermis, which produce different growth factors that induce proliferation of keratinocytes in vivo and in vitro (3). The principal cell type of the epidermis is the keratinocyte (1,4), which is a small epithelial cell, located at the top of the epidermal basal membrane and characterized by a low division rate (5,6).Thus far, various enzymatic methods for dermal-epidermal separation have been applied (6-8). For instance, trypsin separates suprabasal hemidesmosomes that causes the basal layer cells to attach to the dermal layer (6). Thermolysin is another enzyme that selectively separates desmosomes (5,9) and is able to separate the epidermis at the basal membrane zone level (5,10).Although the dissociation method of keratinocytes in primary culture is well-established, attempts to acquire purified adult stem-cell like⁄progenitor keratinocytes from whole human skin are still ongoing. In particular, different techniques are currently being applied to achieve high purity or homogeneous primary cultures enriched in keratinocyte progenitor⁄precursor cells. These include filtration, density gradient centrifugation and fluorescence-activated cell sorting using cell surface antibodies, as well as differential adhesion to enrich the cells that rapidly attach to particular substrates (11).A previous study showed that when keratinocytes/precursor cells...

“…Hence for rapid hydration and regeneration of skin, aloe is intended to be used in this formulation to make the skin smooth and elastic. Though many studies have shown the incompatibility of skin fibroblasts with chitosan, it has been reported that the addition of collagen facilitates the attachment and proliferation of skin fibroblasts [34][35][36][37] .…”

Section: Fig 2: Sa-β-gal Positive Nih3t3 Mouse Fibro Blasts Cells (Cmentioning

confidence: 99%

Untitled

2018

View full text Add to dashboard Cite

Photochemoprotection has nowadays become inevitable to combat aging. Exposure to Ultraviolet radiations accelerates skin aging which eventually culminates in wrinkles, laxity, dyspigmentation, roughness and dryness. Though many active synthetic topicals have been used since years, the present era of treating an aged skin has been diverted towards natural biomaterials as these synthetic topicals pose health and safety risk on human health. In an effort to produce a promising natural anti-aging agent in contemporary cosmetics, this study has been aimed to formulate an anti-aging gel by blending three biopolymers namely Collagen (COL) 3%w/v, Chitosan (CS) 1.5% w/v and Aloe vera (AV) gel 0.21% w/v. The AV blended COL-CS gel was characterized by spreadability and moisture uptake test, which indicated good spreadability and increased hydrophyllicity of the gel. Cell culture studies on NIH3T3 mouse fibroblasts cells were carried out using senescenceassociated-β-gal [SA-β-gal] as a biomarker. The fibroblasts cells on incubation with AV blended COL-CS gel showed increased proliferation rate and the absence of blue stain indicated that the process of senesce has been reversed. In conclusion, the prepared AV blended COL-CS gel helps in regeneration and rejuvenation of the skin and therefore can be used as a promising anti-aging gel.

“…Kitozanın mukoza, deri, kıkırdak, sinir, retina, ürogenital, periodontal, kas ve tendon yaralanmalarında herhangi bir ters etki oluşturmaksızın iyileşmeyi hızlandırıcı etkileri olduğu belirlenmiştir. [42][43][44][45][46][47][48][49] Kitozanla iyileşen yaralarda yeni oluşan kollagen miktarı önemli ölçüde fazla olup özellikle tip III kollagende artışlar görülmüştür. Bu da, kitozanın granülasyon fazının gelişimine destek olduğu anlamına gelmektedir.…”

Section: Kitozanın Yumuşak Doku üZerine Iyileştirici Etkisiunclassified

Di̇ş Heki̇mli̇ği̇nde Ki̇ti̇n Ve Ki̇tozan

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

View full text Add to dashboard Cite

No abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Product

Browser Extension Assistant by scite Citation Statement Search Reference Check Visualizations Dashboards Explore Journals Explore Organizations Explore Funders Embedding Badge Embedding Citation Search Pricing

Resources

Blog Help & FAQ Accessibility Statement API Terms For Universities & Governments For Researchers For Publishers For Corporate, Pharma & Enterprise Author Marketing Become an Affiliate Get an organization trial or quote scite Data & Services

About

News & Press Careers Read our Paper Coverage

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Copyright © 2024 scite LLC. All rights reserved.

Made with 💙 for researchers

Part of the Research Solutions Family.