Nerve induced responses and force–velocity relations of regenerated detrusor muscle after subtotal cystectomy in the rat

Abstract

(1): A regional difference exists in pharmacological properties of control detrusor, with a considerable contractile response to stimulation remaining in the supratrigonal muscle after simultaneous cholinergic, adrenergic, and purinergic blockade. (2): The new detrusor was functionally well innervated with no supersensitivity to muscarinic stimulation. (3): The newly formed bladder body had pharmacological properties specific for the supratrigonal segment from which it had developed. (4): There was no regional difference in force-velocity characteristics of the control detrusor. (5): The lowered Vmax in the newly formed bladder might thus be related to growth and regeneration of muscle cells.

The Pharmacology of Regenerative Medicine

“…In addition, the presence of a response to electrical field stimulation indicated innervation of newly formed tissue. This 1108 agrees with an earlier study by Frederiksen et al (2004) in which whole mount staining of acetylcholinesterase revealed the pattern of nerves in newly formed detrusor.…”

“…The idea that the bladder tissue formed spontaneously after cystectomy is similar to that which remains was proposed by Frederiksen et al (2004) who described the pharmacology of regenerating bladder. Fifteen weeks after STC in female rats, transverse strips were excised from the bladder body and were exposed to contractile stimuli, taking into account the proximity of the strip to the trigone.…”

“…In pioneering studies by Atala et al (2006), bladder neo-organs were constructed by seeding synthetic scaffolds (collagen or collagen/polyglycolic acid composites) with urothelial cells on the inside and smooth muscle cells on the outside and subsequently implanted into subjects with myelomeningocele. Numerous reports suggested that both animal (Liang and Goss, 1963;Oberpenning et al, 1999;Frederiksen et al, 2004;Burmeister et al, 2010) and human (Sisk and Neu, 1939;Liang, 1962;Tucci and Haralambidis, 1963) bladders have significant regenerative potential after removal of a large portion of the organ (subtotal cystectomy; STC).…”

Nerve distribution in rat urinary bladder after incorporation of acellular matrix graft or subtotal cystectomy

“…The regeneration of the new bladder is to some extent attained by distension of the original base of the bladder. This is more pronounced in the rabbit [25] than the rat [26] bladder. Interestingly, the newly developed detrusor retains some of the pharmacological properties specific for the bladder base and this is more evident in the rabbit [25] than the rat [26] bladder.…”

“…This is more pronounced in the rabbit [25] than the rat [26] bladder. Interestingly, the newly developed detrusor retains some of the pharmacological properties specific for the bladder base and this is more evident in the rabbit [25] than the rat [26] bladder. The distribution of the nerve trunks in the new detrusor has not been studied until now.…”