Nerve-induced release of nitric oxide from the rabbit corpus cavernosum is modulated by cyclic guanosine 3′,5′-monophosphate

Hallén, Katarina; Gustafsson, Lars E.; Wiklund, Peter

doi:10.1016/j.neuroscience.2005.02.015

Cited by 10 publications

(16 citation statements)

References 36 publications

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“…This hypothesis also explains the inhibition on neuronal‐evoked responses because primarily eNOS is responsible for basal NO production in corpus cavernosum. In a previous study tetrodotoxin was shown not to alter basal NO levels in rabbit corpus cavernosum [39] indicating that nNOS does not contribute to basal NO production in this tissue. Acetylcholine mediated relaxant responses became sensitive to inhibition by LY 83583 administration following DETCA pretreatment indicating that higher levels of oxidant stress blocks the masking action of basal NO over the inhibition on acetylcholine‐induced relaxations.…”

Section: Discussionmentioning

confidence: 95%

Protective effect of quercetin, a polyphenolic compound, on mouse corpus cavernosum

Ertuğ¹,

Olguner

Ogulener

et al. 2010

Fundamemntal Clinical Pharma

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Flavonoids are plant-based phenolic compounds, and quercetin is the most abundant dietary member of this family. One of the most important characteristics of quercetin is its antioxidant property. The aim of this study was to investigate antioxidant effects of quercetin on corpora cavernosa of mice. Corpora cavernosa were isolated in organ baths, precontracted with phenylephrine (0.5 microm) and relaxant responses were mediated by acetylcholine (0.1-1 microm), electrical field stimulation (EFS, 1-16 Hz, 0.5 ms, 30 V) or acidified sodium nitrite (a NaNO(2), 0.5 mm). Superoxide anion generators; pyrogallol (50 microm), hydroquinone (100 microm), LY 83583 (6-Anilinoquinolin-5,8-quinone, 10 microm) and superoxide dismutase (SOD) inhibitor; diethyldithiocarbamic acid (DETCA, 8 mm) were used in order to expose corpus cavernosa to oxidant stress. Acetylcholine (0.1-1 microm) induced relaxant responses were significantly inhibited in LY 83583 (10 microm) and DETCA + LY 83583 applicated trials. EFS-induced relaxant responses were significantly inhibited in DETCA (8 mm) and DETCA + LY 83583 administrated trials. On the other hand, acidified sodium nitrite-induced responses were inhibited by all of the superoxide anion generators tested. Quercetin (10 microm) failed to improve the inhibitions on endothelium and electrically stimulated responses. Acidified sodium nitrite (0.5 mm) mediated relaxant responses were significantly restored by quercetin except the groups in which LY 83583 were used. The data suggest that quercetin acts as a protective agent in mouse corpus cavernosum, increasing the bioavailability of exogenous nitric oxide by protecting it from superoxide anion (O(2)(-)).

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Section: Discussionmentioning

confidence: 95%

Protective effect of quercetin, a polyphenolic compound, on mouse corpus cavernosum

Ertuğ¹,

Olguner

Ogulener

et al. 2010

Fundamemntal Clinical Pharma

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“…Previously, our group has quantified the release of NO from autonomic neurons of rabbit corpus cavernosum. The release of NO decreased in presence of the NOS inhibitor N w ‐nitro‐ L ‐arginine methyl ester ( L ‐NAME) and originated from neurons as indicated by the decreased release in the presence of tetrodotoxin (TTX) ( Hallén et al ., 2005 ).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, it has been described that cGMP might affect the release of NO from the autonomic neurons in guinea‐pig colon ( Hallén et al ., 2001 ) and in rabbit corpus cavernosum ( Hallén et al ., 2005 ). Application of modulators of the sGC/cGMP pathway (the analog 8‐Br‐cGMP, the sGC stimulator 3‐(5′‐hydroxymethyl‐2‐furyl)‐1‐benzylindazole (YC‐1), the sGC inhibitor 1‐H‐[1,2,4]oxadiazole[4,3‐a]quinoxalin‐1‐one (ODQ) and the non‐selective PDE 5 inhibitor zaprinast) suggested that cGMP enhances the release of NO from autonomic neurons in guinea‐pig colon and in rabbit corpus cavernosum, but the effect of zaprinast was variable.…”

Section: Introductionmentioning

confidence: 99%

Inhibitors of phosphodiesterase 5 (PDE 5) inhibit the nerve‐induced release of nitric oxide from the rabbit corpus cavernosum

Hallén

Wiklund

Gustafsson

2007

British J Pharmacology

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Background and purpose: Nitrergic neurons are important for erectile responses in the corpus cavernosum and impaired signalling results in erectile dysfunction, today treated successfully by oral administration of the selective phosphodiesterase 5 (PDE 5) inhibitors sildenafil, tadalafil and vardenafil. Although the importance of nitrergic neurons in urogenital function has become evident, it has not been investigated if the PDE 5 inhibitors affect the nerve-induced release of nitric oxide (NO). In a previous study we found that the soluble guanylate cyclase (sGC)/cyclic guanosine 3',5'-monophosphate (cGMP) pathway might modulate nerve-induced release of NO in isolated cavernous tissue. Experimental approach: Electrical field stimulation (EFS 5 Hz, 40 V, 0.3 ms pulse duration, 25 pulses at intervals of 2 min) of rabbit isolated cavernous tissue elicited reproducible, nerve-mediated relaxations in the presence of scopolamine (10 À5 M), guanethidine (10 À5 M) and phenylephrine (3 Â 10 À6 M). In superfusion experiments, nerve stimulation (20 Hz, 40 V, 1 ms) of the cavernous tissue evoked release of NO/NO 2 À , measured by chemiluminescence. Key results: Sildenafil, tadalafil and vardenafil decreased the muscular tone and prolonged the relaxations to nerve stimulation. The evoked release of NO decreased to 72711%, 55716% and 61714% of control, respectively after addition of sildenafil, tadalafil or vardenafil (all 10 À4 M, n ¼ 6-8, po0.05). Conclusions and Implications: Selective PDE 5 inhibitors influence the nerve-induced release of NO, probably via cGMPmediated negative feedback. This negative feedback might explain why priapism is not seen during monotherapy with the PDE inhibitors.

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“…The nitrite reduction system alone has previously been used in our laboratory in for example studying NO release from guinea-pig colon [32] and from rabbit corpus cavernosum [33]. Sen et al [34] used a partly similar system (HPLC coupled to nitrite reduction) to analyze inorganic nitrite in food, saliva and urine.…”

Section: Discussionmentioning

confidence: 99%

Formation of new bioactive organic nitrites and their identification with gas chromatography–mass spectrometry and liquid chromatography coupled to nitrite reduction

Nilsson

Lundgren²,

Agvald

et al. 2011

Biochemical Pharmacology

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Nerve-induced release of nitric oxide from the rabbit corpus cavernosum is modulated by cyclic guanosine 3′,5′-monophosphate

Cited by 10 publications

References 36 publications

Protective effect of quercetin, a polyphenolic compound, on mouse corpus cavernosum

Protective effect of quercetin, a polyphenolic compound, on mouse corpus cavernosum

Inhibitors of phosphodiesterase 5 (PDE 5) inhibit the nerve‐induced release of nitric oxide from the rabbit corpus cavernosum

Formation of new bioactive organic nitrites and their identification with gas chromatography–mass spectrometry and liquid chromatography coupled to nitrite reduction

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