Nerve Guidance Conduits from Aligned Nanofibers: Improvement of Nerve Regeneration through Longitudinal Nanogrooves on a Fiber Surface

Huang, Chen; Ouyang, Yuanming; Niu, Haitao; He, Nanfei; Ke, Qinfei; Jin, Xiangyu; Li, Dawei; Fang, Jun; Liu, Wanjun; Fan, Cunyi; Lin, Tong

doi:10.1021/am509227t

Cited by 127 publications

(81 citation statements)

References 47 publications

(85 reference statements)

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“…The electrophysiological analysis was conducted using previous method43. The electrophysiological evaluation was performed at 1 month intervals up to a period of 3 months using an electromyograph machine (Nuocheng, Shanghai, China) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

A 3D-engineered porous conduit for peripheral nerve repair

Tao

Wang

et al. 2017

Sci Rep

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End-to-end neurorrhaphy is the most commonly used method for treating peripheral nerve injury. However, only 50% of patients can regain useful function after treating with neurorrhaphy. Here, we constructed a 3D-engineered porous conduit to promote the function recovery of the transected peripheral nerve after neurorrhaphy. The conduit that consisted of a gelatin cryogel was prepared by molding with 3D-printed moulds. Due to its porous structure and excellent mechanical properties, this conduit could be collapsed by the mechanical force and resumed its original shape after absorption of normal saline. This shape-memory property allowed a simply surgery process for installing the conduits. Moreover, the biodegradable conduit could prevent the infiltration of fibroblasts and reduce the risk of scar tissue, which could provide an advantageous environment for nerve regeneration. The efficiency of the conduits in assisting peripheral nerve regeneration after neurorrhaphy was evaluated in a rat sciatic nerve transected model. Results indicated that conduits significantly benefitted the recovery of the transected peripheral nerve after end-to-end neurorrhaphy on the static sciatic index (SSI), electrophysiological results and the re-innervation of the gastrocnemius muscle. This work demonstrates a biodegradable nerve conduit that has potentially clinical application in promoting the neurorrhaphy.

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Section: Methodsmentioning

confidence: 99%

A 3D-engineered porous conduit for peripheral nerve repair

Tao

Wang

et al. 2017

Sci Rep

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“…119 Overall, longitudinally aligned nanofibers and nanogrooves improve peripheral nerve generation, and have been shown to be beneficial for histological outcomes, nerve conductivity, muscle atrophy, and functional performance. 120 More specifically, axially aligned fibers have been shown to be superior to circumferentially or randomly aligned fibers in NGC repair of 15 mm nerve defects. 121 Furthermore, cell orientation, migration, and differentiation have been shown to be markedly improved when using highly aligned nanofiber scaffolds.…”

Section: Fabricationmentioning

confidence: 99%

Biomimetic neural scaffolds: a crucial step towards optimal peripheral nerve regeneration

et al. 2018

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Peripheral nerve injury is a common disease that affects more than 20 million people in the United States alone and remains a major burden to society. The current gold standard treatment for critical-sized nerve defects is autologous nerve graft transplantation; however, this method is limited in many ways and does not always lead to satisfactory outcomes. The limitations of autografts have prompted investigations into artificial neural scaffolds as replacements, and some neural scaffold devices have progressed to widespread clinical use; scaffold technology overall has yet to be shown to be consistently on a par with or superior to autografts. Recent advances in biomimetic scaffold technologies have opened up many new and exciting opportunities, and novel improvements in material, fabrication technique, scaffold architecture, and lumen surface modifications that better reflect biological anatomy and physiology have independently been shown to benefit overall nerve regeneration. Furthermore, biomimetic features of neural scaffolds have also been shown to work synergistically with other nerve regeneration therapy strategies such as growth factor supplementation, stem cell transplantation, and cell surface glycoengineering. This review summarizes the current state of neural scaffolds, highlights major advances in biomimetic technologies, and discusses future opportunities in the field of peripheral nerve regeneration.

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“…15 The aligned fibers can provide a topographical cue to direct and enhance axonal extension during regeneration. 16–18 Additionally, bioactive molecules such as growth factors can also be readily incorporated in the conduit. Sustained release of these effectors from the conduit has resulted in a better outcome for peripheral nerve regeneration.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers

Xue

Yang

O’Connor

et al. 2017

ACS Appl. Mater. Interfaces

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Seeding nerve guidance conduits with Schwann cells can improve the outcome of peripheral nerve injury repair. Bone marrow stem cells (BMSCs) represent a good choice of cell source as they can differentiate into Schwann cells under appropriate conditions. In this work, we systematically investigated the differentiation of BMSCs into Schwann cells on scaffolds comprising electrospun fibers. We changed the alignment, diameter, and surface properties of the fibers to optimize the differentiation efficiency. The uniaxial alignment of fibers not only promoted the differentiation of BMSCs into Schwann cells but also dictated the morphology and alignment of the derived cells. Coating the surface of aligned fibers with laminin further enhanced the differentiation and thus increased the secretion of neurotrophins. When co-cultured with PC12 cells or chick dorsal root ganglion, the as-derived Schwann cells were able to promote the outgrowth of neurites from cell bodies and direct their extension along the fibers, demonstrating the positive impacts of both the neurotrophic effect and the morphological contact guidance. This work offers a promising strategy for integrating fiber guidance with stem cell therapy to augment peripheral nerve injury repair.

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Nerve Guidance Conduits from Aligned Nanofibers: Improvement of Nerve Regeneration through Longitudinal Nanogrooves on a Fiber Surface

Cited by 127 publications

References 47 publications

A 3D-engineered porous conduit for peripheral nerve repair

A 3D-engineered porous conduit for peripheral nerve repair

Biomimetic neural scaffolds: a crucial step towards optimal peripheral nerve regeneration

Differentiation of Bone Marrow Stem Cells into Schwann Cells for the Promotion of Neurite Outgrowth on Electrospun Fibers

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