Nerve guidance conduit promoted peripheral nerve regeneration in rats

Long, Qingshan; Wu, Bingshan; Yu, Yang; Wang, Shanhong; Shen, Yiwen; Bao, Qinghua; Xu, Feng

doi:10.1111/aor.13881

Cited by 22 publications

(20 citation statements)

References 37 publications

(64 reference statements)

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“…Autologous nerve transplantation has many problems, including limited sources, functional destruction of nerve donor sites, and many clinical complications, whereas allogeneic nerve transplantation has inevitable immune rejection problems [4]. In recent years, tissue engineering nerve conduits with properties beyond autologous nerve grafts have become a potential alternative for repair after PNI [5,6].…”

Section: Introductionmentioning

confidence: 99%

Establishment of FK506‐Enriched PLGA Nanomaterial Neural Conduit Produced by Electrospinning for the Repair of Long‐Distance Peripheral Nerve Injury

Chu

et al. 2022

Journal of Nanomaterials

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Peripheral nerve injury (PNI) is a serious complication of trauma. Autologous nerve transplantation is the gold standard for the treatment of long-distance peripheral nerve defects, but is often limited by insufficient donor sites, postoperative pain, and paresthesia at the donor site. Peripheral nerve tissue engineering has led to the development of neural conduits to replace autologous nerve grafts. This study aimed to evaluate a new type of electrospun nanomaterial neural conduit, enriched with tacrolimus (FK506), which is an FDA-approved immunosuppressant, for the repair of long-distance peripheral nerve injuries. Poly (lactic-co-glycolic acid) (PLGA) nanofibrous films, with FK506, were prepared by electrostatic spinning and rolled into hollow cylindrical nerve vessels with an inner diameter of 1 mm and length of 15 mm. Material characterization, mechanical testing, degradation, drug release, cytotoxicity, cell proliferation, and migration assays were performed in vitro. Long-distance sciatic nerve injuries in rats were repaired in vivo using electrospun nerve conduit bridging, and nerve regeneration and muscle and motor function recovery were evaluated by gait analysis, electrophysiology, and neuromuscular histology. Compared to PLGA, the PLGA/FK506 nanomaterial neural conduit showed little change in morphology, mechanical properties, and chemical structure. In vitro, PLGA/FK506 showed lower cytotoxicity and better biocompatibility and effectively promoted the proliferation, adhesion, and migration of Schwann cells. In vivo, PLGA/FK506 had a better effect on sciatic nerve index, compound muscle action potential intensity and delay time, and nerve regeneration quality 12 weeks post-transplantation, effectively promoting long-distance defect sciatic nerve regeneration and functional recovery in rats. FK506-enriched PLGA nanomaterial neural conduits offer an effective method for repairing long-distance peripheral nerve injury and have potential clinical applications.

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Section: Introductionmentioning

confidence: 99%

Establishment of FK506‐Enriched PLGA Nanomaterial Neural Conduit Produced by Electrospinning for the Repair of Long‐Distance Peripheral Nerve Injury

Chu

et al. 2022

Journal of Nanomaterials

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“…The diameters of the regenerated nerves in the CSNPs/OBC, NGF@CSNPs/OBC, and autograft groups were 0.50, 0.58, and 0.63 mm at the fourth week (Figure B) and 0.83, 1.00, and 1.28 mm at the ninth week (Figure C), respectively. As time progressed, the regenerated nerves became thicker, and the repair effect of the NGF@CSNPs/OBC conduit was significantly ( p < 0.01) superior to the CSNPs/OBC conduit, indicating that NGF could promote nerve regeneration . However, the repair effects of both the groups appeared slightly weaker than the autograft group, which might be attributed to the limited repair time for a 10 mm gap.…”

Section: Resultsmentioning

confidence: 99%

“…As time progressed, the regenerated nerves became thicker, and the repair effect of the NGF@CSNPs/OBC conduit was significantly (p < 0.01) superior to the CSNPs/OBC conduit, indicating that NGF could promote nerve regeneration. 48 However, the repair effects of both the groups appeared slightly weaker than the autograft group, which might be attributed to the limited repair time for a 10 mm gap.…”

Section: Growth and Attachment Of Scs In Nerve Guidancementioning

confidence: 94%

In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration

et al. 2021

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Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries. Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts. In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF. A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed. In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells. When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks. At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve. In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.

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“…However, surgical complications include nerve defects at the donor site, limited donor sources, and easy induction of infection. Therefore, fabricating various artificial nerve conduits based on tissue engineering as a substitute for autografts is currently an area of focus in nerve regeneration research ( Long et al, 2021 ). The common nerve conduits in recent studies consist of autogenous conduits and artificial nerve conduits.…”

Section: Nerve Conduitsmentioning

confidence: 99%

“…Meanwhile, there is the risk of long-term immunosuppression after transplantation and infection, especially under circumstances where the structures of cranial and facial tissues are complex and the bacterial flora is diverse ( Husain et al, 2003 ; Singh et al, 2005 ; Cuellar-Rodriguez et al, 2009 ). In recent years, the advantages of nerve conduits combined with tissue engineering have been realized in the field of nerve transplantation ( Zor et al, 2010 ; Szynkaruk et al, 2013 ; Roche et al, 2015a ; Long et al, 2021 ). Researchers are incorporating absorbable and non-absorbable materials, such as silica gel, polyhydroxy acid, poly (lactate-glycolic acid) graphene foam, and polycaprolactone, in these new conduits ( Han et al, 2019 ; Bahremandi Tolou et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Donors for nerve transplantation in craniofacial soft tissue injuries

Sun

Zhong

et al. 2022

Front. Bioeng. Biotechnol.

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Neural tissue is an important soft tissue; for instance, craniofacial nerves govern several aspects of human behavior, including the expression of speech, emotion transmission, sensation, and motor function. Therefore, nerve repair to promote functional recovery after craniofacial soft tissue injuries is indispensable. However, the repair and regeneration of craniofacial nerves are challenging due to their intricate anatomical and physiological characteristics. Currently, nerve transplantation is an irreplaceable treatment for segmental nerve defects. With the development of emerging technologies, transplantation donors have become more diverse. The present article reviews the traditional and emerging alternative materials aimed at advancing cutting-edge research on craniofacial nerve repair and facilitating the transition from the laboratory to the clinic. It also provides a reference for donor selection for nerve repair after clinical craniofacial soft tissue injuries. We found that autografts are still widely accepted as the first options for segmental nerve defects. However, allogeneic composite functional units have a strong advantage for nerve transplantation for nerve defects accompanied by several tissue damages or loss. As an alternative to autografts, decellularized tissue has attracted increasing attention because of its low immunogenicity. Nerve conduits have been developed from traditional autologous tissue to composite conduits based on various synthetic materials, with developments in tissue engineering technology. Nerve conduits have great potential to replace traditional donors because their structures are more consistent with the physiological microenvironment and show self-regulation performance with improvements in 3D technology. New materials, such as hydrogels and nanomaterials, have attracted increasing attention in the biomedical field. Their biocompatibility and stimuli-responsiveness have been gradually explored by researchers in the regeneration and regulation of neural networks.

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Nerve guidance conduit promoted peripheral nerve regeneration in rats

Cited by 22 publications

References 37 publications

Establishment of FK506‐Enriched PLGA Nanomaterial Neural Conduit Produced by Electrospinning for the Repair of Long‐Distance Peripheral Nerve Injury

Establishment of FK506‐Enriched PLGA Nanomaterial Neural Conduit Produced by Electrospinning for the Repair of Long‐Distance Peripheral Nerve Injury

In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration

Donors for nerve transplantation in craniofacial soft tissue injuries

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