Nerve-growth-factor-treated and v-<i>src</i>-expressing PC 12 cells: a model for neuronal differentiation

Hecker, G; Lewis, Deborah L.; Rausch, Dianne M.; Jelsema, Carole L.

doi:10.1042/bst0190385

Cited by 7 publications

(4 citation statements)

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“…For example, infection of avian myoblasts, retinoblasts, or chondroblasts with RSV maintains these cells in a proliferative state and blocks differentiation into myotubes, neuroretinal cells, epidermal cells, or chondrocytes, respectively (Muto et al 1977, Yoshimura et al 1981, Crisanti-Combes et al 1982, Alema & Tato 1987. In contrast, introduction of v-Src into PC12 cells or immature sympathetic neurons induces neurite outgrowth and terminal differentiation into neuron-like cells (Alema et al 1985, Haltmeier & Rohrer 1990, Hecker et al 1991. As discussed previously, these dramatic alterations in cell differentiation induced by v-Src do not imply that cellular Src mediates these effects when activated under natural conditions.…”

Section: Differentiationmentioning

confidence: 99%

Cellular Functions Regulated by SRC Family Kinases

Thomas

Brugge

1997

Annu. Rev. Cell Dev. Biol.

2,298

2,140

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Src family protein tyrosine kinases are activated following engagement of many different classes of cellular receptors and participate in signaling pathways that control a diverse spectrum of receptor-induced biological activities. While several of these kinases have evolved to play distinct roles in specific receptor pathways, there is considerable redundancy in the functions of these kinases, both with respect to the receptor pathways that activate these kinases and the downstream effectors that mediate their biological activities. This chapter reviews the evidence implicating Src family kinases in specific receptor pathways and describes the mechanisms leading to their activation, the targets that interact with these kinases, and the biological events that they regulate.

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Section: Differentiationmentioning

confidence: 99%

Cellular Functions Regulated by SRC Family Kinases

Thomas

Brugge

1997

Annu. Rev. Cell Dev. Biol.

2,298

2,140

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“…These protein kinases can be activated by a variety of extracellular signals, and interact with multiple intracellular substrates, including focal adhesion kinase (FAK) 1 , p130Cas, structural proteins such as cortactin, and connexin 43, and stimulate other signaling pathways within the cell. Src family kinases have been linked to cellular events such as cell adhesion, cytoskeletal rearrangements, cell migration, regulation of the cell cycle, apoptosis, and differentiation ( Boschek et al, 1981 ; Hecker et al, 1991 ; Twamley-Stein et al, 1993 ; Kaplan et al, 1995 ; Rodier et al, 1995 ; Huang et al, 1997 ).…”

mentioning

confidence: 99%

Morphological Differentiation of Oligodendrocytes Requires Activation of Fyn Tyrosine Kinase

Osterhout

Wolven

Wolf

et al. 1999

The Journal of Cell Biology

211

215

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In the central nervous system, myelination of axons occurs when oligodendrocyte progenitors undergo terminal differentiation and initiate process formation and axonal ensheathment. Although it is hypothesized that neuron-oligodendrocyte contact initiates this process, the molecular signals are not known. Here we find that Fyn tyrosine kinase activity is upregulated very early during oligodendrocyte progenitor cell differentiation. Concomitant with this increase is the appearance of several tyrosine phosphorylated proteins present only in differentiated cells. The increased tyrosine kinase activity is specific to Fyn, as other Src family members are not active in oligodendrocytes. To investigate the function of Fyn activation on differentiation, we used Src family tyrosine kinase inhibitors, PP1 and PP2, in cultures of differentiating oligodendrocyte progenitors. Treatment of progenitors with these compounds prevented activation of Fyn and reduced process extension and myelin membrane formation. This inhibition was reversible and not observed with related inactive analogues. A similar effect was observed when a dominant negative Fyn was introduced in progenitor cells. These findings strongly suggest that activation of Fyn is an essential signaling component for the morphological differentiation of oligodendrocytes.

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“…A possible role of Src in myoblasts, retinoblasts and chondrocytes differentiation was demonstrated using v‐Src (Muto et al 1977; Yoshimura et al 1981; Crisanti‐Combes et al 1982; Alema & Tato, 1987). Fyn and Lyn were implicated in osteoclast differentiation (Kaabeche et al 2004), while v‐Src promotes PC12 differentiation into neurone‐like cells (Alema et al 1985; Haltmeier & Rohrer, 1990; Hecker et al 1991). Therefore, we evaluated the expression of Src in trophoblasts and its activation after FBS addition, a factor known to induce trophoblast differentiation (Daoud et al 2005 a ).…”

Section: Discussionmentioning

confidence: 99%

“…Kaabeche et al (2004) showed that degradation of Fyn and Lyn, induced by constitutive fibroblast growth factor receptor‐2 activation, supported osteoblast differentiation. In contrast, introduction of v‐Src into PC12 cells or immature neurones induced neurite outgrowth and terminal differentiation into neurone‐like cells (Alema et al 1985; Haltmeier & Rohrer, 1990; Hecker et al 1991). Furthermore, c‐Src was implicated in human trophoblast differentiation (Rebut‐Bonneton et al 1993), while Src, Yes and Lyn were activated during rat trophoblast giant cell differentiation (Kamei et al 1997).…”

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confidence: 94%

Src family kinases play multiple roles in differentiation of trophoblasts from human term placenta

Daoud

Rassart

Masse

et al. 2006

The Journal of Physiology

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Tyrosine phosphorylation plays a major role in controlling many biological processes in different cell types. Src family kinases (SFKs) are one of the most studied groups of tyrosine kinases and can mediate a variety of signalling pathways. However, little is known about the expression of SFKs in human term placenta and their implication in trophoblast differentiation. Therefore, we examined the expression profile of SFK members over time in culture and their implication in differentiation. In vitro, freshly isolated cytotrophoblast cells, cultured in 10% fetal bovine serum (FBS), spontaneously aggregate and fuse to form multinucleated cells that resemble phenotypically mature syncytiotrophoblasts, that concomitantly produce human chorionic gonadotropin (hCG) and human placental lactogen (hPL). In this study, we showed that trophoblasts expressed all SFK members and some of them are expressed as different splice variants. Moreover, using real-time PCR, this study showed two different expression profiles of SFKs in human trophoblasts during culture. In addition, the protein level and phosphorylation status of Src were evaluated using specific antibodies. Src was rapidly phosphorylated at Tyr-416 and dephosphorylated at Tyr-527 after FBS addition. Surprisingly, inhibition of SFKs by 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo[3,4-d] pyrimidine (PP2) or herbimycin A had different effects on trophoblast differentiation. While herbimycin A inhibited morphological and hormonal differentiation, PP2 stimulated hormonal differentiation and inhibited cell adhesion and spreading with no effect on cell fusion. In summary, this study showed that SFKs play different roles in trophoblast differentiation, probably depending on SFK members activated. Thus, this study increases our knowledge and understanding of pathology related to impaired trophoblast differentiation such as pre-eclampsia and trophoblast neoplasm.

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Nerve-growth-factor-treated and v-src-expressing PC 12 cells: a model for neuronal differentiation

Cited by 7 publications

References 0 publications

Cellular Functions Regulated by SRC Family Kinases

Cellular Functions Regulated by SRC Family Kinases

Morphological Differentiation of Oligodendrocytes Requires Activation of Fyn Tyrosine Kinase

Src family kinases play multiple roles in differentiation of trophoblasts from human term placenta

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