Nerve growth factor in medium conditioned by embryonic chicken heart cells

Norrgren, Gunilla; Ebendal, Ted

doi:10.1016/0736-5748(86)90015-8

Cited by 16 publications

(2 citation statements)

References 40 publications

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“…It is obvious that antibodies to mouse NGF at higher concentrations than needed to block mouse NGF do in fact in many cases inhibit the biological activity of NGF from a number of other species, including human and chicken NGF (Norrgren et al, 1980;Norrgren and Ebendal, 1986;Belew and Ebendal, 1986;Hallbook et al, 1988). However, there is variation between different anti-mouse NGF antibodies in the ability to block heterologous NGFs (Dimberg and , and in some instances the blocking ability is nearly as good as the ability to block mouse NGF.…”

Section: Discussionmentioning

confidence: 99%

Recombinant human β‐nerve growth factor (NGF): Biological activity and properties in an enzyme immunoassay

Söderström

Hallböök

Ibáñez

et al. 1990

J of Neuroscience Research

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Nerve growth factor (NGF) supports sympathetic and sensory neurons in the peripheral nervous system and also functions in the development and maintenance of cholinergic neurons in the basal forebrain. NGF distribution can be studied in the brain of the rat and mouse with the use of a sensitive two-site enzyme immunoassay (EIA) for mouse NGF. It would be of interest to measure the NGF protein also in the human brain, especially against the background that the cholinergic neurons are severely deteriorated in senile dementia of the Alzheimer type. The limited immunological cross-reactivity between NGFs from different species has previously hampered attempts to determine levels of the human NGF. We have now examined the biological activity and immunological properties of human recombinant NGF protein in medium conditioned by COS cells transfected with the human NGF gene. The human NGF behaved similar to mouse NGF in a sympathetic ganglion bioassay. The monoclonal antibody 27/21 to mouse NGF was shown to effectively block the activity of both the human recombinant NGF and mouse native NGF. A two-site EIA using monoclonal antibody 27/21 was optimized. Under the conditions used, the EIA detected the human recombinant NGF with the same sensitivity (1 pg/ml) as shown for the mouse NGF. It should now be possible to test this EIA also on homogenized tissue to examine human NGF in brain samples from Alzheimer patients and age-matched controls.

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Section: Discussionmentioning

confidence: 99%

Recombinant human β‐nerve growth factor (NGF): Biological activity and properties in an enzyme immunoassay

Söderström

Hallböök

Ibáñez

et al. 1990

J of Neuroscience Research

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“…against mouse NGF can cross-react with a chicken form of NGF Young et al, 1975;Ebendal et al, 1982;Norrgren and Ebendal, 1986;Abrahamson et al, 1986Abrahamson et al, , 1987b. Furthermore, these studies demonstrate that chicken NGF shares with mouse NGF not only common antigenic determinants but also similar physicochemical and biological properties.…”

Section: Effect Of Culture Conditionsmentioning

confidence: 92%

Chicken NGF and Non‐NGF trophic factor synthesis and release by sciatic nerves in vitro

Ferguson

Williams

Rush

1989

J of Neuroscience Research

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The time course of production and release of nerve growth factor (NGF) and non-NGF neuronotrophic factors for sympathetic neurons by chicken and rat sciatic nerves in culture was examined. These tissues actively synthesize and release neuronotrophic activity as metabolically poisoning nerves with azide dramatically reduced the amount of trophic activity released into the culture medium. The sustained release of this activity also was shown to be dependent on the presence of low-molecular-weight dialysable molecules present in foetal calf serum and amniotic fluid from day 11 chicken embryos. Affinity-purified antimouse NGF antibodies were used to show that sciatic nerves in culture release both NGF and non-NGF trophic factors. These antibodies inhibited all bioactivity of both mouse NGF and of a partially purified preparation of chicken NGF. Immunoblot studies confirm that the antibodies recognize both rodent and avian NGF. Excess antibody inhibited only about 50% of the trophic activity in media conditioned over rat or chicken nerves for the first 24 hr. Relatively similar amounts of this non-NGF trophic activity were released throughout 6 days in culture, and this trophic activity kept sympathetic neurons alive in culture in the absence of NGF for more than 4 days. NGF levels were quantified with a two-site enzyme-linked immunoassay and found to parallel changes in NGF bioactivity. Rat nerves released increasing amounts of NGF with time in culture. Whole chicken sciatic nerves, however, released decreasing amounts of NGF with time in culture, but when these nerves were desheathed by removal of the epineurium and attached tissue, the pattern of NGF release was similar to that observed in the smaller rat sciatic nerves. These studies therefore characterize antibodies recognizing chicken NGF, demonstrate that peripheral nerve tissue synthesize trophic factors other than NGF, and identify factors that influence NGF synthesis.

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Structure and expression of the chicken beta nerve growth factor gene.

Ebendal¹,

Larhammar²,

Persson³

1986

The EMBO Journal

120

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The 3′ exon of the chicken beta nerve growth factor (NGF) gene was isolated by the use of a murine cDNA probe. DNA sequence analysis of the clone suggests a mature chicken NGF protein of 118 amino acids, showing approximately 85% homology to mouse and human NGF. In addition to this conservation of the mature NGF, parts of the propeptide and the untranslated 3′ end of the NGF gene are also highly homologous in chicken, human and mouse. Therefore, these sequences probably subserve important functions. Expression of NGF mRNA in various chicken tissues was examined by RNA blot analysis with a chicken NGF probe. A single mRNA of 1.3 kb was detected at high levels in heart and brain of 10‐week‐old roosters, and, at lower levels in spleen, liver and skeletal muscle. These data suggest a correlation between NGF expression and the density of sympathetic innervation in peripheral organs, in analogy with findings for mammalian tissues. In the adult avian brain, NGF mRNA is found at higher concentration in the optic tectum and cerebellum than in the cortex and hippocampus. This pattern of NGF expression differs from that previously described for the rat brain. During late stages of development (day 18), NGF mRNA was expressed both in heart and brain of embryos but at lower levels than in the adult.

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Nerve growth factor in medium conditioned by embryonic chicken heart cells

Cited by 16 publications

References 40 publications

Recombinant human β‐nerve growth factor (NGF): Biological activity and properties in an enzyme immunoassay

Recombinant human β‐nerve growth factor (NGF): Biological activity and properties in an enzyme immunoassay

Chicken NGF and Non‐NGF trophic factor synthesis and release by sciatic nerves in vitro

Structure and expression of the chicken beta nerve growth factor gene.

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