Nerve Growth Factor-Beta, purified from bull seminal plasma, enhances corpus luteum formation and conceptus development in Bos taurus cows

Stewart, Jamie L.; Mercadante, Vitor R G; Dias, Nicholas W; Canisso, Igor F.; Yau, Peter M.; Imai, Brian S.; Lima, F.S.

doi:10.1016/j.theriogenology.2017.10.007

Cited by 26 publications

(33 citation statements)

References 42 publications

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“…Perhaps, these changes affect the sites of action of SP on the sperm plasma membrane and this might partly explain the lack of oscillatory motility we observed. One of the proteins that may possibly be related to sperm motility is the β‐NGF present in SP of several species (Casares‐Crespo, Fernández‐Serrano, Vicente, Marco‐Jiménez, & Viudes‐de‐Castro, 2018; Heinrich & Meyer, 1988; Stewart et al, 2018) and detected in a much higher concentration in camelids (Druart et al, 2013). Castellini et al (2019) determined that rabbit spermatozoon possesses two receptors for the β‐NGF protein (TrKA and p75NTR), and that both are related to sperm motility.…”

Section: Discussionmentioning

confidence: 99%

Incubation of frozen‐thawed llama sperm with seminal plasma

et al. 2020

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Seminal plasma is intimately connected to sperm physiology and particularly in South American Camelids, has demonstrated to be involved in multiple physiological reproductive events. Different percentages of seminal plasma (0%, 10% and 50%) were added to thawed llama semen samples with the objective of evaluating the interaction with cryopreserved sperm over time (0, 1.5 and 3 hr at 37°C). A total of 20 ejaculates from five adult llama males (n = 5; r = 4) were evaluated. A significant decrease in sperm motility, membrane function and live sperm was observed in all thawed samples (0%, 10% and 50%) at 0 hr when compared to raw semen. Neither morphology nor chromatin condensation was altered in all thawed samples (p > .05), but a significant increase in the percentage of spermatozoa with fragmented DNA was observed after thawing all samples versus raw semen. When evaluating thawed samples over time, a significant decrease of motility and membrane function was observed, while the percentages of total live sperm were preserved over the 3 hr of incubation in all final concentrations evaluated. To conclude, the addition of 10% or 50% of seminal plasma was incapable of preserving motility or membrane function of frozen‐thawed llama sperm during 3 hr of incubation.

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Section: Discussionmentioning

confidence: 99%

Incubation of frozen‐thawed llama sperm with seminal plasma

et al. 2020

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“…The dissected 24 follicle wall pieces containing theca and granulosa cells were randomly allocated to receive a culture medium that was either supplemented with puri ed bovine NGF (100 ng/mL, n = 12) or left untreated (control, n = 12). The single plate was incubated at 37 °C in a humidi ed incubator gassed with 5% CO 2 :95% air for 72 h. The NGF used was puri ed from bovine seminal plasma, as described previously (13). The follicle wall pieces were cultured in 0.…”

Section: Isolation Of Follicular Wall Cells and Treatment Allocationmentioning

confidence: 99%

“…Our understanding of the role of NGF in promoting mammalian ovulation became even more complex when it was identi ed as the ovulation-inducing factor in camelid seminal plasma (10,11). Though the ability of the ovary to produce NGF in spontaneously ovulating species is well documented, few studies have evaluated whether the introduction of NGF from seminal plasma at time of breeding may have a role within the bovine hypothalamic-pituitary-ovarian axis (12)(13)(14)(15). In camelids, intrauterine absorption of seminal plasma NGF into systemic circulation occurs within 15 min of copulation, after which it stimulates the preovulatory LH peak from the anterior pituitary gland and exerts a dose-dependent luteotropic effect on the developing corpus luteum (CL) (16).…”

Section: Introductionmentioning

confidence: 99%

“…In camelids, intrauterine absorption of seminal plasma NGF into systemic circulation occurs within 15 min of copulation, after which it stimulates the preovulatory LH peak from the anterior pituitary gland and exerts a dose-dependent luteotropic effect on the developing corpus luteum (CL) (16). Studies have shown that NGF retains its luteotropic properties when administered systemically to cattle (12)(13)(14). While the luteotropic effect in camelids is attributed to the prolonged LH secretion from the pituitary, in cattle, there is evidence that NGF from the seminal plasma may act directly on the ovary (5).…”

Section: Introductionmentioning

confidence: 99%

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Direct Effects of Nerve Growth Factor-β, Purified From Bull Seminal Plasma,on Steroidogenesis and Angiogenic Markers of Thebovine Pre-ovulatory Follicle

Stewart

Gao

Flaws

et al. 2020

Preprint

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Nerve growth factor-β (NGF) is critical for ovulation in the mammalian ovary and is luteotrophic when administered systemically to camelids and cattle. This study aimed to assess the direct effects of purified bovine NGF on steroidogenesis and angiogenic markers in the bovine pre-ovulatory follicle. A chort of Holstein heifers were synchronized with a standard protocol and heifers with the preovulatory follicle (≥ 12 mm) had an the ovary containing the dominant follicle removed via colpotomy. Pre-ovulatory follicles were dissected in 24 pieces containing theca and granulosa cells that were randomly allocated to receive either cultured in media supplemented with purified bovine NGF (100 ng/mL) or untreated (control) for 72 h. The supernatant media was harvested for determination of progesterone, testosterone, and estradiol, whereas explants were used for mRNA analyses for steroidogenesis and angiogenic markers. Treatment of follicle tissue with NGF upregulated gene expression of steroidogenic enzyme HDS17B ( P = 0.04) and increased testosterone production ( P < 0.01). However, NGF treatment did not alter production of progesterone ( P = 0.81) or estradiol ( P = 0.14). Consistently, gene expression of steroidogenic enzymes responsible for producing these hormones ( STAR , CYP11A1 , HSD3B , CYP17A1 , CYP19A1 ) were unaffected by NGF treatment ( P ≥ 0.31). Treatment with NGF downregulated gene expression of the angiogenic enzyme FGF2 ( P = 0.02) but did not alter PGES ( P = 0.63), VEGFA ( P = 0.44), and ESR1 ( P = 0.77). Collectively, these results demonstrate that NGF from seminal plasma may interact directly with the bovine pre-ovulatory follicle to alter downstream steroidogenesis and luteal development.

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“…Treated females displayed a greater plasma progesterone concentration, improving conceptus development and ultimately conception rate. In addition, concentration of NGF in bovine seminal plasma has been correlated with fertility in dairy and beef bulls (Stewart et al 2018b).…”

Section: Potential Mechanism Of Seminal Plasma β-Ngf In Spontaneous Omentioning

confidence: 99%

New insights of the role of β-NGF in the ovulation mechanism of induced ovulating species

et al. 2019

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The type of stimuli triggering GnRH secretion has been used to classify mammalian species into two categories: spontaneous or induced ovulators. In the former, ovarian steroids produced by a mature follicle elicit the release of GnRH from the hypothalamus, but in the latter, GnRH secretion requires coital stimulation. However, the mechanism responsible for eliciting the preovulatory LH surge in induced ovulators is still not well understood and seems to vary among species. The main goal of this review is to offer new information regarding the mechanism that regulates coitus-induced ovulation. Analysis of several studies documenting the discovery of β-NGF in seminal plasma and its role in the control of ovulation in the llama and rabbit will be described. We also propose a working hypothesis regarding the sites of action of β-NGF in the llama hypothalamus. Finally, we described the presence of β-NGF in the semen of species categorized as spontaneous ovulators, mainly cattle, and its potential role in ovarian function. The discovery of this seminal molecule and its ovulatory effect in induced ovulators challenges previous concepts about the neuroendocrinology of reflex ovulation and has provided a new opportunity to examine the mechanism(s) involved in the cascade of events leading to ovulation. The presence of the factor in the semen of induced as well as spontaneous ovulators highlights the importance of understanding its signaling pathways and mechanism of action and may have broad implications in mammalian fertility.

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Nerve Growth Factor-Beta, purified from bull seminal plasma, enhances corpus luteum formation and conceptus development in Bos taurus cows

Cited by 26 publications

References 42 publications

Incubation of frozen‐thawed llama sperm with seminal plasma

Incubation of frozen‐thawed llama sperm with seminal plasma

Direct Effects of Nerve Growth Factor-β, Purified From Bull Seminal Plasma,on Steroidogenesis and Angiogenic Markers of Thebovine Pre-ovulatory Follicle

New insights of the role of β-NGF in the ovulation mechanism of induced ovulating species

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