Nerve growth factor and burn wound healing: Update of molecular interactions with skin cells

Baassiri, Mahmoud El; Dosh, Laura; Haidar, Hanine; Gerges, Alice; Baassiri, Silma; Leone, Angelo; Rappa, Francesca

doi:10.1016/j.burns.2022.11.001

Cited by 25 publications

(17 citation statements)

References 100 publications

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“…Promoted necroptosis will inevitably induce cell rupture and extensive content leakage, which will also finally lead to excess ROS and serious inflammation 40 . Obviously, ROS accumulation and inflammation stimulation are regarded as critical factors in skin fibroblasts proliferation 16,41 . This is possibly how CSE deficiency accelerated skin fibroblasts proliferation via promoted necroptosis.…”

Section: Discussionmentioning

confidence: 99%

“…40 Obviously, ROS accumulation and inflammation stimulation are regarded as critical factors in skin fibroblasts proliferation. 16,41 This is possibly how CSE deficiency accelerated skin fibroblasts proliferation via promoted necroptosis. Previous evidence has demonstrated that necroptosis was participated in cardiovascular, neurological, digestive and other systemic diseases.…”

Section: Discussionmentioning

confidence: 99%

“…The exact pathophysiological mechanism of excessive proliferation in skin fibroblasts is not completely clear at present, which may be related to inflammation, oxidative stress, growth factors secretion, collagen metabolism, congenital heredity and some other factors. 16 Among them, transforming growth factor-β 1 (TGF-β 1 ) initiates and terminates tissue repair, participates in epithelial regeneration, interstitial hyperplasia, fibroblast fibrosis, collagen production and angiogenesis during wound healing. 17 TGF-β 1 is involved in proliferation, differentiation, migration and apoptosis in skin fibroblasts and the pathological process of skin fibrotic disease can be imitated by TGFβ 1 stimulation.…”

Section: Introductionmentioning

confidence: 99%

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Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis

Li,

Chen,

Liu

et al. 2023

Experimental Dermatology

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An excessive proliferation of skin fibroblasts usually results in different skin fibrotic diseases. Hydrogen sulphide (H2S) is regarded as an important endogenous gasotransmitter with various functions. The study aimed to investigate the roles and mechanisms of H2S on primary mice skin fibroblasts proliferation. Cell proliferation and collagen synthesis were assessed with the expression of α‐smooth muscle actin (α‐SMA), proliferating cell nuclear antigen (PCNA), Collagen I and Collagen III. The degree of oxidative stress was evaluated by dihydroethidium (DHE) and MitoSOX staining. Mitochondrial membrane potential (ΔΨm) was detected by JC‐1 staining. Necroptosis was evaluated with TDT‐mediated dUTP nick end labelling (TUNEL) and expression of receptor‐interacting protein kinase 1 (RIPK1), RIPK3 and mixed lineage kinase domain‐like protein (MLKL). The present study found that α‐SMA, PCNA, Collagen I and Collagen III expression were increased, oxidative stress was promoted, ΔΨm was impaired and positive rate of TUNEL staining, RIPK1 and RIPK3 expression as well as MLKL phosphorylation were all enhanced in skin fibroblasts from cystathionine γ‐lyase (CSE) knockout (KO) mice or transforming growth factor‐β1 (TGF‐β1, 10 ng/mL)‐stimulated mice skin fibroblasts, which was restored by exogenous sodium hydrosulphide (NaHS, 50 μmol/L). In conclusion, endogenous H2S production impairment in CSE‐deficient mice accelerated skin fibroblasts proliferation via promoted necroptosis, which was attenuated by exogenous H2S. Exogenous H2S supplement alleviated proliferation of skin fibroblasts with TGF‐β1 stimulation via necroptosis inhibition. This study provides evidence for H2S as a candidate agent to prevent and treat skin fibrotic diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis

Li,

Chen,

Liu

et al. 2023

Experimental Dermatology

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“…97 Fibroblasts, which are specialised cells responsible for collagen production, move to the wound site and form a new extracellular matrix, providing structural support to the healing tissue. 98 Angiogenesis, or the development of new blood vessels, occurs to restore the vascular network required for nutrition delivery to the healing region. 99 Furthermore, epithelial cells within the wound boundaries multiply and move to cover the wound surface, providing a protective layer.…”

Section: Phase 3: Proliferative Phasementioning

confidence: 99%

Nanotechnology-driven wound healing potential of asiaticoside: a comprehensive review

Kumar,

Mahmood

et al. 2024

RSC Pharm.

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“…NGF promotes broblast and keratinocyte proliferation, extracellular matrix component expression and secretion and it stimulates the proliferation and differentiation of local immune cells, blood vessels and even neurite outgrowth. NGF contributes also to the reestablishment of a normal sensory and sympathetic innervation of the damaged skin [44,45]. NGF has a high concentration in plasma (min 67 pg/mL; max 79 pg/mL; mean 73 ± 4.031 pg/mL) compared to PRP (min 1.19 pg/mL; max 1.30 pg/mL; mean 1.25 ± 0.036 pg/mL) and a low concentration compared to PRP with AMPLEX PLUS technology (min 410 pg/mL; max 431 pg/mL; mean 420 ± 6.403 pg/mL).…”

Section: Nerve Growth Factor (Ngf): Activity and Concentrationsmentioning

confidence: 99%

How to enhance the effects of Platelet Rich Plasma on tissue regeneration: AMPLEX PLUS technology, a new strategy.

Brundo,

Ferruggia,

Contino

et al. 2023

Preprint

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The employment of Platelet-Rich Plasma (PRP) has generated promising results in several fields of aesthetic and regenerative medicine. However, PRP research represents a continuing expanding field, due in part to the lack of standardized protocols and consistent clinical trials. A significant progress has been achieved with the analysis and the elucidation of mechanisms of exosomes. They are extracellular vesicles surrounded by membrane with a crucial role of carriers of different substances involved in tissue repair. These bioactive molecules are growth factors and cytokines capable of stimulating cell proliferation, ensuring tissue regeneration in response to injury. Therefore, scientific research has focused on identifying other matrices rich in growth factors, cytokines, and exosomes to improve the efficacy of PRP. In the present study, we evaluated by enzyme linked immunosorbent assay (ELISA) the concentration trends of 20 growth factors and cytokines in plasma, PRP and PRP with a new technology, a compound derived from colostrum enriched with exosomes. The results showed that the concentration of all analyzed compounds increased significantly in PRP samples with the new technology compared with samples containing only plasma or PRP, suggesting how this new strategy can improve PRP performance and make potential progress in regenerative medicine.

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Nerve growth factor and burn wound healing: Update of molecular interactions with skin cells

Cited by 25 publications

References 100 publications

Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis

Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis

Nanotechnology-driven wound healing potential of asiaticoside: a comprehensive review

How to enhance the effects of Platelet Rich Plasma on tissue regeneration: AMPLEX PLUS technology, a new strategy.

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