Nerve growth factor and basic fibroblast growth factor promote reinnervation by nerve−muscle−endplate grafting

Abstract

Focal administration of NGF and FGF-2 promotes efficacy of the NMEG technique. Muscle Nerve 57: 449-459, 2018.

Immunohistochemical Detection of Motor Endplates in the Long-Term Denervated Muscle

“…Indeed, the outcomes of NMEG-NMZ technique can be further improved by a combination of NMEG-NMZ surgery with other supplementary therapies to obtain a synergistic effect. 27,73…”

“…Triple fluorescence labeling was performed on some sagittal sections from the NMZ (middle segment) of the denervated and contralateral SM muscles in each rat to detect MEPs and intramuscular nerve axons, as described previously, 12,26,27 with some modifications. Briefly, the sections were fixed in Zamboni fixative with 5% sucrose for 20 minutes at 4°C.…”

Sensory Innervation of the Human Soft Palate

“…Quantitative analysis was performed on the stained coronal sections. The intramucosal axonal density was assessed by computing the number of the NF‐immunoreactive (NF‐ir) nerve fibers and the area fraction of the fibers within a section area (1.0 mm 2 ) using ImageJ software v1.45 (National Institutes of Health, Bethesda, MD) as described in our publications (Mu et al, ; Sobotka et al, ). For a given sample, three stained sections at different spatial levels were randomly selected to quantify NF‐ir nerve fibers and areas.…”

“…Distribution of intramucosal sensory nerve axons was assessed using an immunohistochemical method. Specifically, some coronal and sagittal sections were immunostained with monoclonal antibody SMI‐31 as a marker for all axons (Covance Research Products, Berkeley, CA) as described in our previous publications (Mu et al, ; Sobotka et al, ). Briefly, the sections were treated in phosphate‐buffered saline (PBS) containing 0.3% Triton and 2% bovine serum albumin (BSA) for 30 minutes, incubated with SMI‐31 primary antibody (1:800) in PBS containing 0.03% Triton at 4°C overnight, incubated with anti‐mouse biotinylated secondary antibody (1:1,000; Vector Labs, Burlingame, CA) for 2 hours, processed with avidin–biotin complex method using a VectaStain ABC kit (1:1,000; ABC Elite; Vector), and treated with diaminobenzidine–nickel as chromogen to visualize peroxidase labeling.…”

Polymer Scaffolds for Biomedical Applications in Peripheral Nerve Reconstruction

“…Compared with autogenous nerve transplantation, bridging a 30-mm nerve defect using collagen filaments achieved a good result [ 18 ]. Although the collagen nerve guide channel has been successful in nerve repair, collagen is relatively expensive, and because of the lack of mechanical strength, challenging to deal with in the suturing process [ 19 ]. In terms of combining cytokines, a comparative experiment of artificial nerve catheters has been carried out, including collagen extract alone and autologous nerve transplantation.…”