Nerve compression induces activating transcription factor 3 in neurons and Schwann cells in diabetic rats

Dahlin, Lars B.; Stenberg, Lena; Luthman, Holger; Thomsen, Niels

doi:10.1097/wnr.0b013e328302f4ec

Cited by 22 publications

(19 citation statements)

References 23 publications

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“…However, our approach was to relate axonal outgrowth with possible activation and apoptotic events concerning the Schwann cells in relation to certain blood glucose levels in male and female rats, without interfering with the processes in the treatment of insulin, which would be required in long-term studies in BB rats. Finally, the nature of the nerve injury is also relevant in this context, since a nerve compression lesion in diabetic rats induces a different response in the nerve trunk with more ATF-3 stained Schwann cells in diabetic BB rats than in diabetic GK and healthy rats [49]. Taken together, in future studies of nerve regeneration and neuropathy in diabetes, one may have to consider the diabetic model, the injury model and the gender of the rats as well as determine e.g.…”

Section: Discussionmentioning

confidence: 99%

Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats

Stenberg

Dahlin

2014

BMC Neurosci

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BackgroundIn view of the global increase in diabetes, and the fact that recent findings indicate that diabetic neuropathy is more frequently seen in males, it is crucial to evaluate any gender differences in nerve regeneration in diabetes. Our aim was to evaluate in short-term experiments gender dissimilarities in axonal outgrowth in healthy and in genetically developed type 2 diabetic Goto-Kakizaki (GK) rats, and also to investigate the connection between activated (i.e. ATF-3, Activating Transcription Factor 3) and apoptotic (cleaved caspase 3) Schwann cells after sciatic nerve injury and repair. Female and male diabetic GK rats, spontaneously developing type 2 diabetes, were compared with corresponding healthy Wistar rats. The sciatic nerve was transected and instantly repaired. After six days the nerve was harvested to measure axonal outgrowth (i.e. neurofilament staining), and to quantify the number of ATF-3 (i.e. activated) and cleaved caspase 3 (i.e. apoptotic) stained Schwann cells using immunohistochemistry.ResultsAxonal outgrowth was generally longer in male than in female rats and also longer in healthy than in diabetic rats. Differences were observed in the number of activated Schwann cells both in the distal nerve segment and close to the lesion site. In particular the female diabetic rats had a lower number. There were no gender differences in number of cleaved caspase 3 stained Schwann cells, but rats with diabetes exhibited more (such cleaved caspase 3 stained Schwann) cells both at the lesion site and in the distal part of the sciatic nerve. Axonal outgrowth correlated with the number of ATF3 stained Schwann cells, but not with blood glucose levels or the cleaved caspase 3 stained Schwann cells. However, the number of cleaved caspase 3 stained Schwann cells correlated with the blood glucose level.ConclusionsWe conclude that there are gender differences in nerve regeneration in healthy rats and in type 2 diabetic GK rats.

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Section: Discussionmentioning

confidence: 99%

Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats

Stenberg

Dahlin

2014

BMC Neurosci

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“…insulin resistance and impaired glucose tolerance) increase the susceptibility to nerve compression [13,14]. Previous studies [15][16][17], though not all [18], state that patients with DM benefit from CTR to the same extent as patients without DM.…”

Section: Diabetesmentioning

confidence: 99%

Outcome after carpal tunnel release: impact of factors related to metabolic syndrome

Zimmerman

Dahlin

Thomsen

et al. 2016

Journal of Plastic Surgery and Hand Surgery

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165-171. DOI: 10.1080165-171. DOI: 10. /2000656X.2016 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. In a retrospective observational study, we evaluated outcome after open carpal tunnel release focusing on factors related to the metabolic syndrome: diabetes, hypertension, obesity (BMI ≥ 30) and statin treatment. MethodsResults from 493 out of 962 patients (531/1044 hands) operated for CTS during 18 months that had filled in QuickDASH questionnaires before and one year after surgery were included in the study. ResultsPatients with diabetes (n=76) p<0.05). The odds of having a change in QuickDASH score <8 was 2.6 times higher in patients with polyneuropathy than in patients without polyneuropathy.Patients with hypertension, obesity or statin treatment had a similar improvement after surgery as patients without these factors. ConclusionsPatients with diabetes without neuropathy, as well as patients with hypertension, obesity or statin treatment, and CTS can expect the same effects of open carpal tunnel release as otherwise healthy patients. Patients with diabetic neuropathy and CTS did not experience the 3 same improvement as otherwise healthy patients and should be informed about the risk of an unsatisfactory outcome.Word count abstract: 242

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“…However, Middlemas et al [7] did not find any altered anterograde transport p-ERK 1/2 in STZinduced diabetes, but only observed increased levels of retrogradely transported c-Jun N-terminal kinase and p38 (members of the stress-activated protein kinase family together with p-ERK 1/2). Interestingly, a slighter and sustained trauma, such as a nerve compression lesion, in a sciatic nerve in the spontaneously developing diabetes in BB and Goto-Kakizaki-rats (GK; type 2 diabetes) induces a more pronounced regeneration-related response (upregulation of the activating transcription factor 3) than in healthy rats with an identical compression lesion [13]. Thus, perturbed signal transduction mechanisms by diabetes may result in increased activation of some pathways and suppression of others.…”

Section: Discussionmentioning

confidence: 97%

Injury-induced activation of ERK 1/2 in the sciatic nerve of healthy and diabetic rats

Stenberg

Kanje²,

Mårtensson³

et al. 2011

NeuroReport

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Phosphorylation of extracellular-signal-regulated kinase 1/2 (p-ERK 1/2) was investigated by immunohistochemistry at 30 min, 1 h, and 48 h after nerve transection in the sciatic nerve of healthy and diabetic [streptozotocin (STZ)-induced diabetes mellitus and BioBreeding (BB; i.e. DR.lyp/lyp or BBDP)] rats. Transection injury increased the intensity of p-ERK 1/2 in nerve stumps at all time points. Staining was confined to Schwann cells with occasional faint staining in single axons. In diabetic rats, a lower intensity of p-ERK 1/2 was found at 1 and 48 h in the distal and proximal nerve stumps compared with healthy rats. STZ-induced diabetic rats were not different from BB rats. p-ERK 1/2 is activated differentially in Schwann cells after nerve injury in diabetic rats, whereas activation in STZ-induced diabetic rats did not differ from BB rats.

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Nerve compression induces activating transcription factor 3 in neurons and Schwann cells in diabetic rats

Cited by 22 publications

References 23 publications

Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats

Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats

Outcome after carpal tunnel release: impact of factors related to metabolic syndrome

Injury-induced activation of ERK 1/2 in the sciatic nerve of healthy and diabetic rats

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