Nerve cells culture from lumbar spinal cord on surfaces modified by plasma pyrrole polymerization

Abstract

Currently, there are several techniques for modified cell culture surfaces under research to improve cell growth and adhesion. Recently, different methods have been used for surface coating, using biomolecules that enhance cell attachment and growth of nerve cells from spinal cord, such as the use of Poly-DL-Ornithine/Laminin. Plasma-polymerized pyrrole (PPy)-treated surfaces have showed improvement on surfaces biocompatibility with the cells in culture since they do not interfere with any of the biological cell functions. In the present work, we present a novel mouse nerve cell culture technique, using PPy-treated cell culture surfaces. A comparative study of cell survival using Poly-DL-Ornithine/Laminin-treated surfaces was performed. Our results of cell survival when compared with data already reported by other investigators, show that cells cultured on the PPy-modified surface increased survival up to 21 days when compared with Poly-DL-Ornithine/Laminin-coated culture, where 8 days cell survival was obtained. There were electrical and morphological differences in the nerve cells grown in the different surfaces. By comparing the peak ion currents of Poly-DL-Ornithine/Laminin-seeded cells for 8 days with cells grown for 21 days on PPy, an increase of 516% in the Na(+) current and 127% in K(+) currents in cells seeded on PPy were observed. Immunofluorescence techniques showed the presence of cell synapses and culture viability after 21 days. Our results then showed that PPy-modified surfaces are an alternative culture method that increases nerve cells survival from lumbar spinal cord cell culture by preserving its electrical and morphological features.

Modeling integrin and plasma-polymerized pyrrole interactions: chemical diversity relevance for cell regeneration

“…The plasma reactor is described in a previous work 17 , is formed of a pyrex glass tube of 25 cm length, 9 cm of external diameter and 5 mm thickness, each end of the tube is sealed by a stainless steel lids and in each lid there are two access ports and one central access, where one electrode for plasma discharge is introduced. In a lid and using the access ports, a pirani probe (Edwards) is placed to measure the pressure inside the discharge chamber, by the other access a vacuum system is connected.…”

“…Among other methods, the polypyrrole synthesized by plasma (PPPy) and iodine-doped (PPPy-I) exhibit an extraordinary capacity as culture cell support. Different cell types have been seeded and proliferated on biocompatible scaffolds coated by PPPy 16,17 . One of the most important applications is based on the implementation of mesoparticles of PPPy doped with iodine for spinal cord injury treatment.…”

Plasma Functionalized Scaffolds of Polyhydroxybutyrate Electrospun Fibers for Pancreatic Beta Cell Cultures

“…One technique that has been shown to be effective in modifying the surface of different polymers is plasma polymerization. Surface modification can be accomplished by depositing a polymeric thin film that has functional groups that allow biomolecules to anchor to the surface (Ramírez-Fernández et al, 2012;Zuñiga Aguilar et al, 2014). Plasma polymerization using pyrrole as monomer has shown reliable performance in producing surfaces that promote cell adhesion and proliferation, especially in in vivo experiments (Ramírez-Fernández et al, 2015;Ruiz Velasco et al, 2017).…”

Effect of different exposure times on physicochemical, mechanical and biological properties of PGS scaffolds treated with plasma of iodine-doped polypyrrole

“…14 This improvement has been associated with the chemical species that contains N-H bond, which has been proven to stimulate cell growth. 15–17 In this regard, Mejía et al. 18 and Olayo et al., 13 reported that PPy-Mesoparticles, when administered directly into the nerve tissues of rats with spinal cord injury, were successfully integrated into these nerve cells without inducing an acute inflammatory response.…”