Nephrops norvegicus: field study of effects of oxygen deficiency on haemocyanin concentration

Hagerman, Lars; Baden, Susanne

doi:10.1016/0022-0981(88)90051-2

Cited by 45 publications

(26 citation statements)

References 8 publications

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“…As well as density-dependent effects, populations of Nephrops norvegicus are also subject to a variety of environmental stresses including trawling pressure, prolonged exposure to hypoxia (Hagerman & Baden 1988, Baden et al 1994) and parasitic infection (Field et al 1992, 1995, Taylor et al 1996. Convenient methods of evaluating the condition of recently captured N. norvegicus, such as nucleic acid indices, may therefore provide a valuable tool for the assessment of their nutritional status, and may contribute to stock-management decisions.…”

Section: Discussionmentioning

confidence: 99%

Nucleic acids as indicators of nutritional condition in the Norway lobster Nephrops norvegicus

Parslow-Williams¹,

Atkinson²,

Taylor³

2001

Mar. Ecol. Prog. Ser.

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Changes in nucleic acid concentration of the gill, hepatopancreas and abdominal muscle tissue of the Norway lobster Nephrops norvegicus (L.) were examined after 6 and 12 wk of starvation. Significant decreases in RNA concentration, RNA:protein ratio and RNA:DNA ratio were recorded in abdominal muscle tissue following starvation, but not in gill or hepatopancreas tissue. Samples of abdominal muscle tissue were taken from lobsters from 2 sites in the Clyde Sea area, Scotland. These were south of Ailsa Craig, where N. norvegicus are thought to be nutritionally limited as a result of the high population densities that occur in this area, and south of Little Cumbrae island, where population density is lower. Evidence of nutritional limitation was provided by nucleic acid analysis which showed that the RNA concentration, RNA:protein ratio and RNA:DNA ratio of lobsters from south of Ailsa Craig were significantly lower than in lobsters of equivalent size from south of Little Cumbrae. Further studies demonstrated that animals from each site were capable of obtaining nucleic acid levels similar to those at the other site after a few weeks when exposed to different feeding regimes.

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Section: Discussionmentioning

confidence: 99%

Nucleic acids as indicators of nutritional condition in the Norway lobster Nephrops norvegicus

Parslow-Williams¹,

Atkinson²,

Taylor³

2001

Mar. Ecol. Prog. Ser.

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“…Since the reported concentrations of hemolymph protein of penaeids range from 62 to 103 mg ml-l (Bursey & Lane 1971, Balazs et al 1974, dilution of hemolymph sample is necessary. This dilution technique has been used successfully for determinations of hemocyanin and protein in a number of decapod crustaceans (Senkbeil & Wriston 1981, Hagerman 1983, 1986, Ferraris et al 1986, Hagerman & Baden 1988. To check if dilution affected the measured concentrations of hemocyanin and protein, the hemolymph samples were allowed to coagulate at 4 "C, and then centrifuged at 10 000 X gfor 10 min.…”

Section: Penaeus Monodon Obtained From a Shrimp Farm Inmentioning

confidence: 99%

Nitrogen excretion and changes of hemocyanin, protein and free amino acid levels in the hemolymph of Penaeus monodon exposed to different concentrations of ambient ammonia-N at different salinity levels

Chen¹,

Chen²,

Cheng³

1994

Mar. Ecol. Prog. Ser.

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ABSTRACT. Penaeus monodon (22.064 * 2.263 g) were exposed individually at 10, 20 and 30% salinity to 0 (control), 1, 5, 10 and 20 mg 1-' ammonia-N for 24 h. Changes in hemocyanin, protein and free amino acid levels, and whole shrimp ammonia-N excretion, urea-N excretion and dissolved organic N (DON) excretion were determined. Ammonia-N excretion decreased with increased salinity and with increased ambient ammonia-N. Ammonia-N accounted for 56.87. 70.43 and 78.13 % of total nitrogen excreted by P. monodon in 30, 20 and 10% respectively. DON increased to 67.26, 78.79 and 86.1% of total nitrogen excreted by the shrimp exposed to 20 mg 1-' ammonia-N in 30, 20 and 10°A, respectively. Net ammonia-N uptake occurred as shrimp were exposed to ambient ammonia-N greater than 5 mg 1-I at all salinity levels tested. Hemocyanin and protein levels decreased, whereas hemolyrnph ammonia, urea, total free amino acid levels and taurine level increased, with increased ambient ammonia-N. Following exposure to ambient ammonia-N, P. rnonodon changed its excretory pattern, accumulation of hemolymph ammonia, and catabolism of hemocyanin and protein to balance osmoregulation.

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“…Nephrops norvegicus exposed to prolonged hypoxia in situ were found to have lowered levels of haemocyanin in the blood. This was attributed to starvation resulting from reduced feeding at low environmental oxygen tensions (Hagerman & Pihl Baden, 1988),…”

Section: Haemocyanin Concentration and Environmental Fluctuationsmentioning

confidence: 99%

Haemolymph protein composition and copper levels in decapod crustaceans

Depledge¹,

Bjerregaard²

1989

Helgolander Meeresunters

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Variations in haemolymph protein composition and concentration, in copper content and copper distribution in the tissue of decapod crustaceans are reviewed. Haemocyanin is the major haemolymph constituent (> 60 %); the remaining proteins (in order of concentration) incIude coagulogen, apohaemocyanin, hormones and antisomes. Moulting, nutritional state, infection, hypoxia and salinity fluctuations are the major factors affecting the relative proportions and total quantities of the haemolymph proteins. With regard to haemocyanin, the changes in concentration during the moult cycle are principally associated with changes in haemolymph volume, rather than with changes in total haemocyanin content due to synthesis or catabolism. The role of the midgut gland in regulating haemolymph copper and haemocyanin concentration has been re-evaluated. More than 50 % of the whole body copper load is stored in the haemolymph. In contrast, less than 3 % of the copper load resides in the midgut gland. The latter has little potential for regulating haemolymph copper levels, at least in the short term (hours to a few days), though it may be involved in regulating haemocyanin levels over longer periods (weeks to months). The total coppe r content of the haemolymph remains within a narrow range, except during starvation when levels may decrease. Consequently, variations in the copper content of soft tissues, which constitute only 20 % of decapod dry weight, do not significantly alter whole body copper concentrations. Evidence that copper released following haemocyanin catabolism becomes bound to metallothionein for later use in the resynthesis of haemocyanin is reviewed and found to be inqonclusive. The amount of copper that can be stored in this way is trivial compared with the amount of copper required to permit significant changes in haemolymph haemocyanin concentration. Average tissue copper requirements, calculated during the present study, are approx. 4 times higher than previous theoretical estimates.

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Nephrops norvegicus: field study of effects of oxygen deficiency on haemocyanin concentration

Cited by 45 publications

References 8 publications

Nucleic acids as indicators of nutritional condition in the Norway lobster Nephrops norvegicus

Nucleic acids as indicators of nutritional condition in the Norway lobster Nephrops norvegicus

Nitrogen excretion and changes of hemocyanin, protein and free amino acid levels in the hemolymph of Penaeus monodon exposed to different concentrations of ambient ammonia-N at different salinity levels

Haemolymph protein composition and copper levels in decapod crustaceans

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