Nephroprotective effect of Artemisia herba alba aqueous extract in alloxan-induced diabetic rats

Abstract

Background and aim In the present study, we investigate the phytochemical composition and the nephroprotective effects as well as the antioxidant properties of Artemisia herba alba aqueous extract in alloxan-induced experimental diabetes in rats. Experimental procedure Wistar rats were divided into four groups of seven rats each: Group I: Normal control (NC) received saline solution at 9‰ given by intraperitoneal way; Group II: Diabetic control (DC) received alloxan (150 mg/kg b.w) intraperitoneally; Group III: Normal control (NC + AHA) received saline solution at 9‰ and treated orally by AHA aqueous extract (400 mg/kg/b.w); Group IV: Diabetic control (DC + AHA) received alloxan solution (150 mg/kg b.w) intraperitoneally and treated by aqueous extract of AHA (400 mg/kg/b.w/day) orally after one week of alloxan administration. After 30 days, blood and tissue samples were collected for biochemical and histopathological analysis, respectively. Glomerular damage markers, including creatinine, serum urea, urine creatinine and urine urea levels were estimated. Creatinine clearance was also assessed. Oxidative stress parameters were assessed in the kidney homogenate. Results and conclusion Alloxan-exposure resulted in significant increase in blood glucose and serum level of glomerular damage markers. The antioxidant enzyme activities were significantly downregulated associated with an increase in malondialdehyde (MDA) level over the baseline values. Artemisia herba alba aqueous extract supplementation significantly improved the studied parameters. In concluding, the results obtained suggests that Artemisia herbs-alba aqueous extract supplementation reduces alloxan-induced free radical generation, potentiates the antioxidant defense system and alleviates renal sensitivity to oxidative stress.

Hepatoprotective and Nephroprotective Activity of Artemisia absinthium L. on Diclofenac-induced Toxicity in Rats

“…15 Furthermore, the nephroprotective effect of A. absinthium has not been previously studied, and the greater effect of methanolic extract has been demonstrated by this study. Although the nephroprotective effect of A. absinthium has not been previously studied, the effect of other Artemisia species has been studied, showing the effect of Artemisia arborescens 30 , Artemisia herba-alba 31 , Artemisia sieberi 12 , Artemisia campestris L. 32 and Artemisia scoparia 33 , being similar to the one presented by A. absinthium. At the histopathological level, the methanolic extract of A. absinthium showed a substantial decrease in tubular and glomerular damage, becoming physiologically almost normal.…”

In-Silico Identification of Potent Inhibitors of COVID-19 Main Protease (Mpro) and Angiotensin Converting Enzyme 2 (ACE2) from Natural Products: Quercetin, Hispidulin, and Cirsimaritin Exhibited Better Potential Inhibition than Hydroxy-Chloroquine Against COVID-19 Main Protease Active Site and ACE2

“…Hispidulin, Cirsimaritin, and Artemisin are the main flavonoids isolated from Artemesia herba alba (Shen et al, 1994). Artemesia herba alba displays a large variety of biological activities including antidiabetic, antihyperlipidemic and nephroprotective (Sekiou et al, 2020, Sekiou et al, 2019, cardioprotective (Irshaid et al,2012), anticancer (Khlifi et al,2013), antioxidant (Sekiou et al, 2019), antiprotozoal (Bachrouch et al, 2015), gastroprotective (LAOUFI et LADJ, 2017), antibacterial (Yashphe et al, 1987), antihepatotoxic (Sekiou et al, 2019), insecticidal (Sharifian et al, 2012), Essential oils of Artemesia herba alba have also antihypertensive activities (Zeggwagh et al, 2008). Sulfasalazine [Salazopyrin®] is an intestinal antiinflammatory, developed in the 1950s to treat rheumatoid arthritis (Taffet et al, 1983).…”

Protective effect of Artemisia herba-alba extract on the liver of diabetic albino male rats

“…There is a recent study trying to detect the nephroprotective effect of Artemisia herba-alba aqueous extract in alloxan-induced diabetic rats [2] and prolonged protective effects of Artemisia herba-alba extracts [4].The main goal of the present study is to evaluate the protective effect of Artemisia herba-alba extract on the liver in STZinduced diabetic male rats.…”

“…Diabetes is a metabolic disorder characterized by chronic hyperglycemia due to impaired regulation of carbohydrate metabolism due to impaired insulin secretion or impaired action of this hormone. Chronic hyperglycemia is associated with the development of serious long-term complications such as cardiovascular disease, chronic renal failure, and retinal damage [2]. Currently available anti-diabetes drugs include sulfonylureas, biguanides, thiazolidinediones, and αglucosidase inhibitors and are vastly used to control hyperglycemia.…”