Nephroprotective and Anti-Inflammatory Effects ofPistacia atlanticaLeaf Hydroethanolic Extract Against Gentamicin-Induced Nephrotoxicity in Rats

Abstract

Gentamicin in overdose can lead to tubular injury and kidney dysfunction. Some antioxidants can protect kidneys against nephrotoxicity. This study was undertaken to evaluate the protective effects of Pistacia atlantica (P. atlantica) leaf hydroethanolic extract against gentamicin-induced nephrotoxicity in rats. Forty rats were divided into five groups: the first group received a daily intraperitoneal (i.p.) injection of normal saline. The second group received gentamicin (120 mg/kg, i.p.). The third, fourth, and fifth groups were orally treated with 200, 400, and 800 mg/kg of P. atlantica leaf hydroethanolic extract, respectively, and they also received gentamicin (120 mg/kg, i.p.). After seven days, serum malondialdehyde (MDA), creatinine (Cr), urea, uric acid, lipids profile, protein carbonyl (PC), and tumor necrosis factor-α (TNF-α) were determined. Also, a piece of kidney was used to determine catalase (CAT) and superoxide dismutase (SOD) activities, vitamin C, the gene expression of TNF-α, and for subsequent histopathological studies. Treatment with P. atlantica leaf hydroethanolic extract resulted in a significant increase (p < 0.05) in CAT, SOD, vitamin C, and high-density lipoprotein cholesterol, and significantly decreased (p < 0.05) the levels of Cr, urea, uric acid, MDA, PC, triglyceride, total cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, TNF-α protein, and the gene expression of TNF-α compared with the untreated group. Histopathological studies show that in lymphocyte infiltration, remarkable reduction was observed in P. atlantica leaf hydroethanolic extract-treated groups, compared with the untreated group. The present study suggests that P. atlantica leaf hydroethanolic extract has protective effects against gentamicin-induced nephrotoxicity.

Ameliorative effects ofN‐acetyl cysteine on diclofenac‐induced renal injury in male rats based on serum biochemical parameters, oxidative biomarkers, and histopathological study

“…TNF‐α leads to oxidative stress which in turn increases the renal cells damage (Elmarakby & Sullivan, ; Ziamajidi, Nasiri, Abbasalipourkabir, & Sadeghi Moheb, ). Also, previous findings demonstrated that TNF‐α gene expression is elevated in renal damages (Heidarian et al, ; Valipour et al, ). In our research, administration of DIC caused an elevation in the renal expression of TNF‐α and its serum level (Figure ) which was accompanied with infiltration of lymphocytes in the renal section of DIC‐only‐treated group (Figure ).…”

“…Produced ROS resulting from DIC can ruin lipids and proteins. Also, MDA and PC can result from protein oxidation and fatty acid peroxidation (Heidarian, Jafari‐Dehkordi, Valipour, Ghatreh‐Samani, & Ashrafi‐Eshkaftaki, ). In this study, administration of the DIC led to oxidative stress in the kidney which in turn causes nephrotoxicity.…”

应用动物模型探索肾脏疾病作用机制和潜在治疗靶点

“…Nevertheless, nephrotoxicity limits its use in clinical practice ( He et al, 2015 ). Doses of gentamicin ranging from 40–200 mg/kg administered for 4–10 d ( Bledsoe et al, 2008 ; Boroushaki et al, 2014 ; Heidarian et al, 2017 ; Jabbari et al, 2011 ) can induce acute renal failure in rats. Administration of 100 mg/kg i.p.…”

<p>Antihyperlipidemic and Antioxidative Properties of <em>Pistacia atlantica</em> subsp. <em>kurdica</em> in Streptozotocin-Induced Diabetic Mice</p>

“…11 It has been shown that P. atlantica and its chemical factors induce some pharmacological actions including anticancer, antioxidant, anti-inflammatory, and antimicrobial activities. [12][13][14][15] Therefore, this project was initiated to evaluate the impact of long-term treatment with hydroalcoholic extract (HE) and essential oil (EO) of P. atlantica subsp. kurdica on glucose, lipid profile, TNF-α and oxidative stress biomarkers in streptozotocin (STZ)induced diabetic mice.…”