Nephroprotective activity of Combretum micranthum G. Don in cisplatin induced nephrotoxicity in rats: In-vitro, in-vivo and in-silico experiments

Kpemissi, Mabozou; Eklu-Gadégbéku, Kwashie; Veerapur, Veeresh P.; Negru, Mihai; Taulescu, Marian; Chandramohan, Vivek; Hiriyan, Jagadheshan; Banakar, Siddalingesh M.; Nv, Thimmaiah; Suhas, Doddamavattur Shivalingaiah; Puneeth, Tumbadi Adinarayanashetty; Vijayakumar, Sachidananda; Metowogo, Kossi; Aklikokou, Kodjo

doi:10.1016/j.biopha.2019.108961

Cited by 35 publications

(19 citation statements)

References 40 publications

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“…It was previously evidenced that the presence of polyphenols and flavonoids in the plant extract will protect the tissues from being oxidised and prevent injury 12 . In Abrus precatorious chloroform extract also after performing phytochemical screening, the results evidenced the presence of polyphenols and flavonoids.…”

Section: Fig 1: Graph Representing Serum Creatininementioning

confidence: 99%

Untitled

2021

IJPSR

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Section: Fig 1: Graph Representing Serum Creatininementioning

confidence: 99%

Untitled

2021

IJPSR

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“…Antioxidant and antiapoptotic properties of plant extracts were confirmed by in vitro investigations performed on renal tubular epithelia cells [ 100 ] and cardiomyocytes [ 25 ]. Histopathological changes were also found to be relieved by plant materials [ 16 , 23 , 24 , 26 , 102 ].…”

Section: The Protective Properties Of Plant Preparations Against Tmentioning

confidence: 99%

The Great Healing Potential Hidden in Plant Preparations of Antioxidant Properties: A Return to Nature?

Kiełczykowska

Musik

2020

Oxidative Medicine and Cellular Longevity

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The application of chemicals in industry and agriculture has contributed to environmental pollution and exposure of living organisms to harmful factors. The development of new pharmaceutical agents enabled successful therapy of various diseases, but their administration may be connected with side effects. Oxidative stress has been found to be involved into etiology of numerous diseases as well as harmful action of drugs and chemicals. For some time, plant origin substances have been studied as potential protective agents alleviating toxicity of various substances and symptoms of diseases. The aim of the current review was to present the diversity of the research performed during the last five years on animal models. The outcomes showed a huge protective potential inherent in plant preparations, including alleviating prooxidative processes, strengthening antioxidant defence, ameliorating immune parameters, and reversing histopathological changes. In many cases, plant origin substances were proved to be comparable or even better than standard drugs. Such findings let us suggest that in the future the plant preparations could make adjuvants or a replacement for pharmaceutical agents. However, the detailed research regarding dose and way of administration as well as the per se effects needs to be performed. In many studies, the last issue was not studied, and in some cases, the deleterious effects have been observed.

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“…The operation was followed by running in 150ns NPT production simulation in triplicate to confirm that protein was not stuck in local minima. After the molecular dynamics simulation, the structural changes in the monomer COPS2 protein molecule and mutant's proteins were analyzed through RMSD, RMSF, SSE, Radius of gyration (Rg) and Hydrogen bonding [34][35][36].…”

Section: Molecular Dynamicsmentioning

confidence: 99%

Finding Pathogenic nsSNP’s and their structural effect on COPS2 using Molecular Dynamic Approach

Malik¹,

Pande²,

Kumar³

et al. 2020

Preprint

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COP9 Signalosome Subunit 2 is a highly conserved multiprotein complex which is involved in the cellular process and developmental process. It is one of the essential components in the COP9 Signalosome Complex (CSN). It is also involved in neuronal differentiation interacting with NIF3L1. The gene involved in neuronal differentiation is negatively regulated due to the transcription co-repressor interaction of NIF3L1 with COPS2. In the present study, we have evaluated the outcome for 90 non-synonymous single nucleotide polymorphisms (nsSNPs) in COPS2 gene through computational tools. After the analysis, 4 SNPs (S120C, N144S, Y159H, R173C) were found to be deleterious. The native and mutated structures were prepared using discovery studio and docked to check the interactions with NIF3L1.On the basis of ZDOCK score the top 3 mutations (N144S, Y159H, R173C) were screened out. Further to analyze the effect of amino acid substitution on the molecular structure of protein Molecular Dynamics simulation was carried out. Analysis based on RMSD, RMSF, RG, H-bond showed a significant deviation in the graph, which demonstrated conformation change and instability compared to the wild structure. As it is known mutations in COPS2 gene can disrupt the normal activity of the CSN2 protein which may cause neuronal differentiation. Our results showed N144S, Y159H and R173C mutations are to be more pathogenic and may cause disease.

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Nephroprotective activity of Combretum micranthum G. Don in cisplatin induced nephrotoxicity in rats: In-vitro, in-vivo and in-silico experiments

Cited by 35 publications

References 40 publications

Untitled

Untitled

The Great Healing Potential Hidden in Plant Preparations of Antioxidant Properties: A Return to Nature?

Finding Pathogenic nsSNP’s and their structural effect on COPS2 using Molecular Dynamic Approach

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