Nephropathia epidemica with a 6-week incubation period after occupational exposure to Puumala hantavirus

Kramski, Marit; Achazi, Katharina; Klempa, Boris; Krüger, Detlev H.

doi:10.1016/j.jcv.2008.10.005

Cited by 24 publications

(15 citation statements)

References 14 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The symptoms of PUUV infection, nephropathia epidemica (NE, a mild form of HFRS), include abrupt fever, followed by headache, nausea, vomiting, abdominal pain, and signs of renal insufficiency; somnolence and visual disturbances are common, and pulmonary, cardiac, and central nervous system symptoms have also been recorded. The incubation period is 2 to 6 weeks (5)(6)(7)(8). Hantavirus infection induces a strong humoral IgM and IgG antibody response against the structural proteins, particularly the nucleocapsid (N) protein in the acute phase.…”

mentioning

confidence: 99%

Rapid Homogeneous Immunoassay Based on Time-Resolved Förster Resonance Energy Transfer for Serodiagnosis of Acute Hantavirus Infection

Hepojoki

Hedman

et al. 2015

J Clin Microbiol

View full text Add to dashboard Cite

We recently introduced a homogeneous immunoassay based on time-resolved Förster resonance energy transfer (TR-FRETP uumala virus (PUUV) belongs to the genus Hantavirus within the family Bunyaviridae, comprising typically rodent-and insectivore-borne negative-stranded RNA viruses (1). The known reservoir hosts of hantaviruses are chronically infected and spread the virus via excreta (saliva, urine, and feces) (2). The human infection, acquired via inhalation of aerosolized rodent excreta, manifests as two disease entities: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). HFRS predominates in Eurasia, and HCPS occurs in the Americas. The case fatality rate of HFRS is 0.1 to 12%, depending on the virus type, and that of HCPS is up to 40% (3).Annually, around 50,000 hospitalizations due to HFRS occur in Eurasia, while a total of ϳ3,500 HCPS cases have been recorded since 1993 (4). In Europe, the most common cause of HFRS (up to 10,000 cases per annum) is PUUV that circulates in bank voles (Myodes glareolus) (5). The symptoms of PUUV infection, nephropathia epidemica (NE, a mild form of HFRS), include abrupt fever, followed by headache, nausea, vomiting, abdominal pain, and signs of renal insufficiency; somnolence and visual disturbances are common, and pulmonary, cardiac, and central nervous system symptoms have also been recorded. The incubation period is 2 to 6 weeks (5-8). Hantavirus infection induces a strong humoral IgM and IgG antibody response against the structural proteins, particularly the nucleocapsid (N) protein in the acute phase. Laboratory diagnosis of hantavirus disease is based on the detection of IgM and IgG antibodies, usually by immunochromatography (IgM), enzyme immunoassay (EIA) with recombinant N as the antigen, or immunofluorescence assay (IFA) based on acetone-fixed infected cells (9-11).Förster resonance energy transfer (FRET) has been applied widely in biomedical research (12)(13)(14)(15)(16)(17)(18)(19), and our goal is to harness the phenomenon for infectious disease serodiagnostics. In FRET, an excited donor fluorophore transfers its energy to an acceptor fluorophore in close proximity (Ͻ10 nm). Time-resolved FRET (TR-FRET) utilizes the long fluorescence emission half-lives of lanthanides and enables background-free measurement from biological materials. We recently introduced two different principles utilizing TR-FRET for antibody detection. The first approach is based on binding of an immunoglobulin molecule to its antigens labeled distinctly with donor and acceptor fluorophores, giving rise to a FRET signal in a homogenous (wash-free) assay format (12). The second approach is based on binding of an immunoglobulin molecule to its antigen and protein L (a bacterial protein targeting the Ig kappa light chain) labeled distinctly with fluorophores forming a FRET pair (20). The emitted TR-FRET signal is proportional to the amount of specific immunoglobulin. Here, using recombinant full-length N protein of PUUV (PUUV-N), we showed that the protein...

show abstract

mentioning

confidence: 99%

Rapid Homogeneous Immunoassay Based on Time-Resolved Förster Resonance Energy Transfer for Serodiagnosis of Acute Hantavirus Infection

Hepojoki

Hedman

et al. 2015

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…Typical symptoms of NE include abrupt fever, followed by headache and nausea, vomiting, abdominal pain, and signs of renal insufficiency. Pulmonary, cardiac, and central nervous system symptoms, as well as somnolence and visual disturbances, are also common (2)(3)(4)(5). Laboratory diagnosis of PUUV infection is based on the detection of immunoglobulin M (IgM) to PUUV or low avidity of immunoglobulin G (IgG).…”

mentioning

confidence: 99%

Competitive Homogeneous Immunoassay for Rapid Serodiagnosis of Hantavirus Disease

et al. 2015

View full text Add to dashboard Cite

In this study, we describe a competitive homogeneous immunoassay that makes use of Förster resonance energy transfer (FRET) in rapid detection of pathogen-specific antibodies. The assay principle is based on competition between a monoclonal antibody (MAb) and serum antibodies to a given antigen. In the assay, named competitive FRET immunoassay (CFRET-IA), the FRET signal is induced if MAb carrying a donor label binds to an acceptor-labeled antigen. Specific antibodies in serum compete for antigen binding, resulting in reduced FRET signal. The proof-of-principle for the assay was obtained using donor-labeled Puumala virus nucleocapsid protein (PUUV-N) and acceptor-labeled anti-PUUV-N MAb. The assay was evaluated by analyzing 329 clinical samples comprising 101 from individuals with acute PUUV infection, 42 from individuals with past infection, and 186 from individuals with PUUV-seronegative sera, and the results were compared to those of reference tests. The rapid serodiagnostic test we introduced herein performed with 100% sensitivity and 99% specificity for diagnosing acute hantavirus disease.

show abstract

“…The clinical picture and severity differ between HFRS and HCPS but basic pathogenesis is believed to be caused by increased vascular permeability. The incubation period is normally at least 2 weeks but can be up to 6 weeks or longer [4,132,133], suggesting that hantaviruses replicate for a prolonged period before onset of HFRS/HCPS. The long incubation period observed for HFRS/HCPS, the fact that not all infected individuals develop disease and that infection of the natural host seems to be subclinical, suggest that viral replication per se does not normally cause disease.…”

Section: Pathogenesis: Why Do Hantaviruses Cause Disease In Humans?mentioning

confidence: 99%

Hantavirus protein interactions regulate cellular functions and signaling responses

Klingström

Ahlm

2011

Expert Review of Anti-infective Therapy

View full text Add to dashboard Cite

Nephropathia epidemica with a 6-week incubation period after occupational exposure to Puumala hantavirus

Cited by 24 publications

References 14 publications

Rapid Homogeneous Immunoassay Based on Time-Resolved Förster Resonance Energy Transfer for Serodiagnosis of Acute Hantavirus Infection

Rapid Homogeneous Immunoassay Based on Time-Resolved Förster Resonance Energy Transfer for Serodiagnosis of Acute Hantavirus Infection

Competitive Homogeneous Immunoassay for Rapid Serodiagnosis of Hantavirus Disease

Hantavirus protein interactions regulate cellular functions and signaling responses

Contact Info

Product

Resources

About