Nephrinuria and podocytopathies

Kostovska, Irena; Tosheska-Trajkovska, Katerina; Topuzovska, Sonja; Cekovska, Svetlana; Labudovic, Danica; Костовски, Огнен; Spasovski, Goce

doi:10.1016/bs.acc.2021.08.001

Cited by 5 publications

(6 citation statements)

References 137 publications

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“…Foot processes are composed of several proteins, of which nephrin is the earliest identified and most studied. Nephrin is an essential component of podocytes which combines with endothelial cells and the basement membrane to form the glomerular filtration barrier [ 34 ]. Podocyte damage results in nephrin release.…”

Section: Discussionmentioning

confidence: 99%

Iron Chelator Deferoxamine Alleviates Progression of Diabetic Nephropathy by Relieving Inflammation and Fibrosis in Rats

Feng,

Jia,

et al. 2023

Biomolecules

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Diabetic nephropathy (DN) is one of the most devastating diabetic microvascular complications. It has previously been observed that iron metabolism levels are abnormal in diabetic patients. However, the mechanism by which iron metabolism levels affect DN is poorly understood. This study was designed to evaluate the role of iron-chelator deferoxamine (DFO) in the improvement of DN. Here, we established a DN rat model induced by diets high in carbohydrates and fat and streptozotocin (STZ) injection. Our data demonstrated that DFO treatment for three weeks greatly attenuated renal dysfunction as evidenced by decreased levels of urinary albumin, blood urea nitrogen, and serum creatinine, which were elevated in DN rats. Histopathological observations showed that DFO treatment improved the renal structures of DN rats and preserved podocyte integrity by preventing the decrease of transcripts of nephrin and podocin. In addition, DFO treatment reduced the overexpression of fibronectin 1, collagen I, IL-1β, NF-κB, and MCP-1 in DN rats, as well as inflammatory cell infiltrates and collagenous fibrosis. Taken together, our findings unveiled that iron chelation via DFO injection had a protective impact on DN by alleviating inflammation and fibrosis, and that it could be a potential therapeutic strategy for DN.

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Section: Discussionmentioning

confidence: 99%

Iron Chelator Deferoxamine Alleviates Progression of Diabetic Nephropathy by Relieving Inflammation and Fibrosis in Rats

Feng,

Jia,

et al. 2023

Biomolecules

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“…Biopsy specimens obtained from patients with dasatinib-related nephrotic proteinuria revealed pinocytosis; electron microscopy showed that these cases were characterized by reduced podocyte foot processes (67)(68)(69)(70)72). Podocytes are essential for the establishment of the glomerular blood urine filtration barrier, and damage to these cells leads to proteinuria (73). Nephrotic proteinuria associated with dasatinib may be attributed to the inhibition of vascular endothelial-derived growth factor (VEGF) production (74)(75)(76).…”

Section: Nephrotoxicitymentioning

confidence: 99%

Adverse reactions after treatment with dasatinib in chronic myeloid leukemia: Characteristics, potential mechanisms, and clinical management strategies

Cheng

et al. 2023

Front. Oncol.

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Dasatinib, a second-generation tyrosine kinase inhibitor, is recommended as first-line treatment for patients newly diagnosed with chronic myeloid leukemia (CML) and second-line treatment for those who are resistant or intolerant to therapy with imatinib. Dasatinib is superior to imatinib in terms of clinical response; however, the potential pulmonary toxicities associated with dasatinib, such as pulmonary arterial hypertension and pleural effusion, may limit its clinical use. Appropriate management of dasatinib-related severe events is important for improving the quality of life and prognosis of patients with CML. This review summarizes current knowledge regarding the characteristics, potential mechanisms, and clinical management of adverse reactions occurring after treatment of CML with dasatinib.

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“…To verify whether this mouse model can be used to track specific tissue EVs' delivery to target tissues in vivo, we selected lung and kidney tissues with stronger EGFP fluorescence signal in alb-CD63 Flag EGFP-mCherry f/wt mice, and kidney tissues in villin-CD63 Flag EGFP-mCherry f/wt mice for further study. To make clear the distant tissues cell types which take up those sEVs, we used IF detection to show that liver sEVs were mostly taken up by alveolar specific-cells (pulmonary surfactant protein C, SP-C expressed) in lung (Glasser et al, 2005), and podocyterelated cells (Nephrin expressed) in kidney (Kostovska et al, 2022) of the liver specific sEV tracing mice (Figure 4A). In lung tissue, there are still a few other cells types that do not express SP-C also show EGFP fluorescence, and these cell types still need to be further verified.…”

Section: The Tracing Function Of Tissue Specific Extracellular Vesicl...mentioning

confidence: 99%

Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo

Wang

Yin

et al. 2022

Front. Cell Dev. Biol.

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Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo. This mouse model is an ideal research tool for studying the secretion amount, target tissue, and functional molecule screening of EVs in specific tissues under different pathophysiological conditions. Moreover, it provides a new research method to clarify the mechanism of secreted EVs in the pathogenesis of the disease.

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Nephrinuria and podocytopathies

Cited by 5 publications

References 137 publications

Iron Chelator Deferoxamine Alleviates Progression of Diabetic Nephropathy by Relieving Inflammation and Fibrosis in Rats

Iron Chelator Deferoxamine Alleviates Progression of Diabetic Nephropathy by Relieving Inflammation and Fibrosis in Rats

Adverse reactions after treatment with dasatinib in chronic myeloid leukemia: Characteristics, potential mechanisms, and clinical management strategies

Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo

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