Nephrin and Podocalyxin - New Podocyte Proteins for Early Detection of Secondary Nephropathies

Kostovska, Irena; Tosheska-Trajkovska, Katerina; Cekovska, Svetlana; Spasovski, Goce; Labudovic, Danica

doi:10.1515/bj-2016-0003

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…In our study both nephrin and podocalyxin levels were associated with severe tubular atrophy, interstitial fibrosis and interstitial inflammation. There are several studies affirming this correlation as well as the fact that NEP levels are correlated also with albuminuria while changes in NEP excretion have been linked to podocyte injury [19]- [21]. In our study, we also reported that segmental glomerulosclerosis was significantly associated with NEP/CR ratio.…”

Section: Discussionsupporting

confidence: 83%

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Giannou,

Gakiopoulou,

Stambolliu

et al. 2024

Preprint

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Introduction: Glomerulopathy is a term used to describe a broad spectrum of renal diseases, character-ized by dysfunction of glomerular filtration barrier, especially of podocytes. Several podocyte-associated proteins have been found and proved their usefulness as urine markers of podocyte dysfunction. Two of them are nephrin (NEP) and prodocalyxin (PDC). Purpose: This study aims to evaluate the association of podocyte damage, as it is demonstrated via the concentrations of urinary proteins, with clinical and histological data from patients with several types of glomerulonephritis. Methods: We measured urine levels of two podocyte-specific markers, NEP and PDC (corrected for urine creatinine levels), in patients with a wide range of glomerulopathies. Serum and urine parameters as well as histological parameters from renal biopsy were recorded. Results: In total, data from 37 patients with glomerulonephritis and 5 healthy controls were analyzed. PDC and NEP concentrations correlated between them and with serum creatinine levels (p=0.001 and p=0.013 respectively), and with histological lesions associated with chronicity index of renal cortex, such as severe interstitial fibrosis, severe tubular atrophy and hyalinosis (for PDC/NEP, all p3gr/24h with diffuse fusion of podocyte foot processes (p=0.016) was identified. Conclusions: Podocalyxin and nephrin concentrations in urine represent markers of podocyte dysfunction and in our study they were associated both with serum creatinine and histological chronicity indices.

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Section: Discussionsupporting

confidence: 83%

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Giannou,

Gakiopoulou,

Stambolliu

et al. 2024

Preprint

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“…Moreover, IF treatment also led to structural damage to the filtration barrier, which is proved by the increased presence of another protein -NPH -in the urine of IF-treated animals. Nephrin is a structural protein of gap junctions in the foot processes of podocytes [35,36]. These visceral epithelial cells are highlyspecialized units lining the outer surface of the glomerular capillaries and that neighbor cells of the Bowman's capsule [37].…”

Section: Discussionmentioning

confidence: 99%

The Effectiveness of N-acetylcysteine in Alleviating Kidney Dysfunction in Ifosfamide-treated Rats

Dobrek¹,

Nalik-Iwaniak²,

Arent³

2020

TOUNJ

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Background: Renal damage and dysfunction are possible complications of pharmacotherapy with ifosfamide (IF), which also manifests urotoxic properties. A routine drug used to reduce the risk of IF-induced cystitis is mesna. Compounds with effect expected to be similar to mesna include N-acetylcysteine (NAC). Objective: The objective of the paper was histopathological verification of the uroprotective effect of NAC and assessment of whether this effect is accompanied by a potential nephroprotective effect. Methods: The experiment was conducted on 3 groups: 1 – control, sham-treated rats, 2 – animals treated with 5 times the IF dose administered i.p. (50 mg/kg b.w.) and 3 – rats treated with 5 times the IF dose administered i.p. + NAC administered p.o. (200 mg/kg b.w.). The renal function was evaluated analysing classical and new protein parameters (cystatin C - CysC, kidney injury molecule-1 – KIM-1 and nephrin - NPH) in blood and urine. Furthermore, histopathological analysis of bladders and kidneys was carried out. Results: Treatment with IF resulted in the development of cystitis, with no significant histopathological disturbances in the kidneys, and caused an increase in concentration and 24-hour excretion of CysC, KIM-1 NPH in the urine. Additional NAC administration caused a reduction of the said biochemical disturbances as well as improvement of the histopathological image of the urinary bladders. Conclusion: The IF therapy caused cystitis and kidney dysfunction of functional tubulopathy and early glomerulopathy character. Additional administration of NAC entailed improvement in the urinary bladder morphology and renal function. NAC is, thus, a compound exerting both uro- and nephroprotective effects.

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“…As far as the PDC/CR urine level is concerned, our study interestingly validated the correlation with activity parameters, such as severe interstitial inflammation. To the best of our knowledge, few studies have assessed both markers (PDC/CR and NEP/CR ratio) simultaneously in patients with GN [ 10 , 19 , 20 ]. A previous study assessed the impact of systemic lupus erythematosus (SLE) on urinary levels of podocalyxin, as well as its correlation with renal biopsy histological parameters, proteinuria, and disease activity [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Urine Nephrin and Podocalyxin Reflecting Podocyte Damage and Severity of Kidney Disease in Various Glomerular Diseases—A Cross-Sectional Study

Giannou,

Gakiopoulou,

Stambolliu

et al. 2024

JCM

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Background/Objectives: Glomerulopathy is a term used to describe a broad spectrum of renal diseases, characterized by dysfunction of glomerular filtration barrier, especially of podocytes. Several podocyte-associated proteins have been found and proved their usefulness as urine markers of podocyte dysfunction. Two of them are nephrin (NEP) and prodocalyxin (PDC). This study aims to evaluate the association of podocyte damage, as it is demonstrated via the concentrations of urinary proteins, with clinical and histological data from patients with several types of glomerulonephritis. Methods: We measured urine levels of two podocyte-specific markers, NEP and PDC (corrected for urine creatinine levels), in patients with a wide range of glomerulopathies. Serum and urine parameters as well as histological parameters from renal biopsy were recorded. Results: In total, data from 37 patients with glomerulonephritis and 5 healthy controls were analyzed. PDC and NEP concentrations correlated between them and with serum creatinine levels (p = 0.001 and p = 0.013 respectively), and with histological lesions associated with chronicity index of renal cortex, such as severe interstitial fibrosis, severe tubular atrophy and hyalinosis (for PDC/NEP, all p < 0.05). In addition, the PDC and NEP demonstrated statistically significant correlations with interstitial inflammation (p = 0.018/p = 0.028). Regarding electron microscopy evaluation, PDC levels were correlated with distinct characteristics, such as fibrils and global podocyte foot process fusion, whereas the NEP/CR ratio was uniquely significantly associated with podocyte fusion only in non-immune-complex-mediated glomerulonephritis (p = 0.02). Among the other clinical and histological parameters included in our study, a strong correlation between proteinuria >3 g/24 h and diffuse fusion of podocyte foot processes (p = 0.016) was identified. Conclusions: Podocalyxin and nephrin concentrations in urine are markers of podocyte dysfunction, and in our study, they were associated both with serum creatinine and histological chronicity indices.

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Nephrin and Podocalyxin - New Podocyte Proteins for Early Detection of Secondary Nephropathies

Cited by 8 publications

References 42 publications

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

The Effectiveness of N-acetylcysteine in Alleviating Kidney Dysfunction in Ifosfamide-treated Rats

Urine Nephrin and Podocalyxin Reflecting Podocyte Damage and Severity of Kidney Disease in Various Glomerular Diseases—A Cross-Sectional Study

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