Nepeta nuda ssp. nuda L. water extract: Inhibition of replication of some strains of human alpha herpes virus (genus simplex virus) in vitro, mode of action and NMR-based metabolomics

Hinkov, Anton; Angelova, Petia; Marchev, Andrey S.; Hodzhev, Yordan; Tsvetkov, V. V; Dragolova, D.; Todorov, Daniel; Shishkova, Kalina; Kapchina-Toteva, Veneta; Blundell, Renald; Shishkov, Stoyan; Georgiev, Milen I.

doi:10.1016/j.hermed.2020.100334

Cited by 12 publications

(8 citation statements)

References 41 publications

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“…argolica , contain two nepetalactones and 1,5,9-epideoxyloganic acid (1,5,9- e DLA) as major iridoids (Aničić et al, 2021 ). The composition and importance of N. nuda phenolic compounds have been also previously reported, giving a special emphasis to phenolic acids, their derivatives, and flavonoids (Mišić et al, 2015 ; Aras et al, 2016b ; Fraga et al, 2017 ; Dienaite et al, 2018 ; Smiljković et al, 2018 ; Sarikurkcu et al, 2019 ; Hinkov et al, 2020 ; Aničić et al, 2021 ).…”

Section: Introductionmentioning

confidence: 78%

“…The strain F of Human alphaherpesvirus type 1 (HHV-1) (purchased from ATCC) was propagated in MDBK cells and stored at −70°C (BINDER GmbH, Germany) until used. This HHV-1 strain is sensitive to the antiviral agent acyclovir (Hinkov et al, 2020 ). The virus titer was determined by cytopathic effect (CPE) assay using the method of Reed and Muench ( 1938 ) and plaque assay (Dulbecco, 1952 ) in MDBK.…”

Section: Methodsmentioning

confidence: 99%

“…Aqueous extracts exhibit allelopathic activity by inhibiting the growth of wheat and cucumber (Dragoeva et al, 2017 ). On the other hand, they possess antioxidant, anti-proliferative and antiviral activities (Dienaite et al, 2018 ; Hinkov et al, 2020 ). Nepeta nuda tincture is effective against oral pathogens (Smiljković et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

et al. 2022

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Nepeta nuda (catmint; Lamiaceae) is a perennial medicinal plant with a wide geographic distribution in Europe and Asia. This study first characterized the taxonomic position of N. nuda using DNA barcoding technology. Since medicinal plants are rich in secondary metabolites contributing to their adaptive immune response, we explored the N. nuda metabolic adjustment operating under variable environments. Through comparative analysis of wild-grown and in vitro cultivated plants, we assessed the change in phenolic and iridoid compounds, and the associated immune activities. The wild-grown plants from different Bulgarian locations contained variable amounts of phenolic compounds manifested by a general increase in flowers, as compared to leaves, while a strong reduction was observed in the in vitro plants. A similar trend was noted for the antioxidant and anti-herpesvirus activity of the extracts. The antimicrobial potential, however, was very similar, regardless the growth conditions. Analysis of the N. nuda extracts led to identification of 63 compounds including phenolic acids and derivatives, flavonoids, and iridoids. Quantification of the content of 21 target compounds indicated their general reduction in the extracts from in vitro plants, and only the ferulic acid (FA) was specifically increased. Cultivation of in vitro plants under different light quality and intensity indicated that these variable light conditions altered the content of bioactive compounds, such as aesculin, FA, rosmarinic acid, cirsimaritin, naringenin, rutin, isoquercetin, epideoxyloganic acid, chlorogenic acid. Thus, this study generated novel information on the regulation of N. nuda productivity using light and other cultivation conditions, which could be exploited for biotechnological purposes.

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Section: Introductionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

et al. 2022

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“…NMR has a stand-alone contribution in uncovering the genetic variants that cause changes in phenotype and metabolism ( Rueedi et al, 2017 ). NMR applications have been extended from plant ( Ali et al, 2020 , 2021 ; Dutra et al, 2020 ) to human diseases ( Tayanloo-Beik et al, 2020 ; Fuertes-Martín and VallvéAmigó, 2020 ), viral events ( Hinkov et al, 2020 ; Sharma and Varani, 2020 ; Jaiswal et al, 2020 ), COVID-19 phenotyping ( Kimhofer et al, 2020 ), immunometabolism and metabolic pathways depiction. Based on its potential, NMR has large-scale applications in SARS-CoV-2, thus Table 1 depicts a few examples of how NMR has been applied in COVID-19 pandemic.…”

Section: Nuclear Magnetic Resonance (Nmr) Spectroscopymentioning

confidence: 99%

NMR spectroscopy spotlighting immunogenicity induced by COVID-19 vaccination to mitigate future health concerns

Ali

Nedvědová

Badshah

et al. 2022

Current Research in Immunology

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“…In a recent study, water-based extracts of Nepeta cataria significantly inhibited the replication of the herpes virus in humans. Moreover, various phenolic compounds and flavonoids were detected by NMR technology [8]. A few other studies have been reported secondary metabolites [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

The Phytochemical analysis, Metabolic profiling, Anti-bacterial and Anti-oxidant activity of Nepeta cataria

Nadeem

Ahmad

Imran

et al. 2021

Preprint

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Introduction: Nepeta cataria is a naturally growing plant in the regions of Pakistan, in English name is Catnip mint and the local name is Badranj boya. This plant has immense pharmacological and medical importance. It is used in herbal and traditional medicine as an antipathogenic agent. However detailed phytochemical analyses in a wide spectrum of solvents and their antimicrobial analysis against both Gram-positive and negative limited studied. Objectives: This study aimed to identify phytochemicals, metabolome, antimicrobial potential, and antioxidant analysis of Nepeta cataria (Pakistani plant). Methods: Plant collection, identification, cleaning, and grinding were performed followed by extract preparation in five solvents i.e. methanol, ethanol, water, acetone, and hexane. Qualitative phytochemical analysis was performed by general biochemical tests. . GC/MS was used to identify metabolic compounds in all extracts. Antibacterial analysis for both gram-positive and negative bacteria was performed by Kirby’s Disc method, 96 well test, and Resazurin test. The antioxidant activity was determined by DPPH assay. Results: Kirby disk diffusion method results indicated that Nepeta cataria extracts in different solvents maximum inhibition percentage against tested bacteria was 250-1000 µg/ml. Resazurin method inhibition against bacterial strains was 6.25-100 µl/ml. The Phytochemical analysis for Phenolic Compounds, Flavonoids, Terpenoids, Cardiac Glycosides, Free Anthraquinones, Combined Anthraquinones, Tannins, Alkaloids, and DPPH was positive in the different extracts. The ORAC was 133759.021um/100ml in concentration. GC/MS analysis of methanolic extract (71 identified + 48 unknown),ethanol-based extract ( 80 known + 31unknown), water-based extract ( 28 known + 11 unknown ), acetone-based extract ( 13 known + 9 unknown) , hexane based extract (11 known + 8 unmatched) phytochemicals were detected. Conclusion: Current study concludes the remarkable antibacterial and antioxidant potential of Nepeta cataria extracts.

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Nepeta nuda ssp. nuda L. water extract: Inhibition of replication of some strains of human alpha herpes virus (genus simplex virus) in vitro, mode of action and NMR-based metabolomics

Cited by 12 publications

References 41 publications

Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

NMR spectroscopy spotlighting immunogenicity induced by COVID-19 vaccination to mitigate future health concerns

The Phytochemical analysis, Metabolic profiling, Anti-bacterial and Anti-oxidant activity of Nepeta cataria

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