Neospora caninum Antigens Recognized by Mouse IgG at Different Stages of Infection Including Recrudescence

Tomioka, Y.; Sawada, Masumi; Ochiai, Kenji; Umemura, Takashi

doi:10.1292/jvms.65.745

Cited by 1 publication

(3 citation statements)

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“…Tomioka et al . (27) stated that in an early stage of infection, a 36–38 kDa antigen was clearly recognized by mouse antisera, but the signal weakened after day 48 post‐inoculation.…”

Section: Discussionmentioning

confidence: 99%

“…Hemphill and Gottstein (24), Hemphill et al (25,26), and Sonda et al (15) reported that the 36 kDa antigen was a major tachyzoite surface protein. Tomioka et al (27) stated that in an early stage of infection, a 36-38 kDa antigen was clearly recognized by mouse antisera, but the signal weakened after day 48 post-inoculation.…”

Section: Discussionmentioning

confidence: 99%

“…Bjerkas et al . (10) showed that immune sera from a wide range of animal species exhibited a similar recognition pattern when visualized by immunoblotting, with five major (17,27,29,30 and 46 kDa) and several minor N. caninum antigenic bands. The 17-kDa antigen was found to be localized in the rhoptries, while the 29-and 30-kDa antigens were distributed in the parasitophorous vacuole network (10).…”

Section: Introductionmentioning

confidence: 96%

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Recognition patterns of Neospora caninum tachyzoite antigens by bovine IgG at different IFAT titres

Okeoma

Williamson

Pomroy

et al. 2004

Parasite Immunology

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This study describes qualitative and quantitative antibody response in cows naturally infected with Neospora caninum. The study was carried out with 269 serum samples obtained from 24 cows over a period of 15 weeks. Prior to sample collection, the cows were tested with ELISA. The 269 samples were screened with IFAT and categorized into seven IFAT titre groups (< 1 : 80, 1 : 80, 1 : 200, 1 : 600, 1 : 1000, 1 : 2000, > 1 : 2000). The samples were finally analysed by Western blotting. Seven immunodominant antigens (approximately 18-, approximately 25-, approximately 33-, approximately 35-36-, approximately 45-46-, approximately 47-, approximately 60-62 kDa) and five minor antigens (approximately 25, approximately 51, approximately 64, approximately 77, approximately 116 kDa) were recognized by cow sera. The recognition of approximately 46 kDa antigen by cow sera was common to samples with IFAT titre 1 : 80 and above. Another common antigen was the approximately 18 kDa antigen, which was recognized by samples with IFAT titre 1 : 200 and above. The most remarkable observation was the presence of the 45-46 kDa, the 77 kDa, and absence of the 18 kDa antigenic bands in samples with IFAT titre 1 : 80. This observation was consistent even in the face of fluctuating antibody titre where serum antibody titres from an animal exceeded then failed to reach 1 : 80. Antibody fluctuation was observed across all cows (pregnant and aborted) with no discernible fluctuation pattern. However, the fluctuation in antibody titre observed appeared to be most remarkable in initially ELISA-negative pregnant cows, and to a lesser extent in ELISA-positive pregnant cows, and ELISA-positive aborted cows. Although there was fluctuation in antibody titre, the banding patterns of N. caninum tachyzoite antigens by cows within the same IFAT titre group remained similar.

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“…Tomioka et al . (27) stated that in an early stage of infection, a 36–38 kDa antigen was clearly recognized by mouse antisera, but the signal weakened after day 48 post‐inoculation.…”

Section: Discussionmentioning

confidence: 99%