Neoplastic transformation by quartz in the BALB/3T3/A31-1-1 cell line and the effects of associated minerals

Saffiotti, Umberto; Ahmed, Nadera

doi:10.1002/(sici)1520-6866(1996)15:6<339::aid-tcm8>3.0.co;2-j

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…In the present work, we have shown that crystallized silica (Chd and Min-U-Sil) was able to induce transformed SHE colonies in a dose-dependent manner after seven days of treatment. Our results are in agreement with previous transformation studies on quartz [18], [21], [22], [23] and [24]. Hesterberg and Barrett [21] showed that two quartz samples induced a concentration-dependent increase in TF, and both of these samples were less potent than asbestos fibres.…”

Section: The She Cell Transformation Assay Which Uses Primary Cell Csupporting

confidence: 93%

In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells

Darné

Coulais

Terzetti

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Crystalline silica particles and asbestos have both been classified as carcinogenic by the International Agency for Research on Cancer (IARC). However, because of the limited data available, amorphous silica was not classifiable. In vitro, the carcinogenic potential of natural crystalline and amorphous silica particles has been revealed by the Syrian Hamster Embryo (SHE) cell transformation assay. On the other hand, the genotoxic potential of those substances has not been investigated in SHE cells. And yet, genotoxicity assays are commonly used for hazard evaluation and they are often used as in vitro assays of reference to predict a possible carcinogenic potential. The main objective of this study was to compare the genotoxic potential and the carcinogenic potential of different crystalline and amorphous silica particles in SHE cells. Three silica samples of different crystallinity were used: natural amorphous silica, partially crystallized silica and quartz silica particles. Their genotoxicity were tested through the in vitro micronucleus assay and the comet assay in SHE, and their carcinogenic potential through the SHE transformation assay. In addition, silica samples were also tested with the same genotoxicity assays in V79 hamster-lung cells, a common in vitro model for particle exposure. Results obtained in the micronucleus and the comet assays show that none of the silica was capable of inducing genotoxic effects in SHE cells and only the amorphous silica induced genotoxic effects in V79 cells. However in the SHE cell transformation assays, the partially crystallized and quartz silica were able to induce morphological cell transformation. Together, these data suggest that, in vitro, the short-term genotoxic assays alone are not sufficient to predict the hazard and the carcinogenic potential of this type of particles; SHE transformation assay appears a more reliable tool for this purpose and should be included in the "in vitro battery assays" for hazard assessment.

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Section: The She Cell Transformation Assay Which Uses Primary Cell Csupporting

confidence: 93%

In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells

Darné

Coulais

Terzetti

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Binding of the DNA phosphate backbone to the silanol groups in the quartz surface, close to the sites of oxygen radicals production, has been suggested as the mechanism for silica-induced neoplastic transformation [Saf®otti et al, 1996]. A reduction in silica toxicity and neoplastic transforming activity in cultured cells was observed when hematite or anatase, a polymorph of titanium dioxide (TiO 2 ), were combined with silica [Saf®otti and Ahmed, 1995]. This would indicate that crystal surfaces of hematite and anatase, non-toxic by themselves, may block critical steps of quartz cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Lung cancer risk, silica exposure, and silicosis in Chinese mines and pottery factories: The modifying role of other workplace lung carcinogens*

Cocco

Rice

Chen

et al. 2001

American J Industrial Med

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Our results suggest that, among silica-exposed Chinese workers, numerous occupational and non-occupational risk factors interact in a complex fashion to modify lung cancer risk. Future epidemiological studies on silica and lung cancer should incorporate detailed information on exposure to other workplace lung carcinogens, total respirable dust, and on surface size and age of silica particles to understand whether and to what extent they affect the carcinogenic potential of silica.

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“…In addition, silica particles can induce oxidative stress leading to DNA damage and are responsible for gene silencing through DNA methylation of genes involved in cell cycle regulation (Belinsky et al, 2002, Deshpande et al, 2002, Knaapen et al, 2002. While treatment by the crystalline silica microparticle Min-U-Sil® 5 induced in vitro neoplastic transformation, treatment by amorphous silica nanoparticles did not induce BALB/3T3 or SHE cell transformation (Darne et al, 2016, Saffiotti and Ahmed, 1995, Uboldi et al, 2012. However, recent studies described that synthetic amorphous silica nanoparticles induce in in vitro models oxidative stress and genotoxic effects or in in vivo models cellular cell death and fibrosis , Murugadoss et al, 2017.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic changes in the early stage of silica-induced cell transformation

et al. 2017

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The increasing use of nanomaterials in numerous domains has led to growing concern about their potential toxicological properties, and the potential risk to human health posed by silica nanoparticles remains under debate. Recent studies proposed that these particles could alter gene expression through the modulation of epigenetic marks, and the possible relationship between particle exposure and these mechanisms could represent a critical factor in carcinogenicity. In this study, using the Bhas 42 cell model, we compare the effects of exposure to two transforming particles, a pyrogenic amorphous silica nanoparticle NM-203 to those of the crystalline silica particle Min-U-Sil 5. Short-term treatment by Min-U-Sil 5 decreased global DNA methylation and increased the expression of the two de novo DNMTs, DNMT3a and DNMT3b. NM-203 treatment affected neither the expression of these enzymes nor DNA methylation. Moreover, modified global histone H4 acetylation status and HDAC protein levels were observed only in the Min-U-Sil 5-treated cells. Finally, both types of particle treatment induced strong c-Myc expression in the early stage of cell transformation and this correlated with enrichment in RNA polymerase II as well as histone active marks on its promoter. Lastly, almost all parameters that were modulated in the early stage were restored in transformed cells suggesting their involvement mainly in the first steps of cell transformation.

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Neoplastic transformation by quartz in the BALB/3T3/A31-1-1 cell line and the effects of associated minerals

Cited by 13 publications

References 19 publications

In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells

In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells

Lung cancer risk, silica exposure, and silicosis in Chinese mines and pottery factories: The modifying role of other workplace lung carcinogens*

Epigenetic changes in the early stage of silica-induced cell transformation

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