Neoplastic transformation and induction of H+,K+-adenosine triphosphatase by N-methyl-N′-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line

Shimokawa, Osamu; Matsui, Hirofumi; Nagano, Yumiko; Kaneko, Tsuyoshi; Shibahara, Takeshi; Nakahara, Akira; Hyodo, Ichinosuke; Yanaka, Akinori; Majima, Hideyuki J.; Nakamura, Yukio; Matsuzaki, Yasushi

doi:10.1007/s11626-007-9067-8

Cited by 43 publications

(45 citation statements)

References 21 publications

(15 reference statements)

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“…RGK35 and RGM36 were established in our previous studies. RGK and RGM were cultured in Dulbecco's modified eagle's medium nutrient mixture F-12 HAM (Life technologies, Carlsbad, CA) and DMEM/F12 (Life technologies), respectively, in a 5% CO2 humidified atmosphere at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques

Tamura

Yanagawa

Sugiura

et al. 2014

Sci Rep

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Cell sorting is an essential and efficient experimental tool for the isolation and characterization of target cells. A three-dimensional environment is crucial in determining cell behavior and cell fate in biological analysis. Herein, we have applied photodegradable hydrogels to optical cell separation from a 3D environment using a computer-controlled light irradiation system. The hydrogel is composed of photocleavable tetra-arm polyethylene glycol and gelatin, which optimized cytocompatibility to adjust a composition of crosslinker and gelatin. Local light irradiation could degrade the hydrogel corresponding to the micropattern image designed on a laptop; minimum resolution of photodegradation was estimated at 20 µm. Light irradiation separated an encapsulated fluorescent microbead without any contamination of neighbor beads, even at multiple targets. Upon selective separation of target cells in the hydrogels, the separated cells have grown on another dish, resulting in pure culture. Cell encapsulation, light irradiation and degradation products exhibited negligible cytotoxicity in overall process.

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Section: Methodsmentioning

confidence: 99%

Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques

Tamura

Yanagawa

Sugiura

et al. 2014

Sci Rep

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“…The rat gastric mucosal cell line RGM-1 (RCB-0876 at the Riken Cell Bank, Tsukuba, Japan), established by Matsui and Ohno, was used (Kobayashi et al, 1996). RGM-1 cells have the characteristics of gastric mucous-producing cells, and they have prostaglandin EP 4 receptors (Kobayashi et al, 1996) and do not express parietal cell-specific H ϩ , K ϩ -ATPase (Shimokawa et al, 2007). RGM-1 cells were grown in a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM glutamine, 100 U/ml penicillin, 100 g/ml streptomycin, and 0.25 g/ml amphotericin.…”

Section: Methodsmentioning

confidence: 99%

Lansoprazole, a Proton Pump Inhibitor, Mediates Anti-Inflammatory Effect in Gastric Mucosal Cells through the Induction of Heme Oxygenase-1 via Activation of NF-E2-Related Factor 2 and Oxidation of Kelch-Like ECH-Associating Protein 1

Takagi

Naito

Okada

et al. 2009

J Pharmacol Exp Ther

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Induction of heme oxygenase-1 (HO-1) expression has been associated with cytoprotective and anti-inflammatory actions of lansoprazole, a proton pump inhibitor, but the underlying molecular mechanisms remain largely unresolved. In this study, we investigate the role of transcriptional NF-E2-related factor 2 (Nrf2), its phosphorylation/activation, and oxidation of Kelch-like ECHassociating protein 1 (Keap1) in lansoprazole-induced HO-1 up-regulation using cultured gastric epithelial cells (rat gastric mucosal cell line, RGM-1). HO-1 expression of RGM-1 cells was markedly enhanced in a time-and dose-dependent manner by the treatment with lansoprazole, and this up-regulation of HO-1 contributed to the inhibition of chemokine production from stimulated RGM-1 cells. Transfection of Nrf2-siRNA suppressed the lansoprazole-induced HO-1. An electrophoretic mobility shift assay showed increases in the nuclear translocation and stress-response elements (StRE) binding activity of Nrf2 proteins in RGM-1 cells treated with lansoprazole. Furthermore, in RGM-1 cells transfected with HO-1 enhancer luciferase reporter plasmid containing mutant StRE, lansoprazole-induced HO-1 reporter gene activity was diminished. Lansoprazole promoted the phosphorylation of extracellular signal-regulated kinase (ERK), and lansoprazole-induced HO-1 up-regulation was suppressed by U0126, an ERKspecific inhibitor. Phosphorylated Nrf2 protein was detected in the phosphoprotein fraction purified by a Pro-Q Diamond Phosphoprotein Enrichment kit. Finally, an oxidative form of the Keap1 protein was detected in lansoprazole-treated RGM-1 cells by analyzing S-oxidized proteins using biotinylated cysteine as a molecular probe. These results indicate that lansoprazole up-regulates HO-1 expression in rat gastric epithelial cells, and the upregulated HO-1 contributes to the anti-inflammatory effects of the drug. Phosphorylation of ERK and Nrf2, activation and nuclear translocation of Nrf2, and oxidation of Keap1 are all involved in the lansoprazole-induced HO-1 up-regulation.Proton pump inhibitors (PPIs) such as lansoprazole and omeprazole are extensively used to treat acid-related disorders, including gastroesophageal reflux disease and peptic ulcer disease caused by stress, nonsteroidal anti-inflammatory drugs, and Helicobacter pylori infection. PPIs are strong

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“…We have investigated the relation between mitROS and cancer cellular invasion using rat gastric mucosal cells, (8) RGM1, (9) its cancer-like mutant cells induced by oncogenic chemicals, RGK1 (10) and manganese superoxide dismutase (MnSOD)-expressed RGK cells, RGK-MnSOD, which we have established previously. (8) MnSOD is a nuclear-encoded primary antioxidant enzyme which has an ability to scavenge superoxide radicals in mitochondria selectively.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial reactive oxygen species accelerate the expression of heme carrier protein 1 and enhance photodynamic cancer therapy effect

Ito

Matsui

Tamura

et al. 2014

J. Clin. Biochem. Nutr.

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Photodynamic therapy using hematoporphyrin and its derivatives is clinically useful for cancer treatments. It has been reported that cancer cells incorporate hematoporphyrin and its derivatives via heme carrier protein 1, which is a proton-coupled folate transporter. However, the mechanism of this protein expression has not been elucidated. In general, the concentration of reactive oxygen species in cancer cells is higher than that in normal cells. We previously reported that reactive oxygen species from mitochondria involved in the expression of peptide transporter 1 and accelerate the uptake of 5-aminolevulinic acid, which is a precursor of protoporphyrin IX. We suggested mitochondrial reactive oxygen species also regulated the expression of heme carrier protein 1. In this study, we used a rat gastric mucosal cell line RGM1 and its cancer-like mutated cell line RGK1. We clarified the expression of heme carrier protein 1 increased in cancer cells and it decreased in manganese superoxide dismutase expressed cancer cells. In addition, the uptake level of hematoporphyrin and photodynamic therapeutic effect were also decreased in manganese superoxide dismutase expressed cancer cells in comparison with cancer cells. Thus, we concluded that mitochondrial reactive oxygen species regulated heme carrier protein 1 expression and photodynamic therapeutic effect.

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Neoplastic transformation and induction of H+,K+-adenosine triphosphatase by N-methyl-N′-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line

Cited by 43 publications

References 21 publications

Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques

Optical cell separation from three-dimensional environment in photodegradable hydrogels for pure culture techniques

Lansoprazole, a Proton Pump Inhibitor, Mediates Anti-Inflammatory Effect in Gastric Mucosal Cells through the Induction of Heme Oxygenase-1 via Activation of NF-E2-Related Factor 2 and Oxidation of Kelch-Like ECH-Associating Protein 1

Mitochondrial reactive oxygen species accelerate the expression of heme carrier protein 1 and enhance photodynamic cancer therapy effect

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