Neoplasia-related changes of two copper (Cu)/zinc (Zn) proteins in the human colon

Mulder, T.P.J.; Verspaget, Hein W.; Janssens, A. R.; Bruin, P.A.F. de; Griffioen, G.; Lamers, C. B. H. W.

doi:10.1016/0891-5849(90)90128-6

Cited by 28 publications

(25 citation statements)

References 23 publications

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“…36,37 This was reported as well by Frederiks and Bosch. 26 Mulder et al 38 found a significant increase in CuZn-SOD levels in homogenates in the sequence of normal mucosa-adenoma-carcinoma. A decrease in CuZn-SOD expression was observed in only 18% of the colorectal carcinomas, whereas we found decreased expression in 83% of the cases.…”

Section: Discussionmentioning

confidence: 98%

Expression of CuZn- and Mn-Superoxide Dismutase in Human Colorectal Neoplasms

Driel

Lyon

Hoogenraad

et al. 1997

Free Radical Biology and Medicine

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Abstract-Decreased intracellular SOD protein levels and activity have been related with malignancy in the past. To investigate their relevance in the carcinogenetic process in the colon, we determined quantitatively CuZn-SOD and Mn-SOD levels and total SOD activity by histochemical means in human normal colorectal mucosa, adenomas, and carcinomas. Protein levels and activity were significantly decreased in carcinomas. CuZn-SOD protein levels, but not Mn-SOD levels or total SOD activity were related with differentiation grade and to a lesser extent with Dukes stage. Moderately differentiated carcinomas and Dukes stage A carcinomas showed lowest levels. Some carcinomas expressed elevated levels of CuZn-SOD and this was an indication of poor survival. It is concluded that decreased SOD expression is not a prognostic marker and seems to be a secondary phenomenon rather than directly linked with the carcinogenetic process. ᭧ 1997 Elsevier Science Inc.

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Section: Discussionmentioning

confidence: 98%

Expression of CuZn- and Mn-Superoxide Dismutase in Human Colorectal Neoplasms

Driel

Lyon

Hoogenraad

et al. 1997

Free Radical Biology and Medicine

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“…The samples were wet-weighed, and 1 ml of 0.1 M TRIS-HCl (pH 7.5) supplemented with 0.1% (v/v) Tween-80 extraction buer per 60 mg of sample was added as described previously (Mulder et al 1990). This buer was found not to interfere in the subsequent SOD assessments.…”

Section: Tissue Preparation and Protein Concentrationmentioning

confidence: 99%

Superoxide dismutases in the human colorectal cancer sequence

Janssen¹,

Bosman²,

Kruidenier³

et al. 1999

Journal of Cancer Research and Clinical Oncology

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“…MT expression in colorectal cancer (CRC) has been studied by radioimmunoassay and immunohistochemistry (IHC) using antibodies that recognize isoforms 1 and 2 and shown to be progressively down-regulated during CRC progression [6,7]. Although the prognostic significance of MT expression in CRC has not been clearly defined, several studies agree that tumors with lower MT expression have worse prognosis [8].…”

Section: Introductionmentioning

confidence: 99%