Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana

Bai, Ya-Li; Yin, Xiao‐Ming; Xiong, Cai-Feng; Cai, Bingna; Wu, Yan; Zhang, Xiaoyun; Wei, Zhenwei; Ye, Tiantian; Feng, Yun

doi:10.1016/j.xplc.2022.100340

Cited by 5 publications

(17 citation statements)

References 42 publications

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“…As shown in Figure C, low-abundance [M + H – H 2 O] + fragment ion ( m/z 335.2330) was observed in the MS 2 spectrum of Compound 10, suggesting that Compound 10 was more likely to be in ABA 9′-hydroxylation pathway (rule 3), and thus Compound 10 was more likely to be neoDPA or epi -neoDPA. In our recent published work, Compound 10 has been characterized as epi -neoDPA by comparing its mass, retention time, and MS n spectra with those of our chemically synthesized epi -neoDPA . The possible fragmentation pathways of DMED-labeled Compound 10 are shown in Figure S43.…”

Section: Resultsmentioning

confidence: 99%

“…In our recent work, we speculated that neoPA could be further reduced into compound (21) or (22), named as neodihydrophaseic acid (neoDPA) and epi-neodihydrophaseic acid (epi-neoDPA), respectively (Figure 1B). 13 Catabolites such as 9′-oxo-ABA and 9′-OH-neoPA might also exist in the ABA 9′-hydroxylation pathway, which were corresponding to 8′-oxo-ABA and 8′-OH-PA reported in the ABA 8′hydroxylation pathway. Our predicted structures are summarized in Figure 1B.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In our recent published work, Compound 10 has been characterized as epi-neoDPA by comparing its mass, retention time, and MS n spectra with those of our chemically synthesized epi-neoDPA. 13 The possible fragmentation pathways of DMED-labeled Compound 10 are shown in Figure S43.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Some studies have shown that ABA catabolites also play an important role in plant growth and development. For instance, 7′-OH ABA and neoPA have been reported to inhibit seed germination. , Furthermore, it has been found that PA with ABA-like hormonal activity can selectively activate a subset of the ABA receptor family members and enhance oil synthesis in Brassica seeds. − Accordingly, it is essential to investigate ABA catabolism for the improvement of the ABA catabolism network.…”

Section: Introductionmentioning

confidence: 99%

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Screening and Identification of Potential Abscisic Acid Catabolites by Chemical Labeling-Assisted Ultrahigh-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Bai

Xiong

Yin

et al. 2022

J. Agric. Food Chem.

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In this study, a screening strategy was established based on ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry assisted by chemical isotope labeling (CIL-UPLC-HRMS) for screening and identifying abscisic acid (ABA) catabolites. Based on the structures of known ABA catabolites, this strategy first proposed the structures of catabolites to be discovered. Afterward, a pair of isotope reagents N,N-2-dimethylaminoethylamine (DMED) and d 4 -DMED were used as labeling reagents to label the carboxyl groups in ABA and its catabolites. Then, the mass-to-charge ratio (m/z) of DMEDand d 4 -DMED-labeled ABA catabolites was calculated based on the labeling schema. In light of the characteristic fragmentation patterns of the DMED-labeled standards of ABA and its catabolites, screening criteria were formulated. Using our strategy, ABA, t-ABA, and 18 ABA catabolites were identified from seven plant samples. Of the identified catabolites, 16 were known, and to our knowledge, 2 were previously unidentified. Our findings contribute to ABA catabolic network improvement.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Screening and Identification of Potential Abscisic Acid Catabolites by Chemical Labeling-Assisted Ultrahigh-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Bai

Xiong

Yin

et al. 2022

J. Agric. Food Chem.

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“…Further, 7OH-ABA demonstrated an accumulation pattern similar to ABA ( r = 0.89), with even more marked accumulation under progressive water stress and a higher total amount compared to ABA. This finding raises the question of the biological importance of 7OH-ABA accumulation; 7OH-ABA is considered a minor product of ABA hydroxylation [ 28 ], but, in conifers, it can be present in substantial amounts [ 29 ]. Neither the enzyme responsible for ABA C7′-hydroxylation nor further metabolism of 7OH-ABA are currently known [ 28 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Abscisic Acid and Cytokinins Are Not Involved in the Regulation of Stomatal Conductance of Scots Pine Saplings during Post-Drought Recovery

et al. 2023

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Delayed or incomplete recovery of gas exchange after water stress relief limits assimilation in the post-drought period and can thus negatively affect the processes of post-drought recovery. Abscisic acid (ABA) accumulation and antagonistic action between ABA and cytokinins (CKs) play an important role in regulation of stomatal conductance under water deficit. Specifically, in pine species, sustained ABA accumulation is thought to be the main cause of delayed post-drought gas exchange recovery, although the role of CKs is not yet known. Therefore, we aimed to study the effects of ABA and CKs on recovery of stomatal conductance in greenhouse-grown 3-year-old Scots pine saplings recovering from water stress. We analysed both changes in endogenous ABA and CK contents and the effects of treatment with exogenous CK on stomatal conductance. Drought stress suppressed stomatal conductance, and post-drought stomatal conductance remained suppressed for 2 weeks after plant rewatering. ABA accumulated during water stress, but ABA levels decreased rapidly after rewatering. Additionally, trans-zeatin/ABA and isopentenyladenine/ABA ratios, which were decreased in water-stressed plants, recovered rapidly in rewatered plants. Spraying plants with 6-benzylaminopurine (0.1–100 µM) did not influence recovery of either stomatal conductance or needle water status. It can be concluded that the delayed recovery of stomatal conductance in Scots pine needles was not due to sustained ABA accumulation or a sustained decrease in the CK/ABA ratio, and CK supplementation was unable to overcome this delayed recovery.

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A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry

Turečková,

Oklestková,

Žukauskaitė

et al. 2024

J Plant Growth Regul

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Abscisic acid (ABA) is a phytohormone that occurs in plants at very low concentration (pmol/g fresh weight) and regulates multiple biological processes, including stomatal closure, seed germination, and responses to environmental stresses. In the present study, isolation of ABA, ABA glucosyl ester, and 11 ABA amino acid conjugates from minute quantities of plant tissue (less than 20 mg fresh weight) was achieved using a purification method based on the combination of an Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate, was isolated by IAC and identified by tandem mass spectrometry in pea (Pisum sativum L.), Lepidium sativum L. and wheat (Triticum aestivum L.) seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana or water-stressed tobacco (Nicotiana tabacum L.) leaves. Here, the identification of an ABA conjugate with glutamic acid in plants is described for the first time.

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Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana

Cited by 5 publications

References 42 publications

Screening and Identification of Potential Abscisic Acid Catabolites by Chemical Labeling-Assisted Ultrahigh-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Screening and Identification of Potential Abscisic Acid Catabolites by Chemical Labeling-Assisted Ultrahigh-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Abscisic Acid and Cytokinins Are Not Involved in the Regulation of Stomatal Conductance of Scots Pine Saplings during Post-Drought Recovery

A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry

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