Neonatal treatment with monosodium glutamate lastingly facilitates spreading depression in the rat cortex

Lima, Cássia Borges; Soares, Geórgia de Sousa Ferreira; Vitor, Suênia Marcele; Castellano, Bernardo; Costa, Belmira da Silveira Andrade da Lara; Guedes, Rubem Carlos Araújo

doi:10.1016/j.lfs.2013.07.009

Cited by 26 publications

(14 citation statements)

References 45 publications

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“…The following results are observed in Fig. 3: exercising decelerates CSD compared to the corresponding sedentary groups; the previous data regarding the MSG effect on CSD propagation in sedentary rats is confirmed (Lima et al, 2013); the exercised group treated with 2 g/kg MSG tended to present with higher CSD propagation velocities compared to the corresponding sedentary group, but this tendency was not significant ( P < 0.061).…”

Section: Resultsmentioning

confidence: 55%

“…Fig. 3C compares the MSG‐treated and exercised groups from the present study with the MSG‐treated and sedentary animals in our previous study (Lima et al, 2013). ANOVA revealed a non‐significant trend ( P < 0.061) of interaction between both factors (MSG versus exercise).…”

Section: Resultsmentioning

confidence: 70%

“…Sal, saline; Nv, naïve, no treatment; MSG‐2, 2 g/kg MSG; MSG‐4, 4 g/kg MSG (subcutaneous injections). (C) Comparison between the MSG‐treated and exercised groups from the present study with the MSG‐treated and sedentary animals in our previous study (Lima et al, 2013). Data are expressed as mean ± S.E.M.…”

Section: Resultsmentioning

confidence: 82%

“…We previously reported that MSG treatment dose‐dependently increases microglial Iba1 immunolabeling in the motor cortex of sedentary rats (Lima et al, 2013). In the present study, the exercise in MSG treated animals decreased Iba1 immunoreactivity intensity and increased the immunolabeled area.…”

Section: Discussionmentioning

confidence: 99%

“…Male Wistar rat pups ( n = 28) were treated with MSG as previously described (Lima et al, 2013). Briefly, the animals received subcutaneous injections of 2 g/kg ( n = 13; MSG‐2) or 4 g/kg MSG ( n = 15; MSG‐4) administered every other day during the first 14 days of life.…”

Section: Methodsmentioning

confidence: 99%

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Spreading depression features and Iba1 immunoreactivity in the cerebral cortex of developing rats submitted to treadmill exercise after treatment with monosodium glutamate

Lima

Soares

Vitor

et al. 2013

Intl J of Devlp Neuroscience

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Physical exercise and excessive consumption of monosodium glutamate (MSG) can affect the morphological and electrophysiological organization of the brain during development. However, the interaction of both factors remains unclear. We analyzed the effect of this interaction on the excitability-related phenomenon known as cortical spreading depression (CSD) and the microglial reaction expressed as Iba1-immunolabeled cells in the rat motor cortex. MSG (2g/kg or 4g/kg) was administered every other day during the first 14 postnatal days. Treadmill exercise started at 21-23 days of life and lasted 3 weeks, 5 days/week, for 30min/day. At 45-60 days, CSD was recorded for 4h at two cortical points and the CSD parameters (velocity, amplitude, and duration of the negative potential change) calculated. Confirming previous observations, exercised rats presented with lower CSD velocities (3.29±0.18mm/min) than the sedentary group (3.80±0.18mm/min; P<0.05). MSG increased CSD velocities in the exercised rats compared to saline-treated and exercised animals in a dose-dependent manner (3.49±0.19, 4.05±0.18, and 3.27±0.26 for 2g/kg MSG, 4g/kg MSG, and saline, respectively; P<0.05). The amplitude (ranging from 14.3±5.9 to 18.7±6.2mV) and duration (46.7±11.1 to 60.5±11.6s) of the negative slow potential shift of the CSD were similar in all groups. Both exercise and MSG treatment increased Iba1 immunolabeling. The results confirm that physical exercise decelerates CSD propagation. However, it does not impede the CSD-accelerating action of MSG. These effects were accompanied by a cortical microglia reaction. Therefore, the data suggest that treadmill exercise early in life can influence the development of cortical electrical activity.

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Section: Resultsmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Spreading depression features and Iba1 immunoreactivity in the cerebral cortex of developing rats submitted to treadmill exercise after treatment with monosodium glutamate

Lima

Soares

Vitor

et al. 2013

Intl J of Devlp Neuroscience

Self Cite

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Neonatal l-glutamine modulates anxiety-like behavior, cortical spreading depression, and microglial immunoreactivity: analysis in developing rats suckled on normal size- and large size litters

et al. 2016

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In mammals, L-glutamine (Gln) can alter the glutamate-Gln cycle and consequently brain excitability. Here, we investigated in developing rats the effect of treatment with different doses of Gln on anxiety-like behavior, cortical spreading depression (CSD), and microglial activation expressed as Iba1-immunoreactivity. Wistar rats were suckled in litters with 9 and 15 pups (groups L and L; respectively, normal size- and large size litters). From postnatal days (P) 7-27, the animals received Gln per gavage (250, 500 or 750 mg/kg/day), or vehicle (water), or no treatment (naive). At P28 and P30, we tested the animals, respectively, in the elevated plus maze and open field. At P30-35, we measured CSD parameters (velocity of propagation, amplitude, and duration). Fixative-perfused brains were processed for microglial immunolabeling with anti-IBA-1 antibodies to analyze cortical microglia. Rats treated with Gln presented an anxiolytic behavior and accelerated CSD propagation when compared to the water- and naive control groups. Furthermore, CSD velocity was higher (p< 0.001) in the L compared to the L condition. Gln treatment increased Iba1 immunolabeling both in the parietal cortex and CA1 hippocampus, indicating microglial activation. The Gln effect was dose-dependent for anxiety-like behavior and CSD in both litter sizes, and for microglial activation in the L groups. Besides confirming previous electrophysiological findings (CSD acceleration after Gln), our data demonstrate for the first time a behavioral and microglial activation that is associated with early Gln treatment in developing animals, and that is possibly operated via changes in brain excitability.

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Toxicological evaluation of MSG for the manufacturing workers’ health: A literature review

Rim

2017

Toxicol. Environ. Health Sci.

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Neonatal treatment with monosodium glutamate lastingly facilitates spreading depression in the rat cortex

Cited by 26 publications

References 45 publications

Spreading depression features and Iba1 immunoreactivity in the cerebral cortex of developing rats submitted to treadmill exercise after treatment with monosodium glutamate

Spreading depression features and Iba1 immunoreactivity in the cerebral cortex of developing rats submitted to treadmill exercise after treatment with monosodium glutamate

Neonatal l-glutamine modulates anxiety-like behavior, cortical spreading depression, and microglial immunoreactivity: analysis in developing rats suckled on normal size- and large size litters

Toxicological evaluation of MSG for the manufacturing workers’ health: A literature review

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