Neonatal shaking brain injury changes psychological stress-induced neuronal activity in adult male rats

Ueda, Seinosuke; Yamaguchi, Takashi; Ehara, Ayuka

doi:10.1016/j.neulet.2020.134744

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…In recent years, the methods used to construct animal models of psychological stress include electric foot stimulation, thermal stimulation, compulsive swimming treatment, restraint method, and so on, the stress model obtained by the restraint method can cause a typical nonspecific stress response in animals and does not cause direct trauma to the animal body [ 9 , 10 ]. In this research, stress animal models were obtained by a restraint method.…”

Section: Discussionmentioning

confidence: 99%

Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats

Han

2022

Computational Intelligence and Neuroscience

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To explore the changes of pain sensitivity (PS) in the masseter area (MA) in the rat model of psychological stress and the mechanism of action between spinal nucleus neurons and astrocytes in the trigeminal ganglion. The 40 Sprague-Dawley rats were randomly divided into control group (no treatment), group A (restraint stress (RS) 1 d), group B (RS 7 d), and group C (RS 14 d). The body weight growth rates (WGR) of rats in each group were compared and the difference of CORT and ACTH in serum was analyzed by ELISA. The open field test and the elevated “cross” maze test were adopted to detect the behavioral changes of rats. Finally, pain threshold of the MA in rats, the activation amount of brain tissue medulla oblongata parts astrocytes markers Glial fibrillary acidic protein (GFAP), and the protein expression of IL-1β and IL-1RI were detected. The results showed the WGR at 7 d and 14 d was greatly lower than control group ( P < 0.01 ). In addition, the activity level and serum CORT and ACTH levels AND mean pain threshold in the MA of groups B and C were greatly lower than control group ( P < 0.05 ). The activation rate of GFRP in group C ( P < 0.01 ) and the protein expression of IL-1β and IL-1RI ( P < 0.05 ) in rat trigeminal ganglion astrocytes of groups B and C was greatly higher than control group, indicating the increase of RS time, the release of IL-1β and IL-1RI can activate neurons and astrocytes in spinal trigeminal nucleus (STN) nerve and increase the PS of the MA.

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Section: Discussionmentioning

confidence: 99%

Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats

Han

2022

Computational Intelligence and Neuroscience

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“…Local oxidative stress was thus considered to have occurred in the present experimental model. We recently demonstrated that neonatal SBI causes persistent changes to brain activity in adults in specific regions that react to psychological stress exposure, and these changes lead to neuroendocrine and behavioral alterations [ 25 ]. Oxidative stress due to iron leakage from MHs is thus considered a primary cause of the pathophysiology in this model.…”

Section: Discussionmentioning

confidence: 99%

Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry

Taguchi

Ehara

Seo

et al. 2020

Acta Histochem. Cytochem

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Previous studies have shown that neonatal shaking brain injury (SBI) causes transient microhemorrhages (MHs) in the gray matter of the cerebral cortex and hippocampus. Iron deposits and iron-uptake cells are observed surrounding MHs in this SBI model, suggesting local hypoxic-ischemic conditions. However, whether the shaken pups suffered systemic hypoxic-ischemic conditions has remained uncertain. Further, histopathological correlations of MHs on magnetic resonance imaging (MRI) are still unclear. The present study examined MHs after neonatal SBI using a combination of histochemical and susceptibility-weighted imaging (SWI) analyses. Systemic oxygen saturation analyses indicated no significant difference between shaken and non-shaken pups. MHs on postnatal day 4 (P4) pups showed decreased signal intensity on SWI. Iron histochemistry revealed that these hypointense areas almost completely comprised red blood cells (RBCs). MHs that appeared on P4 gradually disappeared by P7–12 on SWI. These resolved areas contained small numbers of RBCs, numerous iron-positive cells, and punctate regions with iron reaction products. Perivascular iron products were evident after P12. These changes progressed faster in the hippocampus than in cortical areas. These changes in MHs following neonatal SBI may provide new insights into microvascular pathologies and impacts on brain functions as adults.

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Neonatal shaking brain injury changes psychological stress-induced neuronal activity in adult male rats

Cited by 2 publications

References 36 publications

Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats

Effect of Restraint Stress on Pain Sensitivity, Spinal Trigeminal Nucleus Neurons, and Astrocytes in the Masseter Area of Rats

Microhemorrhage in a Rat Model of Neonatal Shaking Brain Injury: Correlation between MRI and Iron Histochemistry

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