Neonatal Rat Surgery: Avoiding Maternal Cannibalism

Libbin, Richard M.; Person, Philip

doi:10.1126/science.482926

Cited by 31 publications

(11 citation statements)

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“…They were handled daily to prevent cannibalism 23 . Delivery times were monitored and were accurate to within 12 h. The day of birth was considered day zero.…”

Section: Animalsmentioning

confidence: 99%

Corticotropin-releasing hormone is a rapid and potent convulsant in the infant rat

Baram

Schultz

1991

Developmental Brain Research

154

159

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Corticotropin-releasing hormone (CRH) administered into the cerebral ventricles of rats during the first postnatal week caused a specific and stereotyped behavior sequence: rhythmic chewing and licking (jaw myoclonus) were followed by 'litnbic'-type seizures. The onset of the seizures was much more rapid (2-45 min vs 3-7 h) than in adult rats, and the convulsant doses were much lower (50 × 10 −12 mol per gram brain weight vs 750 × 10 −12 mol per gram brain weight in adults). CRH potency in inducing seizures varied inversely with age. CRH-induced seizures occurred prior to any changes in serum corticosterone, and were eliminated by the administration of a CRH antagonist, as well as of phenytoin. Electrocorticographic correlates of CRH-induced behaviors in the infant rat were inconsistent, suggesting a subcortical origin of CRH-induced paroxysmal events in the immature brain.

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“…They were handled daily to prevent cannibalism 23 . Delivery times were monitored and were accurate to within 12 h. The day of birth was considered day zero.…”

Section: Animalsmentioning

confidence: 99%

Corticotropin-releasing hormone is a rapid and potent convulsant in the infant rat

Baram

Schultz

1991

Developmental Brain Research

154

159

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“…Rats were housed under a 12-h light/dark cycle, and fed ad libitum. They were handled daily to prevent cannibalism 14 . Delivery times were monitored and were accurate to within 12 h. The day of birth was considered day zero.…”

Section: Animalsmentioning

confidence: 99%

Bicuculline induced seizures in infant rats: ontogeny of behavioral and electrocortical phenomena

Baram

Snead

1990

Developmental Brain Research

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The effects of bicuculline, a γ-aminobutyric acid (GABA) antagonist, were investigated in 258 immature rats between the third and 22nd postnatal days. Behavioral and electrocorticographic events were correlated. Bicuculline induced both behavioral and electrographic seizures as early as the third postnatal day, an age when the CD 50 for bicuculline was lowest, and therefore the sensitivity to it was the greatest. Bicuculline may thus be a suitable convulsant for epilepsy studies involving rats during the first postnatal week.

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“…55th and 85th postnatal day. Neonatal castration was made by subumbilical incision, using the program described by Libbin and Person (1979] to abolish maternal cannibalism. Brains were fixed by perfusion in the left ventricle with Bouin's fluid and postfixed during 24 h in the same fluid, dehydrated and embedded in paraffin under standard conditions, to prevent that the shrinkage of the tissue interferes with the comparison of the different age groups and/or the comparison between the control and experimental groups.…”

Section: Methodsmentioning

confidence: 99%

Development of the Subfornical Organ and Area postrema of the Male Albino Mouse.Karyometric Effect of Neonatal and Prepuberal Castration

Castañeyra-Perdomo

Pérez-Delgado²,

González‐Hernández³

et al. 1988

Cells Tissues Organs

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We have studied the karyometric development of the nuclei of the ependymal cells and neurons of the subfornical organ and the area postrema in the male albino mouse from the 5th to the 190th postnatal day. We have found similar patterns of development in both although the area postrema showed more significant postnatal oscillations than those of the subfornical organ, suggesting a more intimate chronological relationship to gonadal development. We have furthermore analyzed the development in two experimental groups: in the one animals were castrated at birth, in the other, castration was made on the 20th postnatal day. We have found that neonatal castration produced a significant decrease of nuclear sizes; this was more evident in the subfornical organ than in the area postrema in earlier stages of development while the response was similar in both at peripuberal ages. The response to prepuberal castration was similar in both organs.

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Neonatal Rat Surgery: Avoiding Maternal Cannibalism

Abstract: A simple program of handling and care of pregnant rats before delivery makes it possible to carry out surgical procedures on newborn pups without resultant cannibalism or rejection of the operated animals by their mothers.

Cited by 31 publications

References 0 publications

Corticotropin-releasing hormone is a rapid and potent convulsant in the infant rat

Corticotropin-releasing hormone is a rapid and potent convulsant in the infant rat

Bicuculline induced seizures in infant rats: ontogeny of behavioral and electrocortical phenomena

Development of the Subfornical Organ and Area postrema of the Male Albino Mouse.Karyometric Effect of Neonatal and Prepuberal Castration

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