Neonatal maternal separation increases the number of p27-positive Sertoli cells in prepuberty

Miyaso, Hidenobu; Takano, Kaiya; Nagahori, Kaoru; Kawata, Sumio; Li, Zhong‐Lian; Kuramasu, Miyuki; Wu, Xi; Ogawa, Yoshiaki; Itoh, Masahiro

doi:10.1016/j.reprotox.2021.03.008

Cited by 2 publications

(7 citation statements)

References 33 publications

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“…In a previous study, we also observed that serum CORT levels increased on PND 10 in NMS mice 26 . Studies have indicated that CORT decreases the level of testosterone, hormone that is important for increasing the Sertoli cell number 30 , 31 .…”

Section: Introductionsupporting

confidence: 52%

“…Jaimes-Hoy et al also documented a decrease in serum thyroid hormone levels on PND 90 as a result of 3-h/day NMS during PND 2–21 29 . Our abovementioned experimental model, i.e., 2-h/day NMS during PND 1–10, demonstrated that serum testosterone levels significantly decreased in NMS mice on PND 10; however, T3 levels did not significantly alter 26 .…”

Section: Introductionmentioning

confidence: 69%

“…In our previous study, we reported that NMS increased the number of p27-positive Sertoli cells and decreased the number of Sertoli cells on PNDs 10 and 16 in response to an increase in serum CORT levels 26 . NMS is a representative model for inducing ELS, and maternal separation results in complex adverse effects, including loss of body temperature and skin-to-skin interaction because of the absence of the maternal mouse, malnutrition, emotional distress, and disturbance of several hormones (such as an increased CORT level), on neonates 25 , 26 . This study demonstrated that CORT administration increased the p27-positive Sertoli cell number and decreased Sertoli cell number on PNDs 10 and 16 (Figs.…”

Section: Discussionmentioning

confidence: 86%

“…Furthermore, NMS reduced the numbers of epididymal spermatozoa and Sertoli cells on postnatal day (PND) 70 25 . Moreover, a previous study demonstrated that NMS decreased the Sertoli cell number on PND 16 and increased the p27-positive Sertoli cell number on PND 10 26 . P27 is a cyclin-dependent kinase (CDK) inhibitor, and elevated p27 expression levels in Sertoli cells have been reported to terminate Sertoli cell proliferation 27 .…”

Section: Introductionmentioning

confidence: 84%

“…Testicular weight, Sertoli cell number, and spermatozoa count in CORT mice on PND 70. Our previous study revealed that the 2-h/day NMS mice demonstrated an approximately three-fold increase in serum CORT level compared with control on PND 10, which decreased testicular weight, Sertoli cell number, and spermatozoa count on PND 70 25,26 . In this study, a similar increase in serum CORT levels was observed in low-dosed-CORT mice as compared with those in control on PND 10 (Fig.…”

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confidence: 93%

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Neonatal corticosterone administration increases p27-positive Sertoli cell number and decreases Sertoli cell number in the testes of mice at prepuberty

Miyaso

Takano

Nagahori

et al. 2022

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Cortisol and corticosterone (CORT) are steroid, antistress hormones and one of the glucocorticoids in humans and animals, respectively. This study evaluated the effects of CORT administration on the male reproductive system in early life stages. CORT was subcutaneously injected at 0.36 (low-), 3.6 (middle-), and 36 (high-dosed) mg/kg body weight from postnatal day (PND) 1 to 10 in ICR mice. We observed a dose-dependent increase in serum CORT levels on PND 10, and serum testosterone levels were significantly increased only in high-dosed-CORT mice. Triiodothyronine levels were significantly higher in the low-dosed mice but lower in the middle- and high-dosed mice. However, testicular weights did not change significantly among the mice. Sertoli cell numbers were significantly reduced in low- and middle-dosed mice, whereas p27-positive Sertoli cell numbers increased in low- and middle-dosed mice. On PND 16, significant increases in testicular and relative testicular weights were observed in all-dosed-CORT mice. On PND 70, a significant decrease in testicular weight, Sertoli cell number, and spermatozoa count was observed. These results revealed that increased serum CORT levels in early life stages could induce p27 expression in Sertoli cells and terminate Sertoli cell proliferation, leading to decreased Sertoli cell number in mouse testes.

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Section: Introductionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 69%