Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk

Rosa, Fernanda; Matazel, Katelin S.; Bowlin, Anne K.; Williams, Keith D.; Elolimy, Ahmed A.; Adams, Sean H.; Bode, Lars; Yeruva, Laxmi

doi:10.3389/fimmu.2020.607609

Cited by 5 publications

(4 citation statements)

References 90 publications

(120 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Myristic acid was also reported to be higher in cecal contents of breastfed piglets (Poroyko et al, 2011). Several free fatty acids including myristic acid, palmitic acid, linoleic acid, linolenic acid, oleic acid, and palmitoleic acid were increased in large intestine contents of piglets fed with human milk, which is in line with our results (Rosa et al, 2020). On the contrary, a different study reported myristic acid to be significantly higher in formula-fed infants (Chow et al, 2014).…”

Section: Increased Fatty Acids In Breastfed Children Reflected Fatty Acid Composition Of Breast Milksupporting

confidence: 91%

Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants

Sillner

Walker

Lucio

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

The early-life metabolome of the intestinal tract is dynamically influenced by colonization of gut microbiota which in turn is affected by nutrition, i.e. breast milk or formula. A detailed examination of fecal metabolites was performed to investigate the effect of probiotics in formula compared to control formula and breast milk within the first months of life in healthy neonates. A broad metabolomics approach was conceptualized to describe fecal polar and semi-polar metabolites affected by feeding type within the first year of life. Fecal metabolomes were clearly distinct between formula- and breastfed infants, mainly originating from diet and microbial metabolism. Unsaturated fatty acids and human milk oligosaccharides were increased in breastfed, whereas Maillard products were found in feces of formula-fed children. Altered microbial metabolism was represented by bile acids and aromatic amino acid metabolites. Elevated levels of sulfated bile acids were detected in stool samples of breastfed infants, whereas secondary bile acids were increased in formula-fed infants. Microbial co-metabolism was supported by significant correlation between chenodeoxycholic or lithocholic acid and members of Clostridia. Fecal metabolites showed strong inter- and intra-individual behavior with features uniquely present in certain infants and at specific time points. Nevertheless, metabolite profiles converged at the end of the first year, coinciding with solid food introduction.

show abstract

Section: Increased Fatty Acids In Breastfed Children Reflected Fatty Acid Composition Of Breast Milksupporting

confidence: 91%

Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants

Sillner

Walker

Lucio

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…decreased in the HM-fed piglets, such as was observed in infants (26) and piglets (59). Among differentially abundant bacterial families, the lower abundance of Campylobacteraceae and the higher abundance of Prevotellaceae were previously observed in the colon of HM-fed piglets (60). Several dietary factors may explain the difference observed in the present study.…”

Section: The Microbiotasupporting

confidence: 66%

“…Moreover, Lactobacillus and Bacteroides were more abundant in HM-fed piglets, which could influence indole metabolism, such as previously reported ( 33 ). Indole-3-propionic acid has been previously shown to be less abundant in HM-fed piglets ( 60 ). Further investigation of the specific HM components shaping the bacterial metabolomic profile is warranted.…”

Section: Discussionmentioning

confidence: 99%

Infant nutrition affects the microbiota-gut-brain axis: Comparison of human milk vs. infant formula feeding in the piglet model

et al. 2022

View full text Add to dashboard Cite

Early nutrition plays a dominant role in infant development and health. It is now understood that the infant diet impacts the gut microbiota and its relationship with gut function and brain development. However, its impact on the microbiota-gut-brain axis has not been studied in an integrative way. The objective here was to evaluate the effects of human milk (HM) or cow’s milk based infant formula (IF) on the relationships between gut microbiota and the collective host intestinal-brain axis. Eighteen 10-day-old Yucatan mini-piglets were fed with HM or IF. Intestinal and fecal microbiota composition, intestinal phenotypic parameters, and the expression of genes involved in several gut and brain functions were determined. Unidimensional analyses were performed, followed by multifactorial analyses to evaluate the relationships among all the variables across the microbiota-gut-brain axis. Compared to IF, HM decreased the α-diversity of colonic and fecal microbiota and modified their composition. Piglets fed HM had a significantly higher ileal and colonic paracellular permeability assessed by ex vivo analysis, a lower expression of genes encoding tight junction proteins, and a higher expression of genes encoding pro-inflammatory and anti-inflammatory immune activity. In addition, the expression of genes involved in endocrine function, tryptophan metabolism and nutrient transport was modified mostly in the colon. These diet-induced intestinal modifications were associated with changes in the brain tissue expression of genes encoding the blood-brain barrier, endocrine function and short chain fatty acid receptors, mostly in hypothalamic and striatal areas. The integrative approach underlined specific groups of bacteria (Veillonellaceae, Enterobacteriaceae, Lachnospiraceae, Rikenellaceae, and Prevotellaceae) associated with changes in the gut-brain axis. There is a clear influence of the infant diet, even over a short dietary intervention period, on establishment of the microbiota-gut-brain axis.

show abstract

“…Lactobacillus species have also been shown to produce indole derivatives that activate aryl-hydrocarbon receptor in CD4 + T cells and promote double positive intraepithelial lymphocytes (IELs) ( Cervantes-Barragan et al, 2017 ). Interestingly, from the same piglet model, we have reported higher levels of indole derivatives in the large intestine of HM group relative to MF ( Rosa et al, 2020 ), suggesting the likely role for this Lactobacillus spp. in maintaining higher levels of indoles in HM.…”

Section: Discussionmentioning

confidence: 63%

Bacterial and Fungal Adaptations in Cecum and Distal Colon of Piglets Fed With Dairy-Based Milk Formula in Comparison With Human Milk

et al. 2022

Self Cite

View full text Add to dashboard Cite

Exclusive breastfeeding is recommended to newborns during the first 6 months of life, whereas dairy-based infant formula is an alternative nutrition source offered to infants. Several studies demonstrated that breastfed infants have a different gut bacterial composition relative to formula-fed infants. In addition, animal models have shown that human milk (HM)–fed piglets had a distinct intestinal bacterial composition compared with milk formula (MF)–fed piglets. However, the gut fungal composition and the interactions with the bacterial community in breastfed compared with formula-fed infants remain to be investigated. In an attempt to evaluate such differences, we used an animal model to perform a shotgun metagenomics analysis on the cecal and distal colon contents of neonatal piglets fed with pasteurized HM or a dairy-based infant formula (MF) during the first 21 days of life. At postnatal day 21 (PND 21), a subset of piglets from each diet group (n = 11 per group) was euthanized. The remaining piglets in each group were weaned to a solid diet and euthanized at PND 51 (n = 13 per group). Large intestine contents (i.e., cecum and distal colon) were subjected to shotgun metagenomics analysis. The differential taxonomic composition of bacteria and fungi and the predicted functional gene profiling were evaluated. Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria are the most abundant bacterial phyla observed in piglets at PND 21 and PND 51. In the large intestine at PND 21 and PND 51, Proteobacteria phylum was significantly higher in MF-fed group, and species Burkholderiales bacterium of phyla was significantly higher in MF group relative to HM group. In addition, in HM group, several Lactobacillus spp. and Bacteroides spp. were higher relative to MF group in the large intestine at PND 21 and PND 51. Fungal genus Aspergillus was higher in MF, whereas Malassezia was lower relative to HM group. Persistent effects of the neonatal diets were observed at PND 51, where alpha- and beta-diversity differences were detected for bacterial and fungal species in the large intestine. Overall, our findings indicate that neonatal diet affects the large intestinal microbial community during the exclusive milk-feeding period, as well as after the introduction of the complementary food.

show abstract

Neonatal Diet Impacts the Large Intestine Luminal Metabolome at Weaning and Post-Weaning in Piglets Fed Formula or Human Milk

Cited by 5 publications

References 90 publications

Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants

Longitudinal Profiles of Dietary and Microbial Metabolites in Formula- and Breastfed Infants

Infant nutrition affects the microbiota-gut-brain axis: Comparison of human milk vs. infant formula feeding in the piglet model

Bacterial and Fungal Adaptations in Cecum and Distal Colon of Piglets Fed With Dairy-Based Milk Formula in Comparison With Human Milk

Contact Info

Product

Resources

About