Neomycin-Induced Hybrid Triplex Formation

Arya, Dev P.; Coffee, R. Lane; Charles, I.

doi:10.1021/ja016481j

Cited by 93 publications

(89 citation statements)

References 28 publications

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“…Therefore, cryptolepine constitutes a novel triplex stabilizing agent. Several compounds like a tricationic cyanine dye [51] or neomycine [52] have already been described as poly(dA)/2poly(dT) triplex ligands. Interestingly, a structural similarity can be established between cryptolepine and the tetracyclic benzopyridoindole compounds BePI and BgPI which potently stabilize triplex DNA structures [15,53].…”

Section: Discussionmentioning

confidence: 99%

Interactions of cryptolepine and neocryptolepine with unusual DNA structures

et al. 2003

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1 These authors contributed equally to this work. AbstractCryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a preference for GC-rich sequences. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of cryptolepine and neocryptolepine for DNA structures among duplexes, triplexes, quadruplexes and single strands. Our data confirm that cryptolepine and neocryptolepine prefer GC over AT-rich duplex sequences, but also recognize triplex and quadruplex structures. These compounds are weak telomerase inhibitors and exhibit a significant preference for triplexes over quadruplexes or duplexes.

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Section: Discussionmentioning

confidence: 99%

Interactions of cryptolepine and neocryptolepine with unusual DNA structures

et al. 2003

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“…There is, however, little information available for antibiotics that selectively bind DNA triplex grooves or RNA triplex grooves. We have recently reported on the importance of neomycin in narrowing the disparity between groove recognition of duplex versus triplex nucleic acids [113,114]. Neomycin was shown as one of the first examples that bridge this gap and thus may lead to a novel understanding of the recognition principle(s) involved in selective targeting of triplex grooves.…”

Section: The Nucleic Acid Triplex: Role Of Aminoglycosidesmentioning

confidence: 99%

Aminoglycoside–Nucleic Acid Interactions: The Case for Neomycin

Arya

2005

Topics in Current Chemistry

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“…There is extensive evidence describing the ability of aminoglycosides (e.g. neomycin) to bind both duplex and triplex forms of DNA, in addition to quadruplex structures, DNA:RNA hybrids, and to some extent, single-stranded nucleic acids [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Molecular recognition of single‐stranded RNA: Neomycin binding to poly(A)

Gray

Kumar

et al. 2009

FEBS Letters

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Edited by Hans EklundKeywords: RNA recognition Aminoglycoside Neomycin Poly(A) DNA RNA a b s t r a c t Poly(A) is a relevant sequence in cell biology due to its importance in mRNA stability and translation initiation. Neomycin is an aminoglycoside antibiotic that is well known for its ability to target various nucleic acid structures. Here it is reported that neomycin is capable of binding tightly to a single-stranded oligonucleotide (A 30 ) with a K d in the micromolar range. CD melting experiments support complex formation and indicate a melting temperature of 47°C. The poly(A) duplex, which melts at 44°C (pH 5.5), was observed to melt at 61°C in the presence of neomycin, suggesting a strong stabilization of the duplex by the neomycin.

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Neomycin-Induced Hybrid Triplex Formation

Cited by 93 publications

References 28 publications

Interactions of cryptolepine and neocryptolepine with unusual DNA structures

Interactions of cryptolepine and neocryptolepine with unusual DNA structures

Aminoglycoside–Nucleic Acid Interactions: The Case for Neomycin

Molecular recognition of single‐stranded RNA: Neomycin binding to poly(A)

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