Neogenesis ofEthanol and Fusel Oils in Putrefying Blood

Huckenbeck, W.

doi:10.1007/978-1-59259-921-9_9

Cited by 5 publications

(3 citation statements)

References 121 publications

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“…Many experiments on animals or in vitro have been made and published concerning pharmacokinetics of fusel oils, establishing that the distribution volume for methanol, 1-propanol and 2-butanol is similar to the one for ethanol [14] while isobutanol distributes in a more larger one, including fatty tissue [15]. Elimination rates are different among congeners, for example half-time in blood is in average 2 ¼ hours for isobutanol and 3 ½ hours for 1-propanol [2], therefore low amounts of isobutanol may invalidate the assumption of recent alcohol ingestion.…”

Section: Volatile Congeners In Alcoholic Beverages: Analysis and Forementioning

confidence: 99%

“…However, in our samples of home-made Romanian plum spirits we found concentrations of 1-butanol between 8 -74 mg/l, thus its presence signification in a blood sample must be interpreted carefully. Identification of methanol in the blood specimen is definitely meaning alcohol consumption before collecting and depositing the sample [14].…”

Section: Volatile Congeners In Alcoholic Beverages: Analysis and Forementioning

confidence: 99%

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Volatile congeners in alcoholic beverages: analysis and forensic significance

Jung¹,

Jung²,

Auwärter³

et al. 2010

RJLM

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Beside ethyl alcohol, the major active component of alcoholic beverages, almost all drinks contain volatile and non-volatile substances called congeners. They have various pharmacological effects and are present in different concentrations depending on beverage type and manufacturing methods. We analyzed volatile congeners in five samples of Romanian home-made plum spirits collected from four Transylvanian counties were double distillation is used after fruits fermentation, by a GC method developed in the Institute of Legal Medicine from Freiburg. Volumetric concentration of ethanol ranged between 52 and 76%. Calibration with aqueous standards resulted in linearity with a correlation coefficient of over 0.995 for each congener. We found high amounts of isobutanol in our samples compared to beverages listed in the literature, influencing forensic opinion concerning the time of ingestion in correlation with concentration of 1.propanol. Another congener, usually absent in alcoholic beverages, 1-butanol, was constantly present in all our spirit samples, meaning a limitation of its use as a blood putrefaction marker. Volatile congener composition of all our samples complied with EEC regulations with regard to fruit spirits.

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Section: Volatile Congeners In Alcoholic Beverages: Analysis and Forementioning

confidence: 99%

Section: Volatile Congeners In Alcoholic Beverages: Analysis and Forementioning

confidence: 99%

Volatile congeners in alcoholic beverages: analysis and forensic significance

Jung¹,

Jung²,

Auwärter³

et al. 2010

RJLM

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“…A common defense challenge in court purporting that the measured blood ethanol concentration does not represent the driver's blood ethanol concentration at the time of the blood draw is the assertion that ethanol was formed in the blood tube after the blood draw [2,3]. In vitro formation of ethanol has been referred to as both neoformation and neogenesis [4][5][6]. This challenge is based on the claim that the blood was contaminated with Candida albicans (C. Albicans) that converted glucose in the blood into ethanol.…”

Section: Introductionmentioning

confidence: 99%

Lack of fermentation in antemortem blood samples stored unstoppered in various locations

Kosecki

Brooke

Raines

2022

Journal of Forensic Sciences

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A common defense challenge when antemortem blood ethanol results are presented at trial is the assertion that ethanol was formed in the blood tube after the blood draw through fermentation of the blood glucose by Candida albicans (C. Albicans). In contrast, decades of research into the stability of ethanol in antemortem blood collected for forensic purposes have consistently shown that any analytically significant change in ethanol concentration is a decrease and initially, ethanol-negative blood remains ethanol-negative with storage. For there to be any possibility of fermentation to occur by C. Albicans in an antemortem blood sample there must be a plausible mechanism for introduction of C. Albicans into the blood. One mechanism proffered at trial is environmental contamination resulting from ambient air drawn into the evacuated blood collection tube. Blood was drawn from ethanol-free individuals into 6 and 10-ml graytop Vacutainer® tubes containing sodium fluoride and 6-ml Vacutainer® tubes without a preservative. Following the blood draws, the tubes were stored unstoppered at room temperature for 24 or 48 h in various locations. Following unstoppered storage, the tubes were stoppered and stored refrigerated (~4°C), left at room temperature (~22°C), or placed in an oven (37°C). The refrigerated blood was analyzed for ethanol using headspace gas chromatography after both 5 days and 32 months. Unrefrigerated blood samples were analyzed after being stored at room temperature or in an oven for up to 30 days. Ethanol was not detected in any of the blood tubes after storage regardless of storage time, storage temperature, or preservative concentration.

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