Nemesia Root Hair Response to Paper Pulp Substrate for Micropropagation

Labrousse, Pascal; Delmail, David; Decou, Raphaël; Carlué, Michel; Lhernould, Sabine; Krausz, Pierre

doi:10.1100/2012/859243

Cited by 11 publications

(5 citation statements)

References 45 publications

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“…Moreover, kinetin could be also tested on N. triphylla shoot propagation as 1 mg.dm -3 of this cytokinin in the culture medium increased the shoot number of Withania somnifera (Sabir et al 2008). As previously developed for other species (Labrousse et al 2012), in vitro rooting and acclimation assays are currently in progress to establish a complete in vitro to ex vitro regeneration protocol for this species. It should be noted that, as in the case of Swertia chirayita described by Joshi and Dhawan (2007), it would be useful to assess the genetic fidelity of micropropagated plants to limit the impact of somaclonal variation on this endangered species.…”

Section: Resultsmentioning

confidence: 99%

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

Delmail

Labrousse

Lesouëf³

et al. 2012

Braz. arch. biol. technol.

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Section: Resultsmentioning

confidence: 99%

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

Delmail

Labrousse

Lesouëf³

et al. 2012

Braz. arch. biol. technol.

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“…plantlets were potted on peat moss alone or in combination with sand at (1:1 v: v), the stems of the in vitro acclimatized plants were allowed to grow up to the highest value [33]. Also, Labrousse et al [34] reported that the use of peat moss as a substrate in vitro culture enhanced rooting and increased the number and length of roots. for the antioxidant potential in B. pauciflora.…”

Section: Acclimatizationmentioning

confidence: 99%

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Sayed

Marzouk²,

Sokkar

2022

Egypt. J. Chem.

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The present study considered the in vitro propagation of Brunfelsia pauciflora utilizing various cytokinins, as well the estimation of the antioxidant, total phenolics (TPs), and flavonoids (TFs) for shootlets of each treatment. The explants were sterilized with 0.2% mercuric chloride (20 min) and then cultured on Murashige and Skoog medium (MS) supplemented with three cytokinins; benzyl aminopurine (BAP), 6-Furfuryl-aminopurine (Kin), and isopentenyl adenine (2iP) at different concentrations. The highest shootlets number and fresh weight were obtained using 1.0 mg/L BAP, while shootlets length is increased using 2.0 mg/L kin. Additionally, significant numbers of leaves were observed for the plantlets supplemented with kin (1.0 & 3.0 mg/L) and 2iP (2.0 & 3.0 mg/L). The shootlets were rooted one month later with different strengths (full, ¾, ½, or ¼) on MS media without or with 1.0g/L activated charcoal (AC). Shootlet cultured on full-strength MS medium with AC gave the longest root, while the greatest numbers of roots were recorded using ¾ strength without AC. All the rooted shootlets were acclimatized on peat moss and recorded with the longest root length and greatest leaves and roots numbers. Alternatively, the methanolic extract of shootlets supplemented with 2iP (3.0 mg/L) yielded the maximum antioxidant effect (89.89 ± 0.28 mg Trolox /g extract), TPs (57.574±0.82 mg Gallic/g extract), and TFs (46.146 ± 0.31 mg Catechin/g extract) compared with the control (MS free) and mother plant. In conclusion, the antioxidant potential was shown to be strongly correlated with the TFs and the number of leaves /shootlet.

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“…It is known that in in vitro conditions, the propagules are cultured in test tubes or vessels under aseptic conditions, low light irradiance and high relative humidity [75]. Besides, the culture medium contains nutrients and sugar, and thus the propagules are usually grown heterotrophically since any roots formed are not always functional due to absence of root hairs [74,76]. The results of this study showed that the adaptation of young plantlets to the new environment should be carried out gradually and carefully.…”

Section: Rooting Of Shoots and Acclimatization Of Plantletsmentioning

confidence: 99%

Conservation, Regeneration and Genetic Stability of Regenerants from Alginate-Encapsulated Shoot Explants of Gardenia jasminoides Ellis

et al. 2021

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The present study demonstrates the potential of the alginate encapsulation of shoot tips and nodal segments of Gardenia jasminoides Ellis, the short-term cold storage of artificial seeds and subsequent successful conversion to desirable, uniform and genetically stable plantlets. Shoot tips and first-node segments below them, derived from shoots of in vitro cultures, responded better than second-to-fourth-node segments on agar-solidified Murashige and Skoog (MS) nutrient medium and thus, they were used as explants for alginate encapsulation. Explant encapsulation in 2.5% sodium alginate in combination with 50 mM of calcium chloride resulted in the production of soft beads, while hardening in 100 mM of calcium chloride formed firm beads of uniform globular shape, suitable for handling. The addition of liquid MS nutrient medium in the sodium alginate solution doubled the subsequent germination response of the beads. The maintenance of alginate beads under light favored their germination response compared to maintenance in darkness. Encapsulated shoot tip explants of gardenia, which were stored at 4 °C for 4, 8 or 12 weeks, showed a gradual decline in their regeneration response (73.3, 68.9, 53.3%, respectively), whereas, non-encapsulated explants (naked), stored under the same time durations of cold conditions, exhibited a sharp decline in regeneration response up to entirely zeroing (48.9, 11.1, 0.0%, respectively). Shoots, derived from 12-week cold-stored encapsulated explants, were easily rooted in solid MS nutrient medium with the addition of 0.5 μM of Indole-3-acetic acid (IAA) and after transplantation of the rooted plantlets individually to pots containing a peat–perlite (3:1, v/v) substrate, they were successfully acclimatized in the greenhouse under the gradual reduction of 75 or 50% shading with survival rates of 95–100%. The genetic stability of the acclimatized plantlets was assessed and compared with the mother plant using inter simple sequence repeat (ISSR) markers. ISSR analysis confirmed that all regenerated plantlets were genetically identical to the mother plant. This procedure of artificial seed production could be useful for the short-term storage of germplasm and the production of genetically identical and stable plants as an alternative method of micropropagation in Gardenia jasminoides.

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Nemesia Root Hair Response to Paper Pulp Substrate for Micropropagation

Cited by 11 publications

References 45 publications

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Conservation, Regeneration and Genetic Stability of Regenerants from Alginate-Encapsulated Shoot Explants of Gardenia jasminoides Ellis

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