Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

Monroy, Francisco; Adams, J.H.; Dobson, Colin; East, I.J.

doi:10.1016/0014-4894(89)90009-x

Cited by 17 publications

(5 citation statements)

References 15 publications

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“…However, given the statistical significance of these results together with the low level of variation in adult worm burdens in Group I1 compared to the other three groups, it is clear that the combination of b e r y l l i~ and surface extracts has a major significant effect which is not due to the adjuvant alone. Similar dependence of the protective ability of nematode antigens on the immunomodulatory action of various adjuvants has been recently reported by Monroy et al (1989) and Wedrychowicz & Bezubik (1990). Interestingly, Adams (1989) reported that Freund's complete adjuvant increased Comparative analysis of serum antibody isotypes reacting with nematode antigens revealed that: first, a significant IgA response to surface antigcns was elicited only in the animals immunized with beryllium hydroxide and L3 surface extract; secondly, an IgM response to the surface was elicited in all three immunized groups, and thirdly all three vaccinated groups showed a high IgG response to the surface antigens, but with Group I1 showing the slowest rise in titre.…”

Section: Discussionsupporting

confidence: 84%

Immune responses of sheep to surface antigens of infective larvae of Ostertagia circumcincta

Wędrychowicz

Bairden

Tait

et al. 1992

Parasite Immunology

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Immune responses to surface antigens of infective larvae of Ostertagia circumcincta were studied in 5-month old Finn-Dorset male lambs. The sheep were vaccinated subcutaneously with 2 doses of 25 micrograms/kg body weight of infective larvae surface or somatic extracts and Freund's or beryllium hydroxide adjuvants. It was found that only in lambs vaccinated with L3 surface extracts and beryllium hydroxide as an adjuvant did worm burdens differ significantly (P less than 0.01) from those of challenge controls (71.7% protection). Sheep vaccinated with the same antigenic preparation but administered with Freund's adjuvant or with beryllium adjuvant and somatic extracts demonstrated rather poor protection (32.4 and 30.5% respectively). All the vaccinated sheep showed a high IgG response to L3 surface antigens while significant levels of serum and bile IgA reacting with the surface extracts were only detected in those lambs vaccinated with L3 surface extracts and beryllium hydroxide. In immunofluorescence tests serum IgA of this group reacted with the whole surface of exsheathed larvae or with the site of opening of excretory pore while IgG antibodies reacted strongly with the anterior and posterior parts of the infective larvae. Both IgA and IgG responses to surface antigens were stage specific.

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Section: Discussionsupporting

confidence: 84%

Immune responses of sheep to surface antigens of infective larvae of Ostertagia circumcincta

Wędrychowicz

Bairden

Tait

et al. 1992

Parasite Immunology

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“…When soluble antigens previously used as im munogens were used for the antibody binding, there was a predominance of specific IgGl which was de tected in sera of mice immunized with either of the two assayed antigens. These results are in good agree ment with those where B. pertussis used as adjuvant was able to stimulate all IgG isotype antibodies but predominantly IgGl [19], When formaldehyde-treated EPI were used for the ELISA, not only IgGl but also IgG2 were detected. Previous studies have show that F IV and Eas epitopes are also expressed on the para site cell surface [11,13].…”

Section: Discussionsupporting

confidence: 78%

IgG Isotype Profiles Induced in Mice by Two <i>Trypanosoma</i><i>cruzi</i> Electronegative Antigens

Cerbán

Gruppi²,

Gea³

et al. 1991

Int Arch Allergy Immunol

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In this work we studied the IgG isotypes induced in mice immunized with two Trypanosoma cruzi acidic antigenic fractions (F IV and Eas 4.5) and the level of protection to a later infection with parasites. F IV is a cytosolic antigen from epimastigotes, and Eas 4.5 is an exoantigen released by trypomastigotes. The most relevant epitopes of Eas 4.5 are carbohydrates. A high prevalence of IgG1, low levels of IgG3 and no IgG2 antibodies against F IV and Eas 4.5 were found in sera obtained 2 weeks after the last antigen dose from animals immunized with F IV (group I) or Eas 4.5 (group II). Immunized mice from both groups were infected with trypomastigotes, and the parasitemias detected later on were significantly lower than in control groups (p < 0.01, group I; p < 0.001, group II). The amount of IgG2-specific antibodies, which was only detected using epimastigotes as antigen in ELISA, was significantly increased after the infection, but no major changes were seen in the profiles of other isotypes.

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“…In retrospect, this may have compromised Th2 priming, because in our hands HES administered in alum induces complete sterilising immunity (Hewitson, et al, 2011a). Indeed, a separate study reported that alum was the most effective adjuvant to induce immunity to H. polygyrus after immunisation with immunoprecipitated proteins from multiply infected mice (Monroy, et al, 1989a). As with most other helminths, irradiated larval vaccines have been tested (Pleass and Bianco, 1996, Maizels, et al, 1999); interestingly, 25-kRad irradiated larvae effectively induce immunity to challenge, but not if co-administered with non-irradiated parasites: the unaffected organisms are thus able to suppress any protective effect engendered by the attenuated larvae (Behnke, et al, 1983), and support the contention that adult worms are able to negate the development of protective immunity.…”

Section: H Polygyrus As a Model For Anti-nematode Vaccinesmentioning

confidence: 99%

Immune modulation and modulators in Heligmosomoides polygyrus infection

Maizels

Hewitson

Murray

et al. 2012

Experimental Parasitology

111

105

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The intestinal nematode parasite Heligmosomoides polygyrus bakeri exerts widespread immunomodulatory effects on both the innate and adaptive immune system of the host. Infected mice adopt an immunoregulated phenotype, with abated allergic and autoimmune reactions. At the cellular level, infection is accompanied by expanded regulatory T cell populations, skewed dendritic cell and macrophage phenotypes, B cell hyperstimulation and multiple localised changes within the intestinal environment. In most mouse strains, these act to block protective Th2 immunity. The molecular basis of parasite interactions with the host immune system centres upon secreted products termed HES (H. polygyrus excretory-secretory antigen), which include a TGF-β-like ligand that induces de novo regulatory T cells, factors that modify innate inflammatory responses, and molecules that block allergy in vivo. Proteomic and transcriptomic definition of parasite proteins, combined with biochemical identification of immunogenic molecules in resistant mice, will provide new candidate immunomodulators and vaccine antigens for future research.

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Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

Cited by 17 publications

References 15 publications

Immune responses of sheep to surface antigens of infective larvae of Ostertagia circumcincta

Immune responses of sheep to surface antigens of infective larvae of Ostertagia circumcincta

IgG Isotype Profiles Induced in Mice by Two <i>Trypanosoma</i><i>cruzi</i> Electronegative Antigens

Immune modulation and modulators in Heligmosomoides polygyrus infection

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