Nematophagous fungi in sheep faeces in Minas Gerais, Brazil

Saumell, Carlos; Padilha, Terezinha; Santos, Clóvis de Paula

doi:10.1017/s095375629900218x

Cited by 14 publications

(7 citation statements)

References 17 publications

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“…Sapróbia, foi isolada de musgos (Domsch et al 1993) e de Miconia cabussu Hoehne (Gusmão et al 2001). Sua atuação como predadora de nematóides é bem conhecida (Domsch et al 1993;Saumell et al 2000). Distribuição geográfica: cosmopolita (Ellis 1971;Nelson 1964).…”

Section: Resultsunclassified

“…Distribuição geográfica: Brasil (Saumell et al 2000), Estados Unidos da América (Drechsler 1950), Peru (Matsushima 1993).…”

Section: Humicola Griseaunclassified

“…Encontrado abundantemente nos solos e sobre folhedo em decomposição, está amplamente distribuído (Kirk et al 2001). Esta e outras espécies do gênero foram relatadas no Brasil, em pesquisas visando o controle de nematóides (Dalla Pria et al 1991;Saumell et al 2000). …”

Section: Humicola Griseaunclassified

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Hyphomycetes (fungos conidiais) associados a briófitas em decomposição

2008

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Section: Resultsunclassified

“…Distribuição geográfica: Brasil (Saumell et al 2000), Estados Unidos da América (Drechsler 1950), Peru (Matsushima 1993).…”

Section: Humicola Griseaunclassified

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Hyphomycetes (fungos conidiais) associados a briófitas em decomposição

2008

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“…The fungus was maintained in Petri dishes (10 cm diameter) containing water agar at 25°C for 7 days (Saumell et al 2000). Four circles (5 mm diameter) of water agar were removed in equidistant areas of eight Petri dishes containing cultured D. flagrans and 480 L 3 or Panagrellus sp.…”

Section: Light and Scanning Electron Microscopymentioning

confidence: 99%

Kinetics of capture and infection of infective larvae of trichostrongylides and free-living nematodes Panagrellus sp. by Duddingtonia flagrans

et al. 2011

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Duddingtonia flagrans, a nematode-trapping fungus, has been investigated as an agent for biological control against infective larvae of gastrointestinal nematode parasites of production animals. The initial process of nematode-trapping fungi infection is based on an interaction between the trap structure of the fungus and the surface of the nematode cuticle. This report investigates by light and scanning electron microscopy the kinetics of capture and infection during the interaction of D. flagrans with the infective larvae (L(3)) of trichostrongylides and the free-living nematode Panagrellus sp. D. flagrans was cultivated for 7 days in a Petri dish containing agar-water. L(3) and Panagrellus sp. were inoculated in the Petri dishes and the samples consisting of agar-L(3)-fungi and agar-Panagrellus sp.-fungi were collected after 10, 20, 30, 40, 50, 60, and 70 min and 3, 4, 5, 10, 15, 20, and 25 h of interaction. All samples were observed by light microscopy. The samples with 1, 5, 15, and 25 h of interaction were also analyzed by scanning electron microscopy. The interaction was monitored up to 25 h. An initial differentiation of predation structures was observed after 30 min of interaction. The presence of traps and of captured L(3) or Panagrellus sp. occurred after 70 min. The live captured nematodes were observed up to 3 h of interaction. However, after 4 h, all Panagrellus sp. were dead. It took 15 h of interaction for the fungus to invade the L(3), and the presence of hyphae inside the nematode near the region of penetration was evident. At this time, the hyphae had filled the whole body of Panagrellus sp. The complete occupation of the body of L(3) occurred at 20 h of interaction and with 25 h the nematode was completely damaged except for the cuticle. Although the double cuticle of L(3) slows the penetration of D. flagrans, it was possible to verify that the process of trap formation and capture occurs quickly when both nematodes were tested, suggesting that the organisms would eventually be killed once in contact with the fungi encouraging the use of the fungus as a biological control agent.

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“…The nematode was obtained as a gift from Dr. Paulo D. Castellane from FCA/Unesp. The fungus was maintained in Petri plates containing water agar (Saumell et al, 2000) and the nematode in Erlenmeyer flask containing oat-agar (Heintz 1978). Acid phosphatase activity was evaluated in samples from: (a) fungus alone, (b) nematode alone, and fungus-nematode interaction (c) directly in liquid medium or (d) first in solid and later in liquid medium.…”

Section: Production and Sample Preparation Of D Flagrans Panagrellumentioning

confidence: 99%

Acid phosphatase activity during the interaction of the nematophagous fungus Duddingtonia flagrans with the nematode Panagrellus sp

Cruz

Silva

Carneiro³

et al. 2009

Journal of Invertebrate Pathology

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The use of Duddingtonia flagrans, a nematode-trapping fungus, has been investigated as a biological control method against free living larvae of gastrointestinal nematodes of livestock animals. This fungus captures and infects the nematode by cuticle penetration, immobilization and digestion of the internal contents. It has been suggested that this sequence of events occurs by a combination of physical and enzymatical activities. This report characterizes the acid phosphatase activity during the interaction of D. flagrans with the free-living nematode Panagrellus sp. The optimum pH for the hydrolysis of the acid phosphatase substrate p-nitrophenyl phosphate was 2.2, 2.8 and 5.4 from D. flagrans alone and 2.2 and 5.4 for Panagrellus sp alone, fungus-nematode interaction in liquid medium and fungus-nematode interaction in solid medium. Different acid phosphatase activity bands were detected by SDS-PAGE. Maximum acid phosphatase activity of the fungus or nematode alone and of the fungus-nematode interaction occurred within 70min of incubation in the presence of the substrate 4-methylumbelliferyl phosphate. The activity of this enzyme was significantly higher for the fungus-nematode interaction when compared to the organisms alone, indicating a synergistic response. Furthermore, structures appeared in the hyphae after 30min, nematodes were observed adhered after 40min and many were captured by the typical fungus traps after 70min of interaction. The participation of acid phosphatase activity and its importance during the interaction of the fungus with the nematode were discussed.

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Nematophagous fungi in sheep faeces in Minas Gerais, Brazil

Cited by 14 publications

References 17 publications

Hyphomycetes (fungos conidiais) associados a briófitas em decomposição

Hyphomycetes (fungos conidiais) associados a briófitas em decomposição

Kinetics of capture and infection of infective larvae of trichostrongylides and free-living nematodes Panagrellus sp. by Duddingtonia flagrans

Acid phosphatase activity during the interaction of the nematophagous fungus Duddingtonia flagrans with the nematode Panagrellus sp

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