Nematodes avoid and are killed by Bacillus mycoides-produced styrene

Luò, Tāo; Hou, Shifeng; Li, Yang; Qi, Gaofu; Zhao, Xiuyun

doi:10.1016/j.jip.2018.09.006

Cited by 23 publications

(20 citation statements)

References 40 publications

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“…Altogether these results support the hypothesis that the effect could be attributed to some potentially unknown nematicidal compounds as those recently discovered by several authors (Gao et al, 2016;Geng et al, 2017;Huang et al, 2018;Luo et al, 2018;Zeng et al, 2015;Zheng et al, 2018) In the present work, five endophytic bacteria isolates (CCBLR15, CCBLR14, CCBLR1, CCBLR13, and CCBMTR4) all belonging to the Bacillus genus were able to lead to 100% of mortality on both R. duriophilus and P. coffeae. The analysis of their 16S rDNA sequences revealed that these isolates shared between 98.7% and up to 100% identity with sixteen species all belonging to the Bacillus cereus sensu lato group including B. albus, B.…”

Section: Discussionsupporting

confidence: 93%

Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities

Duong

Nguyen²,

Phan³

et al. 2021

Microbiological Research

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The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea canephora and Coffea liberica, twelve were reported for the first time in endophytic association with coffee including members of the genera Brachybacterium, Caballeronia, Kitasatospora, Lechevalieria, Leifsonia, Luteibacter, Lysinibacillus, Mycolicibacterium, Nakamurella, Paracoccus, Sinomonas and Sphingobium. A total of eighty bacterial endophytes were characterized in vitro for several plant growth promoting and biocontrol traits including: the phosphate solubilization, the indolic compounds, siderophores, HCN, esterase, lipase, gelatinase and chitinase production. A subset of fifty selected bacteria were tested for their potential as biocontrol agents with in vitro confrontations with the fungal pathogen Fusarium oxysporum as well as the coffee parasitic nematodes Radopholus duriophilus and Pratylenchus coffeae. The three most efficient isolates on F. oxysporum belonging to the Bacillus, Burkholderia, and Streptomyces genera displayed a growth inhibition rate higher than 40%. Finally, five isolates from the Bacillus genus were able to lead to 100% of mortality in 24 h on both R. duriophilus and P. coffeae.

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Section: Discussionsupporting

confidence: 93%

Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities

Duong

Nguyen²,

Phan³

et al. 2021

Microbiological Research

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“…Styrene and derivatives have been found in plants, including soybean and as a by-product of fungal and microbial metabolism (Shirai and Hisatsuka, 1979 ; Arpaia et al, 2011 ; Kim et al, 2020 ). Styrene produced by Bacillus mycoides in the rhizosphere of tomato plants exhibited high nematocidal activity against M. incognita (Luo et al, 2018 ). Butylated Hydroxytoluene (CPV76), a phenylpropanoid and benzanoid derivative, is a natural antioxidant, and has been found in plant oils (Yehye et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Soybean Cyst Nematodes Influence Aboveground Plant Volatile Signals Prior to Symptom Development

Constantino

Şennik

et al. 2021

Front. Plant Sci.

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Soybean cyst nematode (SCN), Heterodera glycines, is one of the most destructive soybean pests worldwide. Unlike many diseases, SCN doesn't show above ground evidence of disease until several weeks after infestation. Knowledge of Volatile Organic Compounds (VOCs) related to pests and pathogens of foliar tissue is extensive, however, information related to above ground VOCs in response to root damage is lacking. In temporal studies, gas chromatography-mass spectrometry analysis of VOCs from the foliar tissues of SCN infested plants yielded 107 VOCs, referred to as Common Plant Volatiles (CPVs), 33 with confirmed identities. Plants showed no significant stunting until 10 days after infestation. Total CPVs increased over time and were significantly higher from SCN infested plants compared to mock infested plants post 7 days after infestation (DAI). Hierarchical clustering analysis of expression ratios (SCN: Mock) across all time points revealed 5 groups, with the largest group containing VOCs elevated in response to SCN infestation. Linear projection of Principal Component Analysis clearly separated SCN infested from mock infested plants at time points 5, 7, 10 and 14 DAI. Elevated Styrene (CPV11), D-Limonene (CPV32), Tetradecane (CPV65), 2,6-Di-T-butyl-4-methylene-2,5-cyclohexadiene-1-one (CPV74), Butylated Hydroxytoluene (CPV76) and suppressed Ethylhexyl benzoate (CPV87) levels, were associated with SCN infestation prior to stunting. Our findings demonstrate that SCN infestation elevates the release of certain VOCs from foliage and that some are evident prior to symptom development. VOCs associated with SCN infestations prior to symptom development may be valuable for innovative diagnostic approaches.

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“…The LC50 and LC90 values of acetophenone were calculated. Assay for nematode avoidance of acetophenone was conducted as our previous study [32]. Water-agar medium (1.5% agar) was prepared in 10-cm plates.…”

Section: Analysis Of Nematicidal Activity Of Metabolitesmentioning

confidence: 99%

Intergrated Metagenomics and Metabolomics Analysis Discovers Nematicidal Microbes, Enzymes and Metabolites From the Plant Rhizosphere Microbiota

Lin

Yuan

Ruan

et al. 2021

Preprint

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BackgroundRoot-knot nematode Meloidogyne incognita infects root systems of many crops resulting in huge decrease of crop production. Nematicidal microorganisms provides a safe and effective strategy to control M. incognita infection. In order to find more microorganisms with high activity and new nematicidal metabolites, we collected the M. incognita infected tobacco rhizosphere soils (RNI) and non-infected tobacco rhizosphere soils (NS), and investigated their microbial community and network via metagenomics and metabolomics analysis. ResultsMicrobial networks of RNI soils were very different from the NS soils. Many nematicidal microorganisms were enriched in the NS soils, including some isolates such as Aspergillus , Achromobacter , Acinetobacter , Bacillus , Burkholderia , Comamonas , Enterobacter , Lysobacter , Microbacterium , Paenibacillus , Pantoea , Pseudomonas , Streptomyces and Variovorax. Enzymes analysis showed these nematicidal microorganisms can produce proteases, chitinase and lipases. The functions genes belonging to pathways of secondary metabolites biosynthesis and carbohydrate transport and metabolism were overrepresented in the rhizophere microbiota of NS soils comparing with the RNI soils. 102 metabolites contents were significantly different between the RNI and NS rhizosphere microbiota. 35 metabolites were overrepresented in the NS soils comparing the RNI samples, including acetophenone. Acetophenone showed high nematicidal (LC 50 = 0.66 μg/ml) and avoidance activity against M. incognita . A isolate of Bacillus amyloliquefaciens W1 with production of acetophenone can kill 98.8% of M . incognita . ConclusionsIn general, the rhizophere microbiota of NS soils could produce volatile materials, multiple enzymes and secondary metabolites against nematode. Collectively, the microbiota of NS and RNI rhizophere differed significantly in microbial network structure, community composition, function genes and metabolites. Collectively, combination of multi-omics analysis and culture-dependent technology is powerful for finding nematicidal microorganisms and metabolites from soil.

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Nematodes avoid and are killed by Bacillus mycoides-produced styrene

Cited by 23 publications

References 40 publications

Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities

Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities

Soybean Cyst Nematodes Influence Aboveground Plant Volatile Signals Prior to Symptom Development

Intergrated Metagenomics and Metabolomics Analysis Discovers Nematicidal Microbes, Enzymes and Metabolites From the Plant Rhizosphere Microbiota

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