Nematicidal natural products from the aerial parts ofRubus niveus

Abstract

Studies on the aerial parts of Rubus niveus yielded six known compounds, 3,5-dihydroxy benzoic acid C(7)H(6)O(4), (1), gallic acid C(7)H(6)O(5) (2), ethyl galactoside (3), oleanolic acid (4), beta-sitosterol (5) and 3-O-[beta-D-galactopyranosyl-(12)-D-glucopyranoside (6). Besides this, a gallic acid derivative with methyl substitution was synthesised as tetramethyl gallate (3). Together with this derivative, compounds 1, 2, the alcohol soluble, chloroform soluble and petroleum ether soluble extracts of the aerial parts of R. niveus were screened for its nematicidal activity against freshly hatched second stage juveniles of Meloidogyne incognita (root-knot nematode), exhibiting 100, 94, 100, 52, 45 and 14% mortality, respectively of M. incognita after 48 h at 0.5% concentration. Compounds 1, 2 and 3 were found to be more potent than the nematicide Azadirachta indica at the same concentration. Negative results were obtained for nematicidal activity of the petroleum ether extract of R. niveus leaf extract. This is the first report on the isolation of chemical constituents as well as the nematicidal activity of compounds and any part of R. niveus.

Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita

“…Also, the mortality of M. incognita J2 was 100% after 12 h incubation with 1.0 mg/ml of gallic acid purified from TNB [52]. According to Sultana et al [53], 3,5-DHBA purified from the aerial parts of Rubus niveus show strong nematicidal activity against M. incognita, exhibiting 100% mortality of M. incognita after 48 h at 5 mg/ml. In this study, nematicidal activity was also determined for the 3,4-DHBA purified from TNB.…”

Genome Sequence of Delftia acidovorans HK171, a Nematicidal Bacterium Isolated from Tomato Roots

“…Several reports have described that the secretion of extracellular proteinases plays an important role in the nematicidal activity of antagonistic strains of Bacillus , Pseudomonas , and Stenotrophomonas (9–11). We also found that the genome contains gene loci for resorcinol, terpene, arylpolyene, siderophore, and thiazole/oxazole-modified microcin (TOMM) identified by antiSMASH analysis (12); various resorcinol compounds and plantazolicin (a member of the TOMM family) have been known to be involved in nematicidal activity (13–15). Overall, the whole-genome analysis of D. acidovorans strain HK171 provides a genetic foundation for the understanding of biocontrol mechanism to root-knot nematode.…”

Secondary Metabolites with Antinematodal Activity from Higher Plants

“…Two phenolic acids, 3,5-dihydroxybenzoic acid (134) and gallic acid (141), were isolated from the aerial parts of Rubus niveus (Rosaceae) with their nematicidal activity against freshly hatched secondary stage juveniles of M. incognita [107]. Another two nematicial phenolic compounds namely vanillic acid (or called 3-methoxy-4-hydroxy benzoic acid, 140) and methyl benzoate (142) against nematode M. incognita were isolated from the aerial parts of Buddleja crispa (Loganiaceae) [108].…”